We narrowed to 10,582 results for: plasmids 101

1. pVH237-2-Tier1-PmPGK1-TtgA

   Plasmid

   #169584

   Purpose

   Tier-1 vector encoding PmPGK1-driven TtgA expression (PmPGK1-TtgA-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   Regulatory protein TtgR fused to a VP16 domain
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PPGK

   Available Since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTS2367-Tier1-PhCMV_VP16

   Plasmid

   #169568

   Purpose

   Tier-1 vector encoding PhCMV-driven VP16 transactivator domain (PhCMV-VP16-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven VP16
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV

   Available Since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFOX8-Tier1-PhCMV-YPet

   Plasmid

   #169506

   Purpose

   Tier-1 vector encoding PhCMV-driven YPet expression (PhCMV-YPet-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven yellow fluorescent protein Ypet
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV

   Available Since

   June 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTS2401-Tier1-PhCMV-HygroR

   Plasmid

   #169493

   Purpose

   Tier-1 vector encoding PhCMV-driven hygromycin resistance gene HygroR (PhCMV-HygroR-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven hph
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV

   Available Since

   June 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTS2415-Tier1-PPGK-1xEAAAK

   Plasmid

   #169482

   Purpose

   Tier-1 vector encoding PPGK-controlled 1x (AEAAAK)-linker (PPGK-AEAAAK-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PPGK-driven EAAAK-linker
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PPGK

   Available Since

   June 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTS2412-Tier1-PPGK-2xGS

   Plasmid

   #169481

   Purpose

   Tier-1 vector encoding PPGK-controlled 2x (GGGGS)-linker (PPGK-2xGS-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PPGK-driven GS-linker
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PPGK

   Available Since

   June 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJL50

   Plasmid

   #174068

   Purpose

   Expresses 97-3 zinc finger array fused to VP16 in S. cerevisiae. Parent plasmid for chromatin regulator library.

   Depositor

   Albert Keung

   Article

   Lee et al Cell Syst. 2021 Aug 24. pii: S2405-4712(

   Insert

   pTEF1-3NLS-97-3ZF-VP16
   

   Use

   Tags

   FLAG

   Expression

   Yeast

   Mutation

   Promoter

   pTEF1

   Available Since

   Dec. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. Rab22a cds in pGem-T Easy

   Plasmid

   #86223

   Purpose

   Can be used for in situ hybridisation or other purposes

   Depositor

   Rob Parton

   Article

   Hall et al Cell Logist. 2017 Mar 1;7(1):e1301151. d

   Insert

   Rab22a (rab22a Zebrafish)
   

   Use

   In situ probe/other

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV-CMV-scFVtet1_bGHpA

   Plasmid

   #177351

   Purpose

   AAV expression of scFV-fused catalytic domain of TET1 for targeted DNA methylation editing

   Depositor

   Naoki Yamamoto

   Article

   Yamamoto et al Research Square [Preprint] 2021. doi: 10

   Insert

   Tet Methylcytosine Dioxygenase 1 (TET1 Human)
   

   Use

   AAV

   Tags

   myc and scFV

   Expression

   Mammalian

   Mutation

   N terminal domain of human Tet1

   Promoter

   CMV promoter

   Available Since

   Sept. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAV-TRE-scFVtet1_bGHpA

    Plasmid

    #177357

    Purpose

    AAV expression of scFV-fused catalytic domain of TET1 from doxycycine-inducible promoter for targeted DNA methylation editing

    Depositor

    Naoki Yamamoto

    Article

    Yamamoto et al Research Square [Preprint] 2021. doi: 10

    Insert

    Tet Methylcytosine Dioxygenase 1 (TET1 Human)
    

    Use

    AAV

    Tags

    myc and scFV

    Expression

    Mammalian

    Mutation

    N terminal domain of human Tet1

    Promoter

    Tetracycline-dependent promoter

    Available Since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS

    Plasmid

    #60904

    Purpose

    The plasmid encodes a antibody that binds to the GCN4 peptide from the SunTag system, and is fused to a transcriptional activation domain VP64

    Depositor

    Ron Vale

    Article

    Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

    Insert

    scFv-GCN4
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFastBac-Dual-M2

    Plasmid

    #135584

    Purpose

    High eukaryotic dual protein expression shuttle vector

    Depositor

    Xiao-Wen Cheng

    Article

    Shang et al J Biotechnol. 2017 Aug 10;255:37-46. doi

    Type

    Empty backbone

    Use

    Transfer vector

    Tags

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin of AcMNPV

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. CAG-Blimp1

    Plasmid

    #73995

    Purpose

    Plasmid expressing mouse Blimp1

    Depositor

    Connie Cepko

    Article

    Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

    Insert

    Blimp1 gene (Prdm1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGDP1 uvrA

    Plasmid

    #112892

    Purpose

    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

    Depositor

    Gerard Wright

    Article

    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

    Insert

    uvrA-like protein
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Pbla

    Available Since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLVU/RED

    Plasmid

    #24178

    Depositor

    Lars Ittner

    Article

    Krupka et al Plasmid. 2010 May;63(3):155-60. doi: 10.

    Insert

    Chloramphenicol resistance gene (CmR) - ccdB gene
    

    Use

    Lentiviral; Gateway

    Tags

    DsRED

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 23, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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16. p1D4-hrGFP II

    Plasmid

    #71317

    Purpose

    expression vector with 1D4 epitope added as a C-terminal tag, allowing for in vitro expression of any visual pigment or GPCR of interest.

    Depositor

    Belinda Chang

    Article

    Morrow et al Plasmid. 2010 Nov;64(3):162-9. doi: 10.1

    Type

    Empty backbone

    Use

    Tags

    1D4 epitope (TETSQVAPA) and IRES-hrGFPII

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pHBJT001

    Plasmid

    #225152

    Purpose

    High copy cloning vector for integration of C-terminal FLAG epitope tag. SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in same orientation as lac promoter.

    Depositor

    Jenny-Lee Thomassin

    Article

    Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    FLAG epitope tag (35 bp)

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-EF1a-fDIO-stGtACR2-FusionRed

    Plasmid

    #217975

    Purpose

    AAV transfer plasmid to express stGtACR2-FusionRed in a frt-dependent manner

    Depositor

    Edward Callaway

    Article

    Kirchgessner et al Curr Biol. 2021 Dec 6;31(23):5121-5137.e

    Insert

    stGtACR2-FusionRed
    

    Use

    AAV

    Tags

    FusionRed

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTREX GPR68_NB2_2

    Plasmid

    #127691

    Purpose

    Plasmid contains a nanobody that directly interacts with the cytoplasmic surface of GPR68.

    Depositor

    Justin English, Bryan Roth

    Article

    English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306

    Insert

    GPR68_NB2_2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pVH231-Tier1-PmPGK1-mTagBFP2

    Plasmid

    #169520

    Purpose

    Tier-1 vector encoding PmPGK1-driven mTagBFP2 expression (PmPGK1-mTagBFP2-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PPGK-driven monomeric Tag blue fluorescent protein 2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PPGK

    Available Since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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