Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 23,893 results for: CRISPR

Showing: 121 - 140 of 23893 results

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pCRISPR3_BC2

Plasmid

#191857

Purpose

Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: ATCATG) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

Depositor

Kimberly Reynolds

Article

Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

Type

Empty backbone

Use

CRISPR

Available Since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pCRISPR3_BC6

Plasmid

#191861

Purpose

Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: CCTAGT) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

Depositor

Kimberly Reynolds

Article

Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

Type

Empty backbone

Use

CRISPR

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCRISPR3_BC5

Plasmid

#191860

Purpose

Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: AGTCTA) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

Depositor

Kimberly Reynolds

Article

Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

Type

Empty backbone

Use

CRISPR

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCRISPR3_BC4

Plasmid

#191859

Purpose

Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: GTATGA) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

Depositor

Kimberly Reynolds

Article

Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

Type

Empty backbone

Use

CRISPR

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCRISPR3_BC3

Plasmid

#191858

Purpose

Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: GCATGG) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

Depositor

Kimberly Reynolds

Article

Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

Type

Empty backbone

Use

CRISPR

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCRISPR3_BC1

Plasmid

#191856

Purpose

Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: CTTTCA) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

Depositor

Kimberly Reynolds

Article

Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

Type

Empty backbone

Use

CRISPR

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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CRISPR_HLA_DPB

Plasmid

#164989

Purpose

Expression of gRNA targeting HLA-DPB locus, including DPB1*01:01:01

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

gRNA against HLA-DPB1*01:01:01

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 29, 2022

Availability

Academic Institutions and Nonprofits only

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CRISPR_HLA_DQB

Plasmid

#164991

Purpose

Expression of gRNA targeting HLA-DQB locus, including DQB1*05:01:01

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

gRNA against HLA-DQB1*05:01:01

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 29, 2022

Availability

Academic Institutions and Nonprofits only

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CRISPR_HLA_DRB

Plasmid

#164992

Purpose

Expression of gRNA targeting HLA-DRB locus, including DRB1*01:02:01

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

gRNA against HLA-DRB1*01:02:01

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 29, 2022

Availability

Academic Institutions and Nonprofits only

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CRISPR_HLA_DQA

Plasmid

#164990

Purpose

Expression of gRNA targeting HLA-DQA locus, including DQA1*01:01:01

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

gRNA against HLA-DQA1*01:01:01

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 29, 2022

Availability

Academic Institutions and Nonprofits only

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CRISPR_HLA_DPA

Plasmid

#164988

Purpose

Expression of gRNA targeting HLA-DPA locus, including DPA1*02:01:08

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

gRNA against HLA-DPA1*02:01:08

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCRISPR2_wt

Plasmid

#149386

Purpose

P. aeruginosa PA14 CRISPR2 locus

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

Insert

Pseudomonas aeruginosa PA14 CRISPR2 locus

Expression

Bacterial

Promoter

pBAD

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pCRISPR2_-6D

Plasmid

#149387

Purpose

P. aeruginosa PA14 CRISPR2 locus, with 6bp deletion downstream of IHFprox site

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

Insert

Pseudomonas aeruginosa PA14 CRISPR2 locus

Expression

Bacterial

Mutation

6bp deleted downstream of the IHFprox site

Promoter

pBAD

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pCRISPR2_-5D

Plasmid

#149388

Purpose

P. aeruginosa PA14 CRISPR2 locus, with 5bp deletion downstream of IHFprox site

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

Insert

Pseudomonas aeruginosa PA14 CRISPR2 locus

Expression

Bacterial

Mutation

5bp deleted downstream of the IHFprox site

Promoter

pBAD

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pCRISPR2_+5D

Plasmid

#149390

Purpose

P. aeruginosa PA14 CRISPR2 locus, with 5bp addition downstream of IHFprox site

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

Insert

Pseudomonas aeruginosa PA14 CRISPR2 locus

Expression

Bacterial

Mutation

5bp inserted downstream of the IHFprox site

Promoter

pBAD

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pCRISPR2_IHF_Opt

Plasmid

#149395

Purpose

P. aeruginosa PA14 CRISPR2 locus, with "optimized" IHFprox site

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

Insert

Pseudomonas aeruginosa PA14 CRISPR2 locus

Expression

Bacterial

Mutation

IHFprox site is optimized at key positions needed…

Promoter

pBAD

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pCRISPR2_IHF_Mut

Plasmid

#149396

Purpose

P. aeruginosa PA14 CRISPR2 locus, with a mutated IHFprox site, that should not be recognized by IHF

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

Insert

Pseudomonas aeruginosa PA14 CRISPR2 locus

Expression

Bacterial

Mutation

IHFprox site is mutated at key positions needed f…

Promoter

pBAD

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pCRISPR2_motif_scram

Plasmid

#149397

Purpose

P. aeruginosa PA14 CRISPR2 locus, with a scrambled Upstream recognition motif

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

Insert

Pseudomonas aeruginosa PA14 CRISPR2 locus

Expression

Bacterial

Mutation

Upstream motif recognized by Cas1-2/3 during spac…

Promoter

pBAD

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pCRISPR

Plasmid

#186276

Purpose

Plasmid containing shortened version of upstream Thiomicrospira CRISPR array for integration assays

Depositor

Jennifer Doudna

Article

Wang et al Nat Commun. 2021 May 6;12(1):2571. doi:

Insert

Thiomicrospira shortened CRISPR array

Expression

Bacterial

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv1_COX4

Plasmid

#177983

Purpose

lentiviral vector expressing Cas9 and a sgRNA targeting COX4

Depositor

David Sabatini, Jessica Spinelli

Article

Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

Insert

sgRNA targeting COX4

Use

Lentiviral

Expression

Mammalian

Mutation

N/A

Promoter

U6

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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Showing: 121 - 140 of 23893 results

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