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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 2,180 results for: PAM

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Showing: 1341 - 1360 of 2180 results

  1. pCMV-T7-SpCas9-P2A-EGFP (RTW3027)

    Plasmid
    #139987
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized SpCas9 with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Promoter
    CMV and T7
    Available Since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139987-plasmid-map-sequence-id-275335

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  7. pAAV_CAG_FLEX_PKIĪ±-P2A-mRuby3_WPRE

    Plasmid
    #196677
    Purpose
    AAV plasmid for mammalian Cre-dependent expression of PKIĪ±-P2A-mRuby3 under a CAG promotor w/ WPRE enhancer. PKIĪ± (protein kinase inhibitor Ī±) inhibits the activity of protein kinase A (PKA).
    Depositor
    Haining Zhong
    Article
    Ma et al Nature. 2022 Nov;611(7937):762-768. doi:
    Insert
    PKIĪ±-P2A-mRuby3
    Use
    AAV and Cre/Lox
    Expression
    Mammalian
    Available Since
    Feb. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    196677-plasmid-map-sequence-id-385124

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Showing: 1341 - 1360 of 2180 results