Addgene

We narrowed to 5,888 results for: pcDNA 3.1

Showing: 1321 - 1340 of 5888 results

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pcDNA3.1-FLAG-Dicer-delHelicC

Plasmid

#51366

Depositor

Narry Kim

Article

Lee et al EMBO J. 2006 Feb 8;25(3):522-32. Epub 20

Insert

Dicer (DICER1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

From N terminus to HelicC domain deletion

Promoter

CMV

Available since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+-mutTornado-split-nLuc

Plasmid

#212707

Purpose

Tornado split nLuc with a mutated 3' ribozyme so that circularization cannot occur.

Depositor

Samie Jaffrey

Article

Unti et al Cell Chem Biol. 2023 Oct 13:S2451-9456(2

Insert

mutTornado-CVB3-split-nLuc

Use

Tags

Expression

Mutation

Deleted the partial 3'ribozyme sequence in T…

Promoter

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+ eiF4A1 myc HIS

Plasmid

#71657

Purpose

mammalian expression of eiF4A1

Depositor

Gideon Dreyfuss

Insert

eiF4A1 (EIF4A1 Human)

Use

Tags

HIS and myc

Expression

Mammalian

Mutation

aa403 from Ala to Arg

Promoter

CMV

Available since

Jan. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-FLAG-Dicer-delPAZ

Plasmid

#51367

Depositor

Narry Kim

Article

Lee et al EMBO J. 2006 Feb 8;25(3):522-32. Epub 20

Insert

Dicer (DICER1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

From N terminus to PAZ domain deletion

Promoter

CMV

Available since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-FLAG-Dicer-delDEAD

Plasmid

#51364

Depositor

Narry Kim

Article

Lee et al EMBO J. 2006 Feb 8;25(3):522-32. Epub 20

Insert

Dicer (DICER1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

From N terminus to DEAD domain deletion

Promoter

CMV

Available since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-FLAG-Dicer-delDUF

Plasmid

#51365

Depositor

Narry Kim

Article

Lee et al EMBO J. 2006 Feb 8;25(3):522-32. Epub 20

Insert

Dicer (DICER1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

From N terminus to DUF domain deletion

Promoter

CMV

Available since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

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FLAG-OTUB1 C91S-pcDNA3.1

Plasmid

#118210

Purpose

Expresses FLAG-OTUB1 C91S catalytic inactive mutant in mammalian cells

Depositor

Cynthia Wolberger

Article

Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1

Insert

OTUB1 (OTUB1 Human)

Use

Tags

3x FLAG

Expression

Mammalian

Mutation

changed cysteine 91 to serine

Promoter

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/Myc-His(-)-HSulf-1

Plasmid

#13003

Depositor

Steven Rosen

Article

Morimoto-Tomita et al J Biol Chem. 2002 Dec 20. 277(51):49175-

Insert

human sulfatase 1 (SULF1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 30, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-3xFLAG-TEV-UBE2O

Plasmid

#105718

Purpose

Expresses WT full length human N- terminally tagged UBE2O

Depositor

Ramanujan Hegde

Article

Yanagitani et al Science. 2017 Aug 4;357(6350):472-475. d

Insert

UBE2O (UBE2O Human)

Use

Tags

3xFLAG-TEV

Expression

Mammalian

Mutation

Promoter

CMV (from vector)

Available since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1D V5-His-TOPO-HuR.wt

Plasmid

#110417

Purpose

Mammalian expression of HuR (wild type)

Depositor

Sandra Martha Gomes Dias

Insert

HuR (ELAVL1) (ELAVL1 Human)

Use

Tags

6xHis and V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 VDAC1-Linker-GFP

Plasmid

#211734

Purpose

Expresses GFP tagged VDAC1 in mammalian cells. GFP is attached to the C-terminus of VDAC1 with a flexible linker.

Depositor

Roland Malli

Article

Pilic et al bioRxiv 2023.09.08.556841

Insert

Voltage-dependent anion channel 1 (VDAC1 )

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-ZNF598-TEV-3xFLAG

Plasmid

#105690

Purpose

Expresses C terminally 3xFLAG tagged ZNF598 protein

Depositor

Ramanujan Hegde

Article

Juszkiewicz et al Mol Cell. 2017 Feb 16;65(4):743-750.e4.

Insert

ZNF598 (ZNF598 Human)

Use

Tags

TEV-3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV (from vector)

Available since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CMV-mCherry-hPMCA2w/b

Plasmid

#111570

Purpose

Expresses a plasma membrane calcium pump hPMCA2w/b

Depositor

Baljit Khakh

Article

Yu et al Neuron. 2018 Aug 27. pii: S0896-6273(18)

Insert

Plasma membrane calcium pump 2, variant w/b (ATP2B2 Human)

Use

Tags

mCherry

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 IGF1R-BirA(R118G)-HA

Plasmid

#109232

Purpose

This construct can be used in the BioID process to identify proteins that interact with the Insulin-like Growth Factor 1 Receptor (IGF1R).

Depositor

Victor Dzau

Article

Bareja et al J Biol Chem. 2018 Mar 12. pii: RA118.002

Insert

IGF1R (IGF1R Human)

Use

Tags

BirA(R118G) (highly promiscuous form) and HA

Expression

Mammalian

Mutation

Promoter

T7

Available since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/NDUFS7/myc-His

Plasmid

#127488

Purpose

Expresses myc-tagged NDUFS7 in mammalian cells

Depositor

Shashi Jain, Dieter Wolf

Article

Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

Insert

NDUFS7 (NDUFS7 Human)

Use

Tags

myc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 16, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/AUG-DAP5-3XFLAG

Plasmid

#127331

Purpose

Express 3xFLAG tagged DAP5 protein from AUG start codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

DAP5 (EIF4G2 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/Myc-His beta4

Plasmid

#16039

Depositor

Filippo Giancotti

Article

Dans et al J Biol Chem. 2001 Jan 12. 276(2):1494-50

Insert

beta4 (ITGB4 Human)

Use

Tags

His and Myc

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1 (-) + SLNCR1 + 12x MS2 bs

Plasmid

#86828

Purpose

Expression of SLNCR1 lncRNA tagged with 12 copies of the MS2 stem-loop sequence

Depositor

Carl Novina

Article

Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

Insert

LINC00673 (LINC00673 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 1, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/opto-a1AR-EYFP

Plasmid

#20947

Purpose

Mammalian expression of opto-a1AR fused to EYFP for optical control of GPCR signaling

Depositor

Karl Deisseroth

Article

Airan et al Nature. 2009 Mar 18. ():.

Insert

opto-a1AR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 28, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CibN-dCas9-CibN

Plasmid

#60553

Purpose

Expresses CibN-dCas9-CibN in mammalian cells

Depositor

Charles Gersbach

Article

Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

Insert

dCas9-CibN

Use

CRISPR

Tags

FLAG and SV40 NLS

Expression

Mammalian

Mutation

inactivating Cas9 mutations D10A and H840A

Promoter

CMV

Available since

Feb. 2, 2015

Availability

Academic Institutions and Nonprofits only

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