We narrowed to 1,497 results for: MBP

1. pJSC011 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), HNH FRET variant

   Plasmid

   #101229

   Purpose

   Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), HNH FRET variant

   Depositor

   Keith Joung, Jennifer Doudna

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant C80S/C574S/S355C/S867C/N692A/M694A/Q695A/H698A
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   C80S, C574S, S355C, S867C, N692A, M694A, Q695A an…

   Promoter

   T7

   Available since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMAL-c5e-Rubicon RH N821K, T911R, K916R

   Plasmid

   #216707

   Purpose

   Bacterial protein expression of Rubicon RH domain mutant

   Depositor

   James Hurley

   Article

   Tudorica et al J Cell Biol. 2024 Jul 1;223(7):e20230901

   Insert

   Rubicon (RUBCN Human)
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   N821K, T911R, K916R

   Promoter

   Available since

   Nov. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. hzHDAC10

   Plasmid

   #225542

   Purpose

   Expresses histone deacetylase 10 (HDAC10) humanized catalytic domain, also known as hzHDAC10, in Escherichia coli cells.

   Depositor

   David Christianson

   Article

   Herbst-Gervasoni et al ACS Chem Biol. 2020 Aug 21;15(8):2154-21

   Insert

   Histone Deacetylase 10 humanized catalytic domain (hdac10 Zebrafish)
   

   Use

   Tags

   His6-MBP tag with cleavable TEV site

   Expression

   Bacterial

   Mutation

   Mutated A24E and D94A

   Promoter

   T7

   Available since

   Oct. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-FLAG-B-eSpCas9

   Plasmid

   #126772

   Purpose

   Expression of increased fidelity Blackjack-eSpCas9 in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than that of eSpCas9

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   B-eSpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG, 6xHis, MBP, and NLS

   Expression

   Bacterial

   Mutation

   K848A; K1003A; R1060A; amino acids 1005-1013 repl…

   Promoter

   T7

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMAL c2x Tral FL-15A

   Plasmid

   #113011

   Purpose

   For bacterial expression of MBP fusion of full-length Drosophila Tral with phosphomutations (15A)

   Depositor

   Terry Orr-Weaver

   Article

   Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

   Insert

   full length tral (tral Fly)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   15 PNG phosphorylation sites mutated to A

   Promoter

   Available since

   Aug. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMAL c2x Tral C 15A

   Plasmid

   #113007

   Purpose

   For bacterial expression of MBP fusion of C terminal region of Drosophila Tral phosphomutant (15A)

   Depositor

   Terry Orr-Weaver

   Article

   Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

   Insert

   C terminus of tral (tral Fly)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   PNG phosphorylation sites mutated to A

   Promoter

   Available since

   Aug. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMAL c2x Tral FL-15D

   Plasmid

   #113010

   Purpose

   For bacterial expression of MBP fusion of full-length Drosophila Tral with Phos-mimic mutations

   Depositor

   Terry Orr-Weaver

   Article

   Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

   Insert

   full length tral (tral Fly)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   15 PNG phosphorylation sites mutated to D

   Promoter

   Available since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJSC115 - Bacterial expression plasmid for SpCas9-HF1, HNH FRET variant

   Plasmid

   #101210

   Purpose

   Bacterial expression plasmid for SpCas9-HF1, HNH FRET variant

   Depositor

   Keith Joung, Jennifer Doudna

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant C80S/C574S/S355C/S867C/N497A/R661A/Q695A/Q926A
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   C80S, C574S, S355C, S867C, N497A, R661A, Q695A an…

   Promoter

   T7

   Available since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJSC038 - Bacterial expression plasmid for SpCas9∆REC3, HNH FRET variant

   Plasmid

   #101203

   Purpose

   Bacterial expression plasmid for SpCas9∆REC3, HNH FRET variant

   Depositor

   Keith Joung, Jennifer Doudna

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant C80S/C574S/S355C/S867C/M1–N497,GGS,V713–D1368
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   C80S, C574S, S355C, S867C, M1-N497, GGS, V713-D13…

   Promoter

   T7

   Available since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJSC131 - Bacterial expression plasmid for SpCas9-HF1, REC3 FRET variant

    Plasmid

    #101212

    Purpose

    Bacterial expression plasmid for SpCas9-HF1, REC3 FRET variant

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant C80S/C574S/S701C/S960C/N497A/R661A/Q695A/Q926A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    C80S, C574S, S701C, S960C, N497A, R661A, Q695A an…

