We narrowed to 11,399 results for: nar;
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Plasmid#214064PurposeFluorescent reporter system to measure EJC-enhanced NMD activity. No intron in 5'UTRDepositorInsertTurboRFP
ExpressionMammalianMutationLinker sequence present between RFP and GFPAvailable SinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYZ462
Plasmid#187970PurposeTEF1p-Cas9-CYC1t and SNR52p-Not1-SUP4tDepositorInsertCas9 (codon optimized for human)
UseSynthetic BiologyExpressionYeastPromoterTEF1Available SinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
ART
Plasmid#214063PurposeFluorescent reporter system to measure EJC-independent NMD activity. No intron in 5'UTRDepositorInsertTurboRFP
ExpressionMammalianMutationLinker sequence present between RFP and GFPAvailable SinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
ARTX_ctrl
Plasmid#214065PurposeFluorescent reporter system, NMD negative control. No intron in 5'UTRDepositorInsertTurboRFP
ExpressionMammalianMutationRemnant of linker sequence present at the end of …Available SinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
ART_RTctrl
Plasmid#214066PurposeFluorescent reporter system, readthrough control, NMD negative control. No intron in 5'UTRDepositorInsertTurboRFP
ExpressionMammalianMutationLinker sequence present between RFP and GFPAvailable SinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBWB162
Plasmid#140634PurposepBAV1k-lacI-Trc-mCherry base, lacI repression vector for ADP1DepositorInsertmCherry
ExpressionBacterialAvailable SinceSept. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBAD_Kan-LacI
Plasmid#79826PurposeArabinose induced expression of Lac repressorDepositorInsertLacI
TagsHis6ExpressionBacterialPromoteraraBpAvailable SinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBTK622
Plasmid#180592Purposetdk/kanR dual selection cassette for engineering the genome of Acinetobacter baylyi ADP1DepositorInsertstdk
kanR
UseSynthetic BiologyAvailable SinceMay 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSEVA63_Helper
Plasmid#190432PurposeM13 helper genes cloned into the pSEVA631 vector. This plasmid does not carry an M13 packaging signal. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ297.)DepositorInsertM13 genes: I-XI
UseSynthetic BiologyAvailable SinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-AncMEcoGII250
Plasmid#205025PurposeExpressing engineered M.EcoGII with higher thermostabilityDepositorInsertAncestral M.EcoGII 250
TagsHis6x tagExpressionBacterialPromoterT7Available SinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHO4d-Cas9
Plasmid#67881PurposeCas9nlsHis6 Bacterial Expression constructDepositorInsertCas9
UseCRISPRTagsHis6 and T7 epitopeExpressionBacterialPromoterT7Available SinceSept. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSB1K3_M13ps_LacZα_gIII
Plasmid#190435PurposeM13 packaging signal, LacZα gene, and M13 gIII gene (also called gp3, or g3p) cloned into the pSB1K3 vector.DepositorInsertsM13 packaging signal
M13 gIII
LacZα
UseSynthetic BiologyAvailable SinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMUH_unc84_2XGFP
Plasmid#46023DepositorInsertUNC-84 (unc-84 )
Use10xuas-phi31-expression vectorTags2XGFPMutationTwo amino acid mutations (F279L and L500P) compar…Promoter10XUASAvailable SinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDae069 (eMutaT7[PmCDA1] with optimized RBS)
Plasmid#187621PurposeExpresses eMutaT7[PmCDA1] and UGI for mutagenizing genes downstream from a T7 promoter in e.coliDepositorInsertPmCDA1-T7RNAP
ExpressionBacterialAvailable SinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIA1414
Plasmid#172402PurposeExpression of SARS-COV-2 Nsp9 in E. coliDepositorAvailable SinceAug. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pzk408-pmax-3xflag-dpspCas13b(AAAA)-NES
Plasmid#196828PurposeExpress dpdpCas13b with AAAA mutationDepositorInsertdpspCas13b(AAAA)
Tags3XFLAG and NESExpressionMammalianMutationK367A, D369A and K370AAvailable SinceMarch 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDae029 (eMutaT7[TadA-8e])
Plasmid#187620PurposeExpresses eMutaT7[TadA-8e] for mutagenizing genes downstream from a T7 promoter in e.coliDepositorInsertTadA-8e-T7RNAP
ExpressionBacterialAvailable SinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
NLS-Halo
Plasmid#200197PurposeExpression for SV40 NLSDepositorInsert3X SV40 NLS-Halo
TagsHaloExpressionMammalianMutationNonePromoterEF_1alphaAvailable SinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pB1H2w2-zif268
Plasmid#18045DepositorInsertzif268
ExpressionBacterialMutationZif268 is fused to the omega subunit of RNA polym…Available SinceJune 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
LRTi
Plasmid#214072PurposeLuminescent reporter system to measure EJC-enhanced NMD activity. No intron in 5'UTRDepositorInsertFirefly luciferase
ExpressionMammalianMutationLinker sequence present between firefly luciferas…Available SinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only