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Plasmid#215927PurposeFragmid fragment: (guide cassette) guide expressionDepositorHas ServiceCloning Grade DNAInsertU6_v1; DR_v0 [EnAs]; BsmBI_v2; BsmBI_v3
UseCRISPR; FragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA102
Plasmid#215935PurposeFragmid fragment: (guide cassette) guide expression; reverse orientation; enables iBar UMIsDepositorHas ServiceCloning Grade DNAInsertrev{U6_v1; DR_v0 [EnAs]; BsmBI_v2; BsmBI_v5}
UseCRISPR; FragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA107
Plasmid#215936PurposeFragmid fragment: (guide cassette) guide expression; enables iBar UMIsDepositorHas ServiceCloning Grade DNAInsertU6_v1; DR_v0 [EnAs]; BsmBI_v2; BsmBI_v5
UseCRISPR; FragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA048
Plasmid#216016PurposeFragmid fragment: (Cas protein) deactivated CasDepositorHas ServiceCloning Grade DNAInsertdCas12a (D908A)_v1.1 [EnAs]
UseCRISPR; FragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRDB_131
Plasmid#216095PurposeCas12a [EnAs] CRISPRa targeting CD97, CD4, CD26, CD274, positive controlDepositorInsertCD97, CD4, CD26, CD274 guides
UseCRISPR and Lentiviral; Positive controlsTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRDA_816
Plasmid#216098PurposeCRISPRa, EF1a-driven ALFA-dCas12a-VP64 (Cas only)DepositorInsertCas12a [EnAs]
UseCRISPR and Lentiviral; Assembled vectorTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET30Xa-SBL1(160S)
Plasmid#66872PurposeExpresses WTsoybean lipoxygenase-1 160S with N-term HistagDepositorInsertsoybean lipoxygenase-1 wild type (NEWENTRY Glycine max)
UseTagsHistag from pET30Xa-LICExpressionBacterialMutationnonePromoterT7lacAvailable sinceAug. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET30Xa-SBL1(160E)
Plasmid#66846PurposeExpresses soybean lipoxygenase-1 S160E mutant with N-term HistagDepositorUseTagsHis-tagExpressionBacterialMutationS160EPromoterT7lacAvailable sinceSept. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET30Xa-SBL1(160S,NoCys)
Plasmid#66877PurposeExpresses a cysteine-free version of WTsoybean lipoxygenase-1 160S (4Cys to Ser) with N-term HistagDepositorInsertsoybean lipoxygenase-1
UseTagsHistagExpressionBacterialMutationfour (all) cysteines mutated to serinePromoterT7lacAvailable sinceAug. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRL_GI_PCB28
Plasmid#98745PurposeFor phycocyanobilin production in Saccharomyces cerevisiae. Contains integrative cassette for the yeast his1-Δ200 locus. Encodes constitutively expressed HY1 and PcyA.DepositorInsertsUseSynthetic BiologyTagsExpressionYeastMutationCodon optimized for Saccharomyces cerevisiaePromoterADH1m and PGK1Available sinceAug. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAP-BAC
Plasmid#120229PurposeTAR cloning in yeast and low-copy maintenance of large constructs in E. coliDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKJ217
Plasmid#212318PurposeExpress MmFnuc with pureexpressDepositorInsertMercenaria
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET24a MDHM1_ARATH
Plasmid#204236PurposeBacterial expression of Arabidopsis mitochondrial malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).InsertMDHM1_ARATH (mMDH1 Mustard Weed)
UseTags6X HisExpressionBacterialMutationDoes done include the first 52 residues that are …PromoterLacAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET24a MDHNP_ARATH
Plasmid#204238PurposeBacterial expression of Arabidopsis chloroplastic malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).InsertMDHNP_ARATH (AT5G58330 Mustard Weed)
UseTags6X HisExpressionBacterialMutationDoes not include the chloroplastic transit sequen…PromoterLacAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHCKan-yibDp-ald*-alaE
Plasmid#134939PurposeLow phosphate inducible alanine dehydrogenase and alanine exporterDepositorInsertalanine dehydrogenase and L-alanine exporter
UseTagsExpressionBacterialMutationPromoteryibDAvailable sinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVP91A-pcaHG
Plasmid#113766PurposeExpresses the alpha (pcaG) and the beta (pcaH) subunits of Protocatechuate 3,4-Dioxygenase from Pseudomonas putida.DepositorInsertsβ subunit of Protocatechuate 3,4- Dioxygenase
α subunit of Protocatechuate 3,4- Dioxygenase
UseTagsMet-6xHisExpressionBacterialMutationPromoterT5 promoterAvailable sinceAug. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a hMDH2
Plasmid#204230PurposeBacterial expression of mitochondrial human malate dehydrogenase 2 (hMDH2) transcript variant 1; Use in CURES (integration of research into undergraduate teaching labs).InserthMDH2 (MDH2 Human)
UseTags6X HisExpressionBacterialMutationDoes not include transit peptide and includes a M…PromoterLacAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET30-SBL1(160S,HisTagDel)
Plasmid#66880PurposeExpresses a version of pET30Xa-SBL1(160S) with 41 bp Histag removedDepositorInsertsoybean lipoxygenase-1 (S160S)
UseTagsnoneExpressionBacterialMutationnonePromoterT7lacAvailable sinceAug. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET30-SBL1(160E,HisTagDel)
Plasmid#66879PurposeExpresses a version of pET30Xa-SBL1(160E) with 41 bp Histag removedDepositorInsertsoybean lipoxygenase-1 (S160E)
UseTagsExpressionBacterialMutationS160EPromoterT7lacAvailable sinceAug. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSMART-Ala1
Plasmid#65814PurposeNADPH dependent Alanine Dehydrogenase, Low Phosphate Dependent E. coli ExpressionDepositorInsertNADPH Alanine Dehydrogenase (ald Synthetic)
UseTagsExpressionBacterialMutationD196A, L197RPromoterInsulated waaHp from E. coliAvailable sinceJuly 24, 2015AvailabilityAcademic Institutions and Nonprofits only