Addgene

We narrowed to 991 results for: Hexa

Showing: 121 - 140 of 991 results

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ClCry4 PHR

Plasmid

#160552

Purpose

Expression of protein for various experiments including finding interaction partners

Depositor

Joseph Takahashi

Article

Zoltowski et al Proc Natl Acad Sci U S A. 2019 Sep 24;11

Insert

Clcry4 HR

Use

Tags

Hexa his

Expression

Insect

Mutation

Promoter

Available since

Nov. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pPP381

Plasmid

#158798

Purpose

pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged dCasΦ-2(D394A) in MCS1 for expression in E. coli and protein purification

Depositor

Jennifer Doudna

Article

Pausch et al Science. 2020 Jul 17;369(6501):333-337.

Insert

dCasPhi-2

Use

Tags

6xHis

Expression

Bacterial

Mutation

RuvC-inactivating mutation D394A

Promoter

Available since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pDC158

Plasmid

#115793

Purpose

Expresses Rat SNAP25

Depositor

Axel Brunger

Article

Lai et al Neuron. 2017 Aug 2;95(3):591-607.e10. do

Insert

SNAP25 (Snap25 Rat)

Use

Tags

hexa histidine

Expression

Bacterial

Mutation

c84s, c85s, c90s, c92s

Promoter

T7

Available since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pDC191

Plasmid

#115792

Purpose

Expresses Rat synaptobrevin 2

Depositor

Axel Brunger

Article

Lai et al Neuron. 2017 Aug 2;95(3):591-607.e10. do

Insert

Synaptobrevin2 (Vamp2 Rat)

Use

Tags

hexa histidine

Expression

Bacterial

Mutation

S28C and C103S (please see comments below)

Promoter

T7

Available since

Oct. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pBN095

Plasmid

#173765

Purpose

express E. coli E69 (O9a:K30) Wzc with periplasmic motif 3 truncation

Depositor

James Naismith

Article

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc with periplasmic motif 3 truncation

Use

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

delete motif 3

Promoter

araBAD

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBN215

Plasmid

#167962

Purpose

express E. coli E69 (O9a:K30) Wzc with Y715E, Y717E, Y718E

Depositor

James Naismith

Article

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc with Y715E, Y717E, Y718E

Use

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

Y715E, Y717E, Y718E

Promoter

araBAD

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBN216

Plasmid

#167963

Purpose

express E. coli E69 (O9a:K30) Wzc with Y713E, Y715E, Y717E, Y718E

Depositor

James Naismith

Article

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc with Y713E, Y715E, Y717E, Y718E

Use

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

Y713E, Y715E, Y717E, Y718E

Promoter

araBAD

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBN217

Plasmid

#167964

Purpose

express E. coli E69 (O9a:K30) Wzc with Y708E, Y713E, Y715E, Y717E, Y718E

Depositor

James Naismith

Article

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc with Y708E, Y713E, Y715E, Y717E, Y718E

Use

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

Y708E, Y713E, Y715E, Y717E, Y718E

Promoter

araBAD

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBN261

Plasmid

#167966

Purpose

express E. coli E69 (O9a:K30) Wzc with Y713E, Y715E, Y717E

Depositor

James Naismith

Article

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc with Y713E, Y715E, Y717E

Use

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

Y713E, Y715E, Y717E

Promoter

araBAD

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBN262

Plasmid

#167967

Purpose

express E. coli E69 (O9a:K30) Wzc with Y708E, Y713E, Y715E, Y717E

Depositor

James Naismith

Article

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc with Y708E, Y713E, Y715E, Y717E

Use

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

Y708E, Y713E, Y715E, Y717E

Promoter

araBAD

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBRC901

Plasmid

#167948

Purpose

express wild-type bacterial tyrosine kinase Wzc from E. coli E69 (O9a:K30)

Depositor

James Naismith

Article

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

wild-type bacterial tyrosine kinase Wzc from E. coli E69 (O9a:K30)

