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Showing: 121 - 140 of 1261 results

  7. pQE80L-SpyCatcher-ELP-GFP

    Plasmid
    #69835
    Purpose
    Bacterial expression plasmid containing SpyCatcher-ELP-GFP fusion. SpyCatcher forms a covalent bond with SpyTag and can be used to label plasma membrane localized SpyTag-C1C2 in live cells.
    Depositor
    Viviana Gradinaru
    Article
    Bedbrook et al Chem Biol. 2015 Aug 20;22(8):1108-21. do
    Insert
    SpyCatcher-ELP-GFP
    Use
    Tags
    6x His Tag, GFP, and TEV Tag
    Expression
    Bacterial
    Mutation
    Promoter
    T5 promoter/lac operator element
    Available since
    July 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    69835-plasmid-map-sequence-id-183106

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  13. pSG66-KcsA-Kv1.3-sfGFP

    Plasmid
    #102457
    Purpose
    Expresses a fusion protein of KcsA-Kv1.3 and sfGFP with a C-terminal His tag. The expression is controlled by a OR2-OR1-Pr promoter.
    Depositor
    Richard Murray
    Article
    Guo et al bioRxiv 104455
    Insert
    KcsA-Kv1.3
    Use
    Tags
    His tag and sfGFP
    Expression
    Bacterial
    Mutation
    Promoter
    OR2-OR1-Pr
    Available since
    Oct. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    102457-plasmid-map-sequence-id-197242

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  14. Venus-7aa-MAO-pEGFP-C3

    Plasmid
    #166765
    Purpose
    To target Venus to the mitochondrial outer membrane. MAO tail anchor inserts into the outer membrane and Venus faces the cytosol. Serves as a collisional control in FLIM-FRET experiments.
    Depositor
    David Andrews
    Insert
    MAO tail anchor (MAOA Human)
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    166765-plasmid-map-sequence-id-328859

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  15. pSG69-KcsA-Kv1.3-deGFP

    Plasmid
    #102458
    Purpose
    Expresses KcsA-Kv1.3 protein with a C-terminal His tag and a deGFP fluorescent protein with a different ribosome binding site.
    Depositor
    Richard Murray
    Article
    Guo et al bioRxiv 104455
    Inserts
    KcsA-Kv1.3
    deGFP
    Use
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    Promoter
    OR2-OR1-Pr
    Available since
    Oct. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    102458-plasmid-map-sequence-id-197246

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  18. pSG74-β2AR-sfGFP

    Plasmid
    #102460
    Purpose
    Expresses a fusion protein of wild type β2 adrenergic receptor and sfGFP with a C-terminal His tag. The expression is controlled by a OR2-OR1-Pr promoter.
    Depositor
    Richard Murray
    Article
    Guo et al bioRxiv 104455
    Insert
    β2AR
    Use
    Tags
    His tag and sfGFP
    Expression
    Bacterial
    Mutation
    Promoter
    OR2-OR1-Pr
    Available since
    Oct. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    102460-plasmid-map-sequence-id-197250

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  20. pSG73-β1ARts-sfGFP

    Plasmid
    #102459
    Purpose
    Expresses a fusion protein of thermostabilized β1 adrenergic receptor and sfGFP with a C-terminal His tag. The expression is controlled by a OR2-OR1-Pr promoter.
    Depositor
    Richard Murray
    Article
    Guo et al bioRxiv 104455
    Insert
    β1AR
    Use
    Tags
    His tag and sfGFP
    Expression
    Bacterial
    Mutation
    Promoter
    OR2-OR1-Pr
    Available since
    Oct. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    102459-plasmid-map-sequence-id-200297

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Showing: 121 - 140 of 1261 results