-
Plasmid#103417PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-30d-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-30d-5p target (MIR30D Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pExp_CAG:EBFP2
Plasmid#51790PurposeConstitutively expresses blue fluorescent protein, EBFP2DepositorInsertEBFP2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLV_TRET_hNgn1_UBC_Bla
Plasmid#61473Purpose3rd generation lentiviral vector; TetON promoter driving human Ngn1; constitutive expression of Blasticidine selection markerDepositorInsertsUseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-21-5p
Plasmid#103345PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-21-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-21-5p target (MIR21 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-132-3p
Plasmid#103219PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-132-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-132-3p target (MIR132 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
GST-PS-Grb2 1-154 (XSB382)
Plasmid#78510PurposeConstruct for expressing Grb2 lacking the C-terminal SH3 domain in E. ColiDepositorInsertGrb2 1-154 (GRB2 Human)
UseTagsGST followed by a prescission protease claevage s…ExpressionBacterialMutationPromoterAvailable sinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMT GFP
Plasmid#126657PurposeInducible expression of GFP in S2 cellsDepositorInsertGFP
UseTagsExpressionInsectMutationPromoterAvailable sinceDec. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFasBac+GFP-CAMSAP1
Plasmid#59036Purposefor baculovirus expression of CAMSAP1 in Sf9 cellsDepositorInsertCAMSAP1 (CAMSAP1 Human)
UseTagsHis and eGFPExpressionInsectMutationPromoterAvailable sinceSept. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAc-mCh-Tub
Plasmid#24288DepositorInsertAlpha-Tubulin (alphaTub84B Fly)
UseTagsmCherryExpressionInsectMutationPromoterAvailable sinceApril 23, 2010AvailabilityAcademic Institutions and Nonprofits only -
pMT-Aurora B-GFP
Plasmid#24285DepositorInsertAurora B Kinase (aurB Fly)
UseTagsGFPExpressionInsectMutationPromoterAvailable sinceMay 10, 2010AvailabilityAcademic Institutions and Nonprofits only -
pHR-DHFRY100I-sfGFP-NLS-P2A-NLS-mCherry-P2A_ control UTRs
Plasmid#67929PurposeTranslational reporter - control UTRsDepositorInsertsfGFP-NLS-P2A-NLS-mCherry
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFasBac+GFP-CAMSAP3
Plasmid#59038Purposefor baculovirus expression of mouse CAMSAP3 in Sf9 cellsDepositorInsertCAMSAP3 (Camsap3 Mouse)
UseTagsHis and eGFPExpressionInsectMutationPromoterAvailable sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET30b-5M SrtA with TEV site
Plasmid#86962PurposeExpresses calcium-dependent Sortase A with a TEV cleavage site proximal to the C-terminal histidine tagDepositorInsertS.aureus SrtA 5M
UseTags6x Histidine tag and TEV siteExpressionBacterialMutationP94R, D160N, D165A, K190E, K196TPromoterT7Available sinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFastBac (GST-SNAP-Shp2)
Plasmid#177897PurposeBaculo expression of GST tag fused to SNAP tag and human Shp2DepositorInsertShp2 (PTPN11 Human)
UseTagsTEV-GST-PreScission cut site-SNAP tagExpressionInsectMutationPromoterPolyhedrinAvailable sinceDec. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-29b-3p
Plasmid#103394PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-29b-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-29b-3p target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
RP hk339-GFP
Plasmid#24431DepositorInsertKinesin WT (KIF5B Human)
UseTags6XHis and GFPExpressionBacterialMutationmonomeric kinesinPromoterAvailable sinceJune 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-27a-3p
Plasmid#103381PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-27a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-27a-3p target (MIR27A Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMT-mCherry-Patronin
Plasmid#31753DepositorInsertPatronin (Patronin Fly)
UseTagsmCherryExpressionInsectMutationPromoterMetallothioneinAvailable sinceSept. 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET28a(ICOS)
Plasmid#177903PurposeBacterial expression of his10-tagged human ICOS Intracellular DomainDepositorInsertICOS (aa 162-199 only) (ICOS Human)
UseTags10xHis-Thrombin cut site-T7 tagExpressionBacterialMutationPromoterT7Available sinceDec. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTK295
Plasmid#59564PurposeSIN SP6 P1234 (nsP2 P726S) SGP(14) 2xK-turn Kozak EGFPDepositorInsertSGP(14) 2xK-turn Kozak EGFP
UseTagsExpressionMutationPromoterAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only