Use

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

Promoter

araBAD

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBN008

Plasmid

#167949

Purpose

express E. coli E69 (O9a:K30) Wzc with K540M mutation

Depositor

James Naismith

Article

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

bacterial tyrosine kinase Wzc from E. coli E69 (O9a:K30), K540M nutant

Use

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

K540M

Promoter

araBAD

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBN202

Plasmid

#167950

Purpose

express E. coli E69 (O9a:K30) Wzc_K540M with Y713E, Y715E, Y717E, Y718E mutations

Depositor

James Naismith

Article

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc_K540M with Y713E, Y715E, Y717E, Y718E mutations

Use

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

K540M, Y713E, Y715E, Y717E, Y718E

Promoter

araBAD

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBN093

Plasmid

#167951

Purpose

express E. coli E69 (O9a:K30) Wzc_K540M with periplasmic motif 3 truncation

Depositor

James Naismith

Article

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc_K540M with periplasmic motif 3 truncation

Use

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

K540M, delete motif 3

Promoter

araBAD

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-PTBP1CCtoSS

Plasmid

#89157

Purpose

Recombinant expression of PTBP1 C250S,C251S in E.coli

Depositor

Frédéric Allain

Article

Dorn et al Nat Methods. 2017 Mar 27. doi: 10.1038/n

Insert

Polypyrimidine Tract Binding Protein 1 mutant Cys250Ser, Cys251Ser (PTBP1 Human)

Use

Tags

hexa His

Expression

Bacterial

Mutation

mutations: Cys 250 to Ser, Cys 251 to Ser

Promoter

T7

Available since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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pBAD mApple

Plasmid

#91772

Purpose

Bacterial expression of a fluorescent protein, monomeric Apple (mApple). Note: Addgene identified several mutations compared to the final published sequence.

Depositor

Roger Tsien, Erik Rodriguez

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

monomeric Apple

Use

Tags

Hexa-Histidine tag, Xpress epitope for detection …

Expression

Bacterial

Mutation

R97K, E152S, A180S, V207T compared to mApple (FP …

Promoter

araBAD

Available since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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pSCrhaB2_ApyD_ RBS_ApyAOHIS

Plasmid

#226247

Purpose

Production of ApyA peptide modified by the B12-rSAM enzyme ApyD (optional, refer to publication), the cytochrome P450 enzyme ApyO, and the MNIO enzyme ApyHI, and the methyltransferase ApyS

Depositor

Wilfred van der Donk

Article

Nguyen et al ACS Cent Sci. 2024 Apr 11;10(5):1022-103

Inserts

His6_ApyA
ApyD
ApyO
ApyH
ApyI
ApyS

Use

Tags

Hexa-Histidine

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pSCrhaB2_ApyOAHIDS

Plasmid

#226246

Purpose

Production of ApyA peptide modified by the B12-rSAM enzyme ApyD (optional, refer to publication), the cytochrome P450 enzyme ApyO, and the MNIO enzyme ApyHI, and the methyltransferase ApyS

Depositor

Wilfred van der Donk

Article

Nguyen et al ACS Cent Sci. 2024 Apr 11;10(5):1022-103

Inserts

His6_ApyA
ApyD
ApyO
ApyH
ApyI
ApyS

Use

Tags

Hexa-Histidine

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pSCrhaB2_ApyOAHID

Plasmid

#226245

Purpose

Production of ApyA peptide modified by the B12-rSAM enzyme ApyD (optional, refer to publication), the cytochrome P450 enzyme ApyO, and the MNIO enzyme ApyHI

Depositor

Wilfred van der Donk

Article

Nguyen et al ACS Cent Sci. 2024 Apr 11;10(5):1022-103

Inserts

His6_ApyA
ApyD
ApyO
ApyH
ApyI

Use

Tags

Hexa-Histidine

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pNCS mCherry

Plasmid

#91769

Purpose

Bacterial expression of a fluorescent protein, monomeric Cherry (mCherry)

Depositor

Erik Rodriguez, Roger Tsien

Article

Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

Insert

monomeric Cherry

Use

Tags

Hexa-Histidine tag, Xpress epitope for detection …

Expression

Bacterial

Mutation

See Depositor Comments

Promoter

T7

Available since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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