Skip to main content
Addgene
Showing: 1341 - 1360 of 1475 results

  6. pMB1_1R26PylRS(CbzK)_AfTyrRS(p-I-Phe)_AlvtRNA-ΔNPyl(8)(CGA)_AftRNA-Tyr(A01)(CUA)

    Plasmid
    #174516
    Purpose
    Recoded double aaRS/tRNA plasmid with 1R26PylRS/AlvtRNA-ΔNPyl(8)(CGA) & AfTyrRS/AftRNA-Tyr(A01)(CUA) to incorporate CbzK into the TCG codon & p-I-Phe into the TAG codon.
    Depositor
    Jason W Chin
    Article
    Robertson et al Science. 2021 Jun 4;372(6546):1057-1062.
    Inserts
    PylS_1R26 - CBZK
    Af_TyrRS - p-I-Phe
    Ma tRNA pylT(8) with CGA anticodon
    A.fulgidus tRNA with CUA anticodon
    Use
    Tags
    Expression
    Bacterial
    Mutation
    CUA anticodon, M.alvus Pyl tRNA with CGA anticodo…
    Promoter
    GlnS and lpp
    Available since
    Oct. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    174516-plasmid-map-sequence-id-343328

    Enlarge

  9. mTLR4

    Plasmid
    #13085
    Depositor
    Ruslan Medzhitov
    Insert
    TLR4 (Tlr4 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    13085-plasmid-map-sequence-id-142564

    Enlarge

  10. pAttB-Tub

    Plasmid
    #203966
    Purpose
    Fly transformation vector with Tubulin promoter and SV40 PA site and a UTR for ubiquitous expression
    Depositor
    Andreas Jenny
    Type
    Empty backbone
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Oct. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    203966-plasmid-map-sequence-id-403023

    Enlarge

  11. mTLR4 flag

    Plasmid
    #13087
    Depositor
    Ruslan Medzhitov
    Insert
    TLR4 (Tlr4 Mouse)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Amino acids 1-19 have been replaced with mammalia…
    Promoter
    Available since
    Oct. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    13087-plasmid-map-sequence-id-147760

    Enlarge

  12. pnos-Fok1:dCas9-nos

    Plasmid
    #62210
    Purpose
    Expresses Fok1:dCas9 under control of nanos promoter and 3'UTR. For germ line restricted high specificity genome engineering in Drosophila melanogaster.
    Depositor
    Simon Bullock
    Insert
    Fok1-dCas9
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    nanos
    Available since
    March 12, 2015
    Availability
    Academic Institutions and Nonprofits only
    62210-plasmid-map-sequence-id-101869

    Enlarge

  13. 4MX2

    Plasmid
    #60102
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    LdBPK_040440.1:MAC04A-B07:C226244
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  14. pCAG-EnvARGCD

    Plasmid
    #100800
    Purpose
    express fusion protein of EnvA (ASLV-A envelope protein) extracellular and transmembrane domains with rabies virus SAD B19 strain glycoprotein intracellular domain
    Depositor
    Ian Wickersham
    Insert
    fusion protein of EnvA extracellular and transmembrane domain with rabies virus glycoprotein intracellular domain
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Dec. 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    100800-plasmid-map-sequence-id-189516

    Enlarge

  15. pLV-Blast_hCOL4A5

    Plasmid
    #198100
    Purpose
    lentiviral expression of hCOL4A5 in mammalian cells
    Depositor
    Jeffrey Miner
    Insert
    COL4A5 (COL4A5 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    EF1a
    Available since
    March 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    198100-plasmid-map-sequence-id-389263

    Enlarge

  16. pCMV5 HA-TGFBRII

    Plasmid
    #19158
    Depositor
    Joan Massague
    Insert
    Transforming growth factor, beta receptor II (TGFBR2 Human)
    Use
    Tags
    HA (YDVPDYASL)
    Expression
    Mammalian
    Mutation
    PCR based mutagenesis
    Promoter
    Available since
    Oct. 1, 2008
    Availability
    Academic Institutions and Nonprofits only
    19158-plasmid-map-sequence-id-364716

    Enlarge

  17. pLV-Blast_hCOL4A5-HA

    Plasmid
    #198103
    Purpose
    lentiviral expression of hCOL4A5 in mammalian cells
    Depositor
    Jeffrey Miner
    Insert
    COL4A5 (COL4A5 Human)
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mutation
    Promoter
    EF1a
    Available since
    March 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    198103-plasmid-map-sequence-id-389259

    Enlarge

  18. pLV-Blast_hCOL4A5-HA_C1567R

    Plasmid
    #198105
    Purpose
    lentiviral expression of hCOL4A5_C1567R in mammalian cells
    Depositor
    Jeffrey Miner
    Insert
    COL4A5 (COL4A5 Human)
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mutation
    C1567R
    Promoter
    EF1a
    Available since
    March 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    198105-plasmid-map-sequence-id-389261

    Enlarge

  19. pLV-Blast_hCOL4A5-HA_G869R

    Plasmid
    #198104
    Purpose
    lentiviral expression of hCOL4A5_G869R in mammalian cells
    Depositor
    Jeffrey Miner
    Insert
    COL4A5 (COL4A5 Human)
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mutation
    G869R
    Promoter
    EF1a
    Available since
    March 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    198104-plasmid-map-sequence-id-389260

    Enlarge

  20. TRUPATH

    Plasmid Kit
    #1000000163
    Purpose
    A collection of 20 plasmids encoding alpha, beta, or gamma subunits of the heterotrimeric G protein complex for detection of heterotrimer dissociation. GPCR
    Depositor
    Bryan Roth
    Available since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
Showing: 1341 - 1360 of 1475 results