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pNVL-pvdIJD

Plasmid

#169872

Purpose

Integration vector for deleting pvdIJD locus in P. putida

Depositor

Brian Pfleger

Publication

Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

Type

Empty backbone

Expression

Bacterial

Available since

July 9, 2021

Availability

Academic Institutions and Nonprofits only

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pETM6-At4CL-PhCHS-MsCHI

Plasmid

#73392

Purpose

Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

Depositor

Mattheos Koffas

Publication

Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

Inserts

At4CL
PhCHS
MsCHI

Expression

Bacterial

Promoter

T7

Available since

April 14, 2016

Availability

Academic Institutions and Nonprofits only

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pETM6-Pc4CL-PhCHS-CmCHI

Plasmid

#73397

Purpose

Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

Depositor

Mattheos Koffas

Publication

Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

Inserts

Pc4CL
PhCHS
CmCHI

Expression

Bacterial

Promoter

T7

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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CpcB•PHLS+Cpc

Plasmid

#73335

Purpose

Expresses the PHLS gene as a fusion with the CpcB gene followed by the CmR gene

Depositor

Anastasios Melis

Publication

Formighieri et al Metab Eng. 2015 Nov;32:116-24. doi: 10.1

Insert

cpcB•PHLS-CmR

Promoter

cpc operon

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pDS1U-PKS12

Plasmid

#70150

Purpose

Fusarium graminearum PKS12 encoded on CEN/ARS yeast shuttle vector with DS1U selection marker

Depositor

Morten Sommer

Publication

Rugbjerg et al Metab Eng. 2015 Sep;31:189-97. doi: 10.1

Insert

PKS12 (PKS12 Fusarium graminearum)

Expression

Yeast

Promoter

CUP1

Available since

Dec. 23, 2015

Availability

Academic Institutions and Nonprofits only

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WT1 KI gRNA1

Plasmid

#92312

Purpose

CRISPR-GFP-gRNA for cutting WT1

Depositor

Sean Palecek

Publication

Bao et al Nat Biomed Eng. 2016;1. pii: 0003. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

WT1 (WT1 Human)

Tags

CRISPR GFP

Expression

Mammalian

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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Bxb1-GT_CAG-mRuby2

Plasmid

#229801

Purpose

Bxb1-GT donor plasmid for integrating a transcription unit for constitutive expression of mRuby2

Depositor

Richard Davis

Publication

Blanch-Asensio et al Nat Biomed Eng. 2026 Apr 30. doi: 10.103

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

CAG

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Bxb1-GA_CAG-EGFP

Plasmid

#229799

Purpose

Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of EGFP

Depositor

Richard Davis

Publication

Blanch-Asensio et al Nat Biomed Eng. 2026 Apr 30. doi: 10.103

Insert

EGFP

Use

Synthetic Biology

Expression

Mammalian

Promoter

CAG

Available since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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evoBxb1_IVT

Plasmid

#222341

Purpose

Plasmid for evolved Bxb1 mRNA in vitro transcription template

Depositor

David Liu

Publication

Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

Insert

Codon-optimized evolved Bxb1

Use

Template for in vitro transcription

Available since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-ABE8e-SpCas9-NRRH-P2A-EGFP (RMD51)

Plasmid

#197502

Purpose

CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-NRRH(D10A) and P2A-EGFP

Depositor

Benjamin Kleinstiver

Publication

Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

pCMV-T7-BPNLS-ABE8e-nSpCas9-NRRH-BPNLS-P2A-EGFP

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS and BPNLS-P2A-EGFP

Expression

Mammalian

Mutation

ABE8e mutations in TadA and nSpCas9-NRRH(D10A/I32…

Promoter

CMV and T7

Available since

March 6, 2023

Availability

Academic Institutions and Nonprofits only

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CMV_Npu-BE3.9max C-terminal

Plasmid

#137172

Purpose

Expresses the C-terminal split-intein fragment of BE3.9max from the CMV promoter

Depositor

David Liu

Publication

Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Npu-BE3.9max_C-terminal

Expression

Mammalian

Promoter

CMV

Available since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

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CMV_Npu-BE3.9max N-terminal

Plasmid

#137171

Purpose

Expresses the N-terminal split-intein fragment of BE3.9max from the CMV promoter

Depositor

David Liu

Publication

Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Npu-BE3.9max_N-terminal

Expression

Mammalian

Mutation

Cas9 D10A

Promoter

CMV

Available since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

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Circular-ic-CVB3-GFP

Plasmid

#226260

Purpose

Production of in situ circularizable RNA via IVT

Depositor

Prashant Mali

Publication

Tong et al Nat Biomed Eng. 2024 Aug 26. doi: 10.103

Insert

EGFP

Use

In vitro transcription

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pTS2356_Tier3(PB)-PuroR

Plasmid

#169655

Purpose

Tier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-PuroR-pA-3'ITR)

Depositor

Martin Fussenegger

Publication

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PRPBSA-driven PuroR expression

Expression

Mammalian

Promoter

PRPBSA

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pCK255

Plasmid

#171155

Purpose

Expression of I-SceI gene for genome engineering in Pseudomonas with sacB gene included for counterselection with sucrose