We narrowed to 54,516 results for: plasmid

1. pTre-Tight-luciferase-GFP (467)

   Plasmid

   #65491

   Purpose

   negative control for expression assays

   Depositor

   Roderic Guigo, Rory Johnson

   Insert

   EGFP
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tight TRE promoter

   Available since

   Sept. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV-syn-axon-jGCaMP8s-WPRE

   Plasmid

   #186043

   Purpose

   jGCaMP8s expression in axons under the control of the synapsin promoter

   Depositor

   Loren Looger

   Insert

   axon-jGCaMP8s
   

   Use

   AAV

   Tags

   GAP43-6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   synapsin

   Available since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV-syn-axon-jGCaMP8m-WPRE

   Plasmid

   #186042

   Purpose

   jGCaMP8m expression in axons under the control of the synapsin promoter

   Depositor

   Loren Looger

   Insert

   axon-jGCaMP8m
   

   Use

   AAV

   Tags

   GAP43-6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   synapsin

   Available since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAGGS-ROSAf

   Plasmid

   #54323

   Purpose

   TALEN expression construct for targeting the murine ROSA26 locus. It recognize the sequence: CTGCAACTCCAGTCT. Can be used with the pBS SK mCherryROSAbsr plasmid

   Depositor

   Silvestro Conticello

   Insert

   TALEN for the murine ROSA26 locus
   

   Use

   Mouse Targeting and TALEN

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAGG

   Available since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pY128 (pcDNA3.1-U6-huMb2Cpf1)

   Plasmid

   #92304

   Purpose

   Expression of humanized Mb2Cpf1 and crRNA

   Depositor

   Feng Zhang

   Insert

   huMb2Cpf1
   

   Use

   Tags

   NLS and NLS-3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAGGS-ROSAr

   Plasmid

   #54324

   Purpose

   TALEN expression construct for targeting the murine ROSA26 locus. It recognize the sequence: GGGAGTCTTCTGGGC. Can be used with the pBS SK mCherryROSAbsr plasmid

   Depositor

   Silvestro Conticello

   Insert

   TALEN for the murine ROSA26 locus
   

   Use

   Mouse Targeting and TALEN

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAGG

   Available since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pY129 (pcDNA3.1-U6-huMb3Cpf1)

   Plasmid

   #92305

   Purpose

   Expression of humanized Mb3Cpf1 and crRNA

   Depositor

   Feng Zhang

   Insert

   huMb3Cpf1
   

   Use

   Tags

   NLS and NLS-3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPD708 DsRed-Express2-ZF21

   Plasmid

   #138795

   Purpose

   Constitutive expression of DsRed-Express2-ZF21 under the CMV promoter.

   Depositor

   Joshua Leonard

   Insert

   DsRed-Express2-ZF21
   

   Use

   Synthetic Biology

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUA97

   Plasmid

   #69340

   Purpose

   It encodes a fluorescent protein that can be fused to a gene of interest using SEC CRISPR strategy

   Depositor

   Oliver Hobert

   Insert

   ceBFP SEC 3xFlag
   

   Use

   Tags

   3xFlag and ceBFP (mTagBFP)

   Expression

   Worm

   Mutation

   sqt-1(e1350)

   Promoter

   Available since

   Dec. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pY127 (pcDNA3.1-U6-huTsCpf1)

    Plasmid

    #92303

    Purpose

    Expression of humanized TsCpf1 and crRNA

    Depositor

    Feng Zhang

    Insert

    huTsCpf1
    

    Use

    Tags

    NLS and NLS-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. AAV-syn-LifeAct-jGCaMP8m-WPRE

    Plasmid

    #186039

    Purpose

    AAV-mediated expression of LifeAct-jGCaMP8m under the synapsin promoter

    Depositor

    Loren Looger

    Insert

    LifeAct-jGCaMP8m
    

    Use

    AAV

    Tags

    LifeAct-6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    synapsin

    Available since

    July 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV-syn-axon-jGCaMP8f-WPRE

    Plasmid

    #186041

    Purpose

    jGCaMP8f expression in axons under the control of the synapsin promoter

    Depositor

    Loren Looger

    Insert

    axon-jGCaMP8f
    

    Use

    AAV

    Tags

    GAP43-6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    synapsin

    Available since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pXPR_016

    Plasmid

    #107143

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and Hygro resistance from EF-1a promoter. 3rd generation lentiviral backbone.

    Depositor

    David Root

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHACK-GAL4>GAL80

    Plasmid

    #104874

    Purpose

    HACK Donor plasmids for converting GAL4 lines to GAL80 lines using HACK method.

    Depositor

    Christopher Potter

    Insert

    T2A-GAL80
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV-syn-NES-jGCaMP8m-WPRE

    Plasmid

    #186036

    Purpose

    AAV-mediated cytosolic expression of jGCaMP8m under the synapsin promoter

    Depositor

    Loren Looger

    Insert

    NES-jGCaMP8m
    

    Use

    AAV

    Tags

    NES-6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    synapsin

    Available since

    July 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. AAV-syn-NES-jGCaMP8s-WPRE

    Plasmid

    #186037

    Purpose

    AAV-mediated cytosolic expression of jGCaMP8f under the synapsin promoter

    Depositor

    Loren Looger

    Insert

    NES-jGCaMP8s
    

    Use

    AAV

    Tags

    NES-6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    synapsin

    Available since

    July 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. 4IJD

    Plasmid

    #51328

    Depositor

    Cheryl Arrowsmith

    Insert

    4IJD (PRDM9 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Truncation: See Comments

    Promoter

    T7

    Available since

    Feb. 12, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

18. pAS1-his-24::tagBFP2

    Plasmid

    #124321

    Purpose

    Expressing Nuclear Localized tagBFP2

    Depositor

    Andrew Leifer

    Insert

    tagBFP2 (his-24 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    April 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV_Ef1a_FLEX_mAPEX2_P2A_mRuby2

    Plasmid

    #171938

    Purpose

    Expresses membrane targeted (palmitoylation) APEX2 and mRuby2 as separate proteins

    Depositor

    David Berson

    Insert

    Apex2, mRuby2
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    July 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pmr101A-cPr-mCherry (ErmR)

    Plasmid

    #177206

    Purpose

    Plasmid with p15A origin for constitutive mCherry expression in E. coli

    Depositor

    Alexey Merz

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Synthetic constitutive promoter

    Available since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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