Showing: 121 - 140 of 1496 results
-
Plasmid#198175PurposeExpression of GAL4 transcriptional activation domain (AD)-AP-3 Ī¼3B fusion protein in yeast (yeast two-hybrid assays)DepositorInsertAP-3 Ī¼3B
UseTagsGAL4 transcriptional activation domain (AD) fragmā¦ExpressionYeastMutationPromoterADH1AvailabilityAcademic Institutions and Nonprofits only -
pGBT9-GGA2-GAE
Plasmid#199175PurposeExpression of GAL4 DNA-binding domain (BD)-GGA2-GAE domain fusion protein in yeast (yeast two-hybrid assays)DepositorInsertGGA2-GAE domain (GGA2 Human)
UseTagsGAL4-DNA binding domain fragmentExpressionYeastMutationContains Lys 545 Gln substitutionPromoterADH1AvailabilityAcademic Institutions and Nonprofits only -
VSVG-mCherry-RUSH
Plasmid#203647PurposeRUSH cargo: Str-KDEL_IRES_SBP-mCherry-VSVGDepositorInsertVSVG
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
Lamp1-mCherry-RUSH
Plasmid#202799PurposeRUSH cargo: Str-KDEL_IRES_SBP-mCherry-Lamp1DepositorInsertLamp1 (Lamp1 Rat)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pEGFPN1-ATG9A
Plasmid#198529PurposeExpression of ATG9A-GFP fusion protein in mammalian cellsDepositorInsertATG9A (ATG9A Human)
UseTagsEGFPExpressionMammalianMutationsilent mutations in codons 4 and 775PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pCI-neo-(HA)3-Īµ
Plasmid#198531PurposeExpression of HA-tagged AP-4 Īµ in mammalian cellsDepositorInsertAP-4 Īµ (AP4E1 Human)
UseTagstriple HA tagExpressionMammalianMutationsilent substitutions in codons 235, 262, 905 and ā¦PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pTRIPZ-shWT1_A
Plasmid#217636PurposeDox inducible shRNA targeting WT-1DepositorInsertshWT1_A (WT1 Human)
UseRNAiTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pTRIPZ-shWT1_B
Plasmid#217637PurposeDox inducible shRNA targeting WT-1DepositorInsertshWT1_B (WT1 Human)
UseRNAiTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pMXspuro-GFP-NBR1-dUBA
Plasmid#74205PurposeRetroviral expression of GFP-NBR1 with aa 877 mutation to generate a premature stop codonDepositorInsertneighbor of BRCA1 gene 1 (NBR1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationChanged aa 877 to encode for premature stop codonPromoterAvailabilityAcademic Institutions and Nonprofits only -
pOGG043
Plasmid#133123PurposeLevel 0 PU module synthesised as a fragment (pL0M-PU-PsNifH). Synthetic consensus promoter to drive nodule-specific expression in biovars and strains of R. leguminosarum.DepositorInsertPsNifH Synthetic consensus nifH promoter
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCI-Myc3-HPS4
Plasmid#133931PurposeExpresses 3xMyc-HPS4 construct in mammalian cellsDepositorInsertHPS4 (HPS4 Human)
UseTags3xMyc tagExpressionMammalianMutationGlutamic acid 229 to Glycine; Valine 552 to Methiā¦PromoterCMV I.E.AvailabilityAcademic Institutions and Nonprofits only -
pSEP-LAMP1-RFP
Plasmid#167970PurposeExpresses the chimeric construct SEP-LAMP1-RFP where superecliptic pHluorin (SEP) is fused to the luminal domain of LAMP1-RFPDepositorInsertLAMP1 (Lamp1 Rat)
UseTagsSEP and mRFPExpressionMammalianMutationD50EPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
mCh-BICD2*-strep
Plasmid#120168PurposeExpresses a chimera of mCherry (fluorescent tag), a BICD2-based adaptor and streptavidinDepositorInsertBICD cargo adaptor 2 (Bicd2 Mouse)
UseTagsStreptavidin and mCherryExpressionMammalianMutationTruncated BICD2: 15-595 aaPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
HA-KIF5B*-strep
Plasmid#120170PurposeExpresses a chimera of HA tag, a KIF5B-based anterograde motor and streptavidinDepositorInsertkinesin family member 5B (Kif5b Mouse)
UseTagsHA and StreptavidinExpressionMammalianMutationTruncated KIF5B: 1-555PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
Strep-KIFC1*-HA
Plasmid#120171PurposeExpresses a chimera of streptavidin, a KIFC1-based retrograde motor and HA tagDepositorInsertkinesin family member C1 (KIFC1 Human)
UseTagsHA and StreptavidinExpressionMammalianMutationTruncated KIFC1: 125-673 aaPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
LAMP1-SBP-GFP
Plasmid#120172PurposeExpresses a chimera of LAMP1, SBP tag and GFP (fluorescent tag)DepositorInsertLysosomal-associated membrane protein 1 (Lamp1 Rat)
UseTagsSBP and eGFPExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
eGFP-KIF5A-Rigor
Plasmid#172202PurposeExpresses KIF5A G235A rigor mutant tagged with eGFP in mammalian cellsDepositorInsertKIF5A (Kif5a Mouse)
UseTagseGFPExpressionMammalianMutationChanged Glycine 235 to AlaninePromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
eGFP-KIF1A-Rigor
Plasmid#172207PurposeExpresses KIF1A G251A rigor mutant tagged with eGFP in mammalian cellsDepositorInsertKIF1A (Kif1a Mouse)
UseTagseGFPExpressionMammalianMutationChanged Glycine 251 to AlaninePromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pEF6 VPS51-V5-6His
Plasmid#175502PurposeV5 tagged VPS51 is expressed in mammalian cellsDepositorInsertVPS51 (VPS51 Human)
UseTagsV5ExpressionMammalianMutationA661V- please see depositor commentsPromoterAvailabilityAcademic Institutions and Nonprofits only -
GST-GGA3-VHS-GAT
Plasmid#173719PurposeVHS-GAT domain in GGA3 can bind to an active form of ARF1 protein. Using this construct, you can assess the activity of ARF1 protein.DepositorInsertGGA3
UseTagsGSTExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 121 - 140 of 1496 results