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We narrowed to 236 results for: AsCas12a

Showing: 121 - 140 of 236 results

  1. pEPOR1CB0014

    Plasmid
    #117550
    Purpose
    Level 1 Golden Gate Cassette: AsCas12a expression cassette for dicotyledonous plants
    Depositor
    Nicola Patron
    Article
    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do
    Insert
    2x35S+5'UTR OMEGA (pICH51288) + AsCas12a (with stop codon)+ Nos (pICH41421)
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    2x35S+5'UTR OMEGA (pICH51288)
    Available since
    Nov. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    117550-plasmid-map-sequence-id-228088

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  7. pEPOR1CB0014

    Plasmid
    #117550
    Purpose
    Level 1 Golden Gate Cassette: AsCas12a expression cassette for dicotyledonous plants
    Depositor
    Nicola Patron
    Article
    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do
    Insert
    2x35S+5'UTR OMEGA (pICH51288) + AsCas12a (with stop codon)+ Nos (pICH41421)
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    2x35S+5'UTR OMEGA (pICH51288)
    Available since
    Nov. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    117550-plasmid-map-sequence-id-228088

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  8. pAsCpf1GG

    Plasmid
    #131011
    Purpose
    Backbone plasmid for generating CRISPR arrays for AsCas12a. Contains a GFP-dropout cassette and a direct repeat.
    Depositor
    Chase Beisel
    Article
    Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:
    Inserts
    direct repeat of AsCas12a
    GFP expression cassette
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    131011-plasmid-map-sequence-id-258106

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  9. pAsCpf1GG

    Plasmid
    #131011
    Purpose
    Backbone plasmid for generating CRISPR arrays for AsCas12a. Contains a GFP-dropout cassette and a direct repeat.
    Depositor
    Chase Beisel
    Article
    Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:
    Inserts
    direct repeat of AsCas12a
    GFP expression cassette
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    131011-plasmid-map-sequence-id-258106

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  12. pYPQ220-Ultra

    Plasmid
    #176889
    Purpose
    Gateway entry plasmid (attL1 & attR5) expressing AsCas12a with M537R and F870L mutations, without promoter
    Depositor
    Yiping Qi
    Article
    Zhang et al Front Genome Ed. 2022 Jan 31;4:780238. d
    Insert
    AsCas12a
    Use
    CRISPR; Gateway compatible cas12a entry clone
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Expression
    Plant
    Mutation
    Promoter
    Available since
    April 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    176889-plasmid-map-sequence-id-350058

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  14. pRDA52_Lenti.puro.U6_modified DR

    Plasmid
    #196723
    Purpose
    For cloning pairs of AsCas12a guides with two DRs that are as different as possible.
    Depositor
    Bernhard Schmierer
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196723-plasmid-map-sequence-id-389696

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  15. pYPQ220-Ultra

    Plasmid
    #176889
    Purpose
    Gateway entry plasmid (attL1 & attR5) expressing AsCas12a with M537R and F870L mutations, without promoter
    Depositor
    Yiping Qi
    Article
    Zhang et al Front Genome Ed. 2022 Jan 31;4:780238. d
    Insert
    AsCas12a
    Use
    CRISPR; Gateway compatible cas12a entry clone
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Expression
    Plant
    Mutation
    Promoter
    Available since
    April 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    176889-plasmid-map-sequence-id-350058

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  18. pMB22.SV40.Zeo

    Plasmid
    #135692
    Purpose
    Lentivirus delivery for stable expression of AsCas12a-VPR, Zeocin selection
    Depositor
    Florian Karreth
    Insert
    mammalian codon-optimized Acidaminococcus sp. Cas12a fused to VPR activator
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    135692-plasmid-map-sequence-id-322223

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  19. pMB22.SV40.Zeo

    Plasmid
    #135692
    Purpose
    Lentivirus delivery for stable expression of AsCas12a-VPR, Zeocin selection
    Depositor
    Florian Karreth
    Insert
    mammalian codon-optimized Acidaminococcus sp. Cas12a fused to VPR activator
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    135692-plasmid-map-sequence-id-322223

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  20. pLCHKOv3-intergenic-As

    Plasmid
    #209026
    Purpose
    Lentiviral vector expressing U6 driven hybrid guide (hgRNA) targeting two intergenic sites in the human genome using SpCas9 and AsCas12a nucleases (CHyMErA system), respectively
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    intergenic hgRNA with SpCas9 tracrRNAv1 & AsCas12a DR
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    209026-plasmid-map-sequence-id-413692

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Showing: 121 - 140 of 236 results