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MTK8a_005

Plasmid

#123889

Purpose

Encodes the F1 Kanamycin resistance cassette as a type 8a part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

F1-KanR-ColE1

Expression

Mammalian

Available Since

Feb. 17, 2020

Availability

Academic Institutions and Nonprofits only

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MTK234_030

Plasmid

#123910

Purpose

Encodes the spCas9 sgRNA1 hCLYBL (site 1) gRNA expression cassette as a type 234 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

sgRNA1 -hCLYBL

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK6_003

Plasmid

#123857

Purpose

Encodes pCMV-Blasticidin resistance cassette as a type 6 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

CMV-Blast-bgPA

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub-BsmBI

Plasmid

#162801

Purpose

STELLA destination plasmid containing restriction sites for golden gate cloning. Suitable for amber suppression (4xMmaPylT cassette); for transient expression

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-BsmBI

Use

Piggybac

Expression

Mammalian

Promoter

EF1alpha

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pTEI128

Plasmid

#126209

Purpose

Module 1 vector of PlaMiNGo toolkit for cloning of candidate gene at N-terminus of GFP11x3 and expression in plant cell

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Inserts

ccdB Cassette (BsaI)
GFP11x3 (5 aa linker)

Use

Binary, module 1

Expression

Plant

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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MTK234_037

Plasmid

#123912

Purpose

Encodes the spCas9 UAS (miniCMV core) gRNA expression cassette as a type 234 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

sgRNA-pUAS

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK234_041

Plasmid

#123916

Purpose

Encodes the spCas9 mScarlet gRNA expression cassette as a type 234 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

mScarlet sgRNA

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK7_004

Plasmid

#123872

Purpose

Encodes the 3rd gen. 3' HIV LTR cassette as a type 7 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

3' HIV LTR (3rd gen.)

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pEPOR2KN0039

Plasmid

#117606

Purpose

Level2 MoClo construct, containing level1 plant expression cassettes for AsCas12a(pEPOR1CB0014) and sgRNA_AtADH1 {AT1G77120}(pEPOR1CB0028)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

[35S:AsCas12a(pEPOR1CB0014) ] +[AtU6-26:sgRNA]

Expression

Plant

Available Since

Nov. 16, 2018

Availability

Academic Institutions and Nonprofits only

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MTK8a_004

Plasmid

#123888

Purpose

Encodes the F1 Ampicillin resistance cassette as a type 8a part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

F1-AmpR-ColE1

Expression

Mammalian

Available Since

April 10, 2020

Availability

Academic Institutions and Nonprofits only

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