We narrowed to 6,363 results for: wus

1. pcDNA 3.1 LIC 6A NLRP1-FLAG E1322R

   Plasmid

   #166809

   Purpose

   Mammalian cell expression vector for FLAG-tagged NLRP1. The E1322R mutant abolishes interface III UPA-UPA contacts, DPP9 binding, and inflammasome activity

   Depositor

   Hao Wu

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   E1322R

   Promoter

   CMV

   Available Since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV-SARS-CoV-2 SL1 alone-Luciferase

   Plasmid

   #191482

   Purpose

   Luciferase reporter for SARS-CoV-2 5' UTR SL1 alone

   Depositor

   Hao Wu

   Article

   Shi et al bioRxiv. 2020 Sep 18. doi: 10.1101/2020.

   Insert

   SARS-CoV-2 5' UTR SL1 alone
   

   Use

   Lentiviral and Luciferase

   Tags

   Expression

   Mutation

   Contains only the stem-loop1 region of SARS-CoV-2…

   Promoter

   Available Since

   Nov. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFastBac-HS-NLRC4Δcard

   Plasmid

   #177276

   Purpose

   Purification of mouse NLRC4 (90-1024) from insect cells

   Depositor

   Hao Wu

   Article

   Zhang et al Science. 2015 Oct 23;350(6259):404-9. do

   Insert

   NLRC4 (Nlrc4 Mouse)
   

   Use

   Tags

   HIS-SUMO-PreScission

   Expression

   Insect

   Mutation

   I586T, N761S, M885V, K915R and F972L- please see …

   Promoter

   polyhedrin

   Available Since

   Jan. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1 LIC 6A FLAG-TEV-DPP9S S730A

   Plasmid

   #166801

   Purpose

   Mammalian cell expression vector for FLAG-tagged DPP9S. The S730A mutant is catalytically dead.

   Depositor

   Hao Wu

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   DPP9 (DPP9 Human)
   

   Use

   Tags

   FLAG-TEV

   Expression

   Mammalian

   Mutation

   S730A

   Promoter

   CMV

   Available Since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. TRBV12-4

   Plasmid

   #123446

   Purpose

   construction of TCR

   Depositor

   Catherine Wu

   Article

   Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

   Insert

   TRBV12-4 (TRBV12-4 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   Available Since

   May 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. TRAV2

   Plasmid

   #123369

   Purpose

   construction of TCR

   Depositor

   Catherine Wu

   Article

   Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

   Insert

   TRAV2 (TRAV2 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   Available Since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET29a-hNABP2-N

   Plasmid

   #128308

   Purpose

   Express hNABP2 N-terminus (1–138 aa) in E. coli with a C-terminal His-tag

   Depositor

   Yuliang Wu

   Article

   Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

   Insert

   hNABP2 N terminal fragment (OB fold) (NABP2 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   N-terminal domain (1–138 aa)

   Promoter

   T7

   Available Since

   Oct. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET29a-hNABP2NhNABP1C

   Plasmid

   #128313

   Purpose

   Express chimeric protein hNABP2 N-terminus and hNABP1 C-terminus in E. coli with a C-terminal His-tag

   Depositor

   Yuliang Wu

   Article

   Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

   Insert

   chimeric protein hNABP2N+hNABP1C (NABP2, NABP1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   chimeric protein hNABP2N+hNABP1C, Y85C (please se…

   Promoter

   T7

   Available Since

   Aug. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDB.His.MBP.3C NLRP1 UPA-CARD WT

   Plasmid

   #164003

   Purpose

   Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged NLRP1 UPA-CARD

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Tags

   His6-MBP-3C

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1 LIC 6A FLAG-TEV-DPP9S KK93EE

    Plasmid

    #166796

    Purpose

    Mammalian cell expression vector for FLAG-tagged DPP9S. KK93EE mutant is partial loss of CARD8 binding.

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    DPP9 (DPP9 Human)
    

    Use

    Tags

    FLAG-TEV

    Expression

    Mammalian

    Mutation

    KK93EE

    Promoter

    CMV

    Available Since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1 LIC 6A FLAG-TEV-DPP9S E597R

    Plasmid

    #166800

    Purpose

    Mammalian cell expression vector for FLAG-tagged DPP9S. E597R mutant is partial loss of CARD8 binding.

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    DPP9 (DPP9 Human)
    

    Use

    Tags

    FLAG-TEV

    Expression

    Mammalian

    Mutation

    E597R

    Promoter

    CMV

    Available Since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDB.His.MBP.3C NLRP1 UPA WT

    Plasmid

    #164010

    Purpose

    Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged NLRP1 UPA

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Tags

    His6-MBP-3C

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. TRBV16

    Plasmid

    #123451

    Purpose

    construction of TCR

    Depositor

    Catherine Wu

    Article

    Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

    Insert

    TRBV16 (TRBV16 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    Available Since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGL3Basic-Mdm3-T5

    Plasmid

    #32373

    Depositor

    Hong Wu

    Article

    Chang et al J Biol Chem. 2004 Jul 9;279(28):29841-8.

    Insert

    Mdm2 promoter (Mdm2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

15. pZP05

    Plasmid

    #232319

    Purpose

    pZP05 was constructed by cloning the oriFn-repA fragment from pCWU6 into the smaller suicide plasmid pCM-galK, facilitating replication in Fusobacterium nucleatum

    Depositor

    Chenggang Wu

    Insert

    Ori-repA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCWU50

    Plasmid

    #232318

    Purpose

    A conditional plasmid system where RepA, required for pCWU6-based plasmid replication, is regulated by an inducible fdx promoter and theophylline-responsive riboswitch

    Depositor

    Chenggang Wu

    Insert

    Pfdx-E
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    fdx

    Available Since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDFDuet-AtUBC8-S

    Plasmid

    #222713

    Purpose

    Expression AtUBC8-S in in bacterial cells

    Depositor

    Lei Wu

    Article

    Pu et al Biomolecules. 2024 Sep 25;14(10):1209. d

    Insert

    AtUBC8 (UBC8 Mustard Weed)
    

    Use

    Tags

    S-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pACYCDuet-AtTIR1-Myc-MBP-AtIAA6-HA

    Plasmid

    #222708

    Purpose

    Expression AtTIR1-Myc and MBP-AtIAA6-HA in in bacterial cells

    Depositor

    Lei Wu

    Article

    Pu et al Biomolecules. 2024 Sep 25;14(10):1209. d

    Inserts

    AtTIR1 (TIR1 Mustard Weed)
    AtIAA6 (IAA6 Mustard Weed)
    

    Use

    Tags

    HA, MBP, and myc

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pACYCDuet-AtTIR1-Myc-MBP-HA

    Plasmid

    #222709

    Purpose

    Expression AtTIR1-Myc and MBP-HA in in bacterial cells

    Depositor

    Lei Wu

    Article

    Pu et al Biomolecules. 2024 Sep 25;14(10):1209. d

    Insert

    AtTIR1 (TIR1 Mustard Weed)
    

    Use

    Tags

    myc

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pACYCDuet-Myc-MBP-AtIAA6-HA

    Plasmid

    #222710

    Purpose

    Expression MBP-AtIAA6-HA in in bacterial cells

    Depositor

    Lei Wu

    Article

    Pu et al Biomolecules. 2024 Sep 25;14(10):1209. d

    Insert

    AtIAA6 (IAA6 Mustard Weed)
    

    Use

    Tags

    HA and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge