We narrowed to 2,957 results for: BFP

1. iE65 PB-Zim3-XTEN-dCas9-mApple-puro-BFP

   Plasmid

   #204724

   Purpose

   Constitutive CRISPRi by Zim3-dCas9

   Depositor

   Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

   Insert

   Zim3-dCas9
   

   Expression

   Mammalian

   Available Since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCA20-HSPB1 5'HA-mTagBFP-3'-UTR 12XMS2V5-3'HA (HDR donor)

   Plasmid

   #199451

   Purpose

   CRISPR donor plasmid to insert [mTagBFP-stop-12XMS2] into the C-terminus of human HSPB1 gene; 12XMS2 locates in the 3' UTR region

   Depositor

   Baohui Chen

   Article

   Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

   Insert

   HDR donor template
   

   Use

   CRISPR

   Available Since

   May 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. EK0425 CMV-TO-BFPmut-stop-Bdnf.t+54@202 (FLP-IN)

   Plasmid

   #191147

   Purpose

   Expression of the sequence matching the 54 extra bases in EK0362 in mammalian cells

   Depositor

   Xiaojing Gao

   Article

   Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

   Insert

   Bdfn.t+54
   

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CMV-TO

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-TagBFP-P2A-Pre-SPHERE-Rncp-iGluSnFR1

   Plasmid

   #187093

   Purpose

   Glutamate sensor for localization to membrane contatct

   Depositor

   Kotaro Oka

   Article

   Shindo et al bioRxiv 2022

   Insert

   Pre-SPHERE-Rncp-iGluSnFR1
   

   Tags

   TagBFP-P2A

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHIE061 ZF43x6-C mKate2 EF1a EBFP2-P2A-BlastR

   Plasmid

   #138742

   Purpose

   Integration Vector, assembled with pHIE045 (TUPV1), pJM451 (TUPV2), pPD612

   Depositor

   Joshua Leonard

   Article

   Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

   Insert

   ZF43x6-Compact
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Available Since

   March 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHIE065 ZF43x12-S mKate2 EF1a EBFP2-P2A-BlastR

   Plasmid

   #138746

   Purpose

   Integration Vector, assembled with pHIE049 (TUPV1), pJM451 (TUPV2), pPD612

   Depositor

   Joshua Leonard

   Article

   Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

   Insert

   ZF43x12-Spaced
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Available Since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCFJ1972 - [1-2] ENTR - Flour - TagBFP2(NLS(2), no_atg, no_stop)

   Plasmid

   #159844

   Purpose

   Three-fragment Gateway compatible flourophore for expression in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   [1-2] ENTR - Flour - TagBFP2(NLS(2), no_atg, no_stop)
   

   Expression

   Worm

   Mutation

   Not applicable

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHIE059 ZF43x1 mKate2 EF1a EBFP2-P2A-BlastR

   Plasmid

   #138740

   Purpose

   Integration Vector, assembled with pHIE043 (TUPV1), pJM451 (TUPV2), pPD612

   Depositor

   Joshua Leonard

   Article

   Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

   Insert

   ZF43x1
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Available Since

   Dec. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHIE060 ZF43x3-C mKate2 EF1a EBFP2-P2A-BlastR

   Plasmid

   #138741

   Purpose

   Integration Vector, assembled with pHIE044 (TUPV1), pJM451 (TUPV2), pPD612

   Depositor

   Joshua Leonard

   Article

   Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

   Insert

   ZF43x3-Compact
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Available Since

   Dec. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHIE063 ZF43x3-S mKate2 EF1a EBFP2-P2A-BlastR

    Plasmid

    #138744

    Purpose

    Integration Vector, assembled with pHIE047 (TUPV1), pJM451 (TUPV2), pPD612

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    ZF43x3-Spaced
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    Dec. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHIE064 ZF43x6-S mKate2 EF1a EBFP2-P2A-BlastR

    Plasmid

    #138745

    Purpose

    Integration Vector, assembled with pHIE048 (TUPV1), pJM451 (TUPV2), pPD612

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    ZF43x6-Spaced
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    Dec. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHIE062 ZF43x12-C mKate2 EF1a EBFP2-P2A-BlastR

    Plasmid

    #138743

    Purpose

    Integration Vector, assembled with pHIE046 (TUPV1), pJM451 (TUPV2), pPD612

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    ZF43x12-Compact
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHBS1506 [IBB-GFP-mCherry3E]-[BFP-TDP43 FYW-L]

    Plasmid

    #133330

    Purpose

    To test the effect of sequence on TDP43 splicing activity

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    TARDBP mutant (TARDBP Human)
    

    Expression

    Mammalian

    Mutation

    All FYW to L in CTD of full-length TDP43

    Available Since

    Nov. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHBS1507 [IBB-GFP-mCherry 3E]-[BFP-TDP43 VLIM-S]

    Plasmid

    #133331

    Purpose

    To test the effect of sequence on TDP43 splicing activity

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    TARDBP mutant (TARDBP Human)
    

    Expression

    Mammalian

    Mutation

    All VLIM to S in CTD of full-length TDP43

    Available Since

    Nov. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHBS1508 [IBB-GFP-mCherry 3E]-[BFP-TDP43 VLIM-F]

    Plasmid

    #133332

    Purpose

    To test the effect of sequence on TDP43 splicing activity

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    TARDBP mutant (TARDBP Human)
    

    Expression

    Mammalian

    Mutation

    All VLIM to F in CTD of full-length TDP43

    Available Since

    Nov. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHBS1401 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 F-S]

    Plasmid

    #118808

    Purpose

    To test the effect of sequence on TDP43 splicing activity

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    TARDBP mutant (TARDBP Human)
    

    Expression

    Mammalian

    Mutation

    All F to S in CTD of full-length TDP43

    Available Since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHBS1187 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 N-Q]

    Plasmid

    #107847

    Purpose

    To test the effect of sequence on TDP43 splicing activity

    Depositor

    Rajat Rohatgi

    Insert

    TARDBP mutant (TARDBP Human)
    

    Expression

    Mammalian

    Mutation

    All asparagine to glutamine in splicing reporter

    Available Since

    April 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHBS1185 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 F-S]

    Plasmid

    #107843

    Purpose

    To test the effect of sequence on TDP43 splicing activity

    Depositor

    Rajat Rohatgi

    Insert

    TARDBP mutant (TARDBP Human)
    

    Expression

    Mammalian

    Mutation

    All phenylalanine to serine in splicing reporter

    Available Since

    April 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE

    Plasmid

    #60910

    Purpose

    dCas9 fused to 24 copies of the GCN4 peptide v4, which is part of the SunTag system

    Depositor

    Ron Vale

    Article

    Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    2x NLS, HA, and NLS

    Expression

    Mammalian

    Promoter

    dSV40 Promoter

    Available Since

    Nov. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pXJ219-pAAV-PB3'IR-U6-gRNA-EF1a-mtagBFP2-KASH-PB5'IR

    Plasmid

    #210525

    Purpose

    AAV piggybac transposon vector, with mtagBFP-KASH reporter and gRNA cloning site. Compatible with 10X 5' gRNA capture technology.

    Depositor

    Xin Jin

    Article

    Zheng et al Cell. 2024 Jun 20;187(13):3236-3248.e21.

    Inserts

    mtagBFP-KASH
    U6-gRNA
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Feb. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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