We narrowed to 2,364 results for: PUC19

1. pITGFP3

   Plasmid

   #65452

   Purpose

   pITGFP3 was designed to test the promoter activity using the destabilized yeast enhanced green fluorescent protein (yEGFP-CLN2PEST) as reporter.

   Depositor

   Claudia Vickers

   Article

   Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

   Type

   Empty backbone

   Tags

   yEGFP-CLN2PEST reporter

   Expression

   Yeast

   Available Since

   June 2, 2015

   Availability

   Academic Institutions and Nonprofits only

2. BC1364-mouse U6-sgH2B

   Plasmid

   #164043

   Purpose

   U6-driven sgRNA targeting H2B

   Depositor

   Baohui Chen

   Article

   Chen et al Nat Commun. 2018 Nov 29;9(1):5065. doi:

   Insert

   H2B targeting sgRNA
   

   Use

   CRISPR

   Promoter

   mouse U6

   Available Since

   Jan. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAT9749-dABE

   Plasmid

   #162998

   Purpose

   plasmid expressing ABE with dCas9

   Depositor

   Ervin Welker

   Article

   Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

   Insert

   dABE
   

   Use

   CRISPR

   Tags

   3xFLAG and SV40 NLS

   Expression

   Mammalian

   Promoter

   EF1-a

   Available Since

   Sept. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAT15002-ABEmax

   Plasmid

   #163006

   Purpose

   plasmid expressing ABEmax

   Depositor

   Ervin Welker

   Article

   Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

   Insert

   ABEmax
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Mammalian

   Promoter

   EF1-a

   Available Since

   Sept. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAT15064-eCBE

   Plasmid

   #163009

   Purpose

   plasmid expressing eCBE

   Depositor

   Ervin Welker

   Article

   Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

   Insert

   eCBE
   

   Use

   CRISPR

   Tags

   3xFLAG and SV40 NLS

   Expression

   Mammalian

   Promoter

   EF1-a

   Available Since

   Sept. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAT9676-ABE

   Plasmid

   #162997

   Purpose

   plasmid expressing ABE

   Depositor

   Ervin Welker

   Article

   Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

   Insert

   ABE
   

   Use

   CRISPR

   Tags

   3xFLAG and SV40 NLS

   Expression

   Mammalian

   Promoter

   EF1-a

   Available Since

   Sept. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pML1

   Plasmid

   #108332

   Purpose

   MIDAS Level-1 source vector for cloning of modules (synthetic biology parts)

   Depositor

   Andrew Muscroft-Taylor

   Article

   van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

   Type

   Empty backbone

   Use

   Synthetic Biology

   Available Since

   June 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJAID5VY

   Plasmid

   #165048

   Purpose

   Integrative expression of CUP1-AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1.

   Depositor

   Claudia Vickers

   Article

   Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

   Insert

   P-URA3>KlURA3>T-AgTEF1-P-TEF1>CUP1-AID*>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
   

   Expression

   Yeast

   Mutation

   OsTIR1 is codon-optimised for expression in S. ce…

   Available Since

   March 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJAID8Z104

   Plasmid

   #165056

   Purpose

   Integrative expression of yEGFP-AID*-CUP1 and SKP1-fusing rice auxin receptor gene OsTIR1.

   Depositor

   Claudia Vickers

   Article

   Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

   Insert

   P-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>AID*>CUP1>T-PGK1-P-ACS2> SKP1>OsTIR1opt>T-URA3
   

   Expression

   Yeast

   Mutation

   WT

   Available Since

   March 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pILGFPQ3

    Plasmid

    #83558

    Purpose

    Used to evaluate the expression output of GAL2 promoter with yEGFP used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

    Insert

    GAL2 promoter-yEGFP
    

    Expression

    Yeast

    Promoter

    GAL2

    Available Since

    Dec. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDiySpike2

    Plasmid

    #136949

    Purpose

    Plasmid for the In Vitro transcription of DiySpike2

    Depositor

    Rickard Sandberg

    Article

    Hagemann-Jensen et al Nat Biotechnol. 2020 Jun;38(6):708-714.

    Insert

    DiySpike2
    

    Use

    Synthetic Biology

    Promoter

    T7

    Available Since

    April 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAVS1-NEO-CAG-BFP-pA-GFP-pA

    Plasmid

    #149344

    Purpose

    Vector for targeting the Replace reporter to the AAVS1 locus

    Depositor

    Ralf Kuehn

    Article

    Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

    Insert

    BFP
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGGC001

    Plasmid

    #48824

    Purpose

    Provides WUS as GreenGate coding sequence module.

    Depositor

    Jan Lohmann

    Article

    Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

    Insert

    WUS (WUS Mustard Weed)
    

    Use

    Golden gate compatible cloning vector

    Mutation

    internal BsaI recognition site removed by silent …

    Available Since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pML3

    Plasmid

    #108341

    Purpose

    MIDAS Level-3 destination vector. Used for initiating Level-3 multigene assembly.

    Depositor

    Andrew Muscroft-Taylor

    Article

    van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

    Type

    Empty backbone

    Use

    Synthetic Biology

    Available Since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. SW Mb 64Y68F

    Plasmid

    #34569

    Depositor

    John Olson

    Article

    Hargrove et al J Biol Chem. 1994 Feb 11;269(6):4207-14.

    Insert

    SW Mb 64Y68F
    

    Expression

    Bacterial

    Mutation

    His 64 to Tyr and Val 68 to Phe

    Available Since

    Jan. 12, 2012

    Availability

    Academic Institutions and Nonprofits only

16. CJ41 (U1 snRNA (U1;21d))

    Plasmid

    #17774

    Depositor

    David Bartel

    Article

    Ruby et al Nature. 2007 Jul 5. 448(7149):83-6.

    Insert

    U1 snRNA (U1;21d) (snRNA:U1:21D Fly)
    

    Available Since

    April 15, 2008

    Availability

    Academic Institutions and Nonprofits only

17. pFN1-catP-oriT

    Plasmid

    #244451

    Purpose

    A pFN1-aad9 plasmid carrying an OriT sequence for conjugative transfer into F. nucleatum.

    Depositor

    Xue Liu

    Insert

    CatP
    

    Expression

    Bacterial

    Available Since

    Oct. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFN1-Aad9

    Plasmid

    #244449

    Purpose

    A shuttle plasmid compatible with Fusobacterium nucleatum ATCC10953 or ATCC25586, carrying a spectinomycin resistance marker (aadA gene).

    Depositor

    Xue Liu

    Inserts

    pFn1 Fn ori
    Aad9
    

    Expression

    Bacterial

    Available Since

    Oct. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGGC018

    Plasmid

    #228232

    Purpose

    Level 0 for LhG4 (GreenGate C module)

    Depositor

    Alexis Maizel

    Article

    Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

    Insert

    GR-LhG4
    

    Use

    Synthetic Biology

    Promoter

    -

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSEED-Moontag:HA

    Plasmid

    #219648

    Purpose

    Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in Moontag and HAtag using the SEED/Harvest method

    Depositor

    Markus Affolter

    Article

    Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

    Insert

    MoonTag:HA SEED Cassette
    

    Available Since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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