    Promoter

    T7

    Available since

    Oct. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMAL-PIAL2M-wt

    Plasmid

    #67909

    Purpose

    Inducible expression of PIAL2 in E.coli

    Depositor

    Andreas Bachmair

    Article

    Tomanov et al Plant Cell. 2014 Nov;26(11):4547-60. doi

    Insert

    PIAL2 (AT5G41580 Mustard Weed)
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    E281-A496

    Promoter

    P-lac

    Available since

    Aug. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

12. Kv7.4 AD (325-645, C643A, Δ 368-492)/pET28HMT

    Plasmid

    #111072

    Purpose

    Protein expression in E.Coli

    Depositor

    Dan Minor

    Article

    Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

    Insert

    potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
    

    Use

    Tags

    His Tag and MBP tag

    Expression

    Bacterial

    Mutation

    Kv7.4 AD (325-645, C643A, Δ 368-492)

    Promoter

    T7

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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13. Kv7.4 BD (522-645, C643A)/pET28HMT

    Plasmid

    #111073

    Purpose

    Protein expression in E.Coli

    Depositor

    Dan Minor

    Article

    Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

    Insert

    potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
    

    Use

    Tags

    His Tag and MBP tag

    Expression

    Bacterial

    Mutation

    Kv7.4 BD (522-645, C643A)

    Promoter

    T7

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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14. Kv7.5 AB (361-545, Δ395-511)/pET28HMT

    Plasmid

    #111267

    Purpose

    Protein expression in E.Coli

    Depositor

    Dan Minor

    Article

    Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

    Insert

    potassium voltage-gated channel subfamily KQT member 5 isoform 1 [Homo sapiens] (KCNQ5 Human)
    

    Use

    Tags

    His Tag and MBP Tag

    Expression

    Bacterial

    Mutation

    Kv7.5 AB (361-545, Δ395-511)

    Promoter

    T7

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJ4M/TDP-43

    Plasmid

    #104480

    Purpose

    bacterial expression of TDP-43

    Depositor

    Nicolas Fawzi

    Article

    Wang et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Tags

    MBP-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. TH1204-pOCC120-FUS_opt(NheI)

    Plasmid

    #221892

    Purpose

    Shuttle vector for baculovirus production, using FlashBac bacmid

    Depositor

    Anthony Hyman

    Article

    Wang et al Cell. 2018 Jul 26;174(3):688-699.e16. do

    Insert

    FUS (FUS Human)
    

    Use

    Tags

    MBP-PreScission and mGFP-PreScission-6xHis

    Expression

    Insect

    Mutation

    wt

    Promoter

    PH promoter

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEN017-SpCas9-hiNLS-t-M4

    Plasmid

    #234455

    Purpose

    Expresses hiNLS variant t-M4 inside 0C triNLS-SpCas9-NGG

    Depositor

    Ross Wilson

    Article

    Noel et al CRISPR J. 2025 Apr;8(2):105-119. doi: 10

    Insert

    SpCas9 hiNLS t-M4
    

    Use

    CRISPR

    Tags

    His6, Nucleoplasmin, SV40, and c-Myc

    Expression

    Bacterial

    Mutation

    Cys at positions 80 and 574 have been mutated to …

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEN019-SpCas9-hiNLS-t-M1M4

    Plasmid

    #234456

    Purpose

    Expresses hiNLS variant t-M1M4 inside 0C triNLS-SpCas9-NGG

    Depositor

    Ross Wilson

    Article

    Noel et al CRISPR J. 2025 Apr;8(2):105-119. doi: 10

    Insert

    SpCas9 hiNLS t-M1M4
    

    Use

    CRISPR

    Tags

    His6, Nucleoplasmin, SV40, and c-Myc

    Expression

    Bacterial

    Mutation

    Cys at positions 80 and 574 have been mutated to …

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. ASC-CASP1 Octamer

    Plasmid

    #164032

    Purpose

    Bacterial expression vector encoding a 5GSS-linked ASC(CARD)-CASP1(CARD) construct to express an octamer

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    CASP1, ASC (CASP1, PYCARD Human)
    

    Use

    Tags

    His6-MBP-TEV

    Expression

    Bacterial

    Mutation

    W169G (ASC), G20K (CASP1)

    Promoter

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28a Cdk2ap1CAN

    Plasmid

    #178032

    Purpose

    Expression vector for the purpose of protein purification.

    Depositor

    Lin He

    Article

    Modzelewski et al Cell. 2021 Oct 7. pii: S0092-8674(21)011

    Insert

    Cdk2ap1 (Cdk2ap1 Mouse)
    

    Use

    Nonviral

    Tags

    6xHIS - Thrombin - MBP- TEV-TRS

    Expression

    Bacterial

    Mutation

    Promoter

    T7LacI

    Available since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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