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Showing: 61 - 70 of 293 results

  1. pSLPB2B-ecDHFR-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

    Plasmid
    #187950
    Purpose
    ecDHFR degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.
    Depositor
    Edda Schulz
    Article
    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:
    Insert
    ecDHFR-SpdCas9-tagRFPt-P2A-tagBFP
    Use
    CRISPR and Synthetic Biology
    Tags
    GGS linker
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    187950-plasmid-map-sequence-id-373501

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  2. XZ375 EF1a-IL2Rbsp-3xFLAG-IL2Rb(ECD+TM)-TS-ADAR2dd-export

    Plasmid
    #233386
    Purpose
    Optimized IL-2-sensing eLIDAR with IL-2 beta chain ecto+TM domains fused with ADAR2dd, with Golgi traffic sequence and ER export sequence
    Depositor
    Xiaojing Gao
    Article
    Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038
    Insert
    IL2Rb(ecto+TM)-ADAR2dd
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    E488Q, T501A on ADAR2dd
    Promoter
    EF1a
    Available since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    233386-plasmid-map-sequence-id-466377

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  5. pPB-Ins-TRE3Gp-KRAB-dCas9-ecDHFR-IRES-GFP-EF1Ap-Puro

    Plasmid
    #183410
    Purpose
    piggyBAC-based doxycycline- and trimethoprim-inducible KRAB-dCas9 CRISPR interference plasmid for enhancer and promoter repression in mammalian cells
    Depositor
    Azim Surani
    Article
    Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d
    Insert
    KRAB-dCas9-ecDHFR
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G
    Available since
    May 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    183410-plasmid-map-sequence-id-361256

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  8. pHR_PGK_SNIPR_CD8alpha SNIPR

    Plasmid
    #188357
    Purpose
    Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor
    Depositor
    Kole Roybal
    Article
    Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.
    Insert
    PGK_antiCD19_CD8alpha-ECD_Notch1-TMD_Notch1-JMD_Gal4VP64
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    188357-plasmid-map-sequence-id-371155

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  9. pHR_PGK_SNIPR_hsNotch1 LNR

    Plasmid
    #188336
    Purpose
    Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a Notch1-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor
    Depositor
    Kole Roybal
    Article
    Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.
    Insert
    PGK_antiCD19_Notch1-ECD_Notch1-TMD_Notch1-JMD_Gal4VP64
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    188336-plasmid-map-sequence-id-370775

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  10. pHR_PGK_SNIPR_CD28 SNIPR

    Plasmid
    #188358
    Purpose
    Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD28-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor
    Depositor
    Kole Roybal
    Article
    Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.
    Insert
    PGK_antiCD19_CD28-ECD_Notch1-TMD_Notch1-JMD_Gal4VP64
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    188358-plasmid-map-sequence-id-370848

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Showing: 61 - 70 of 293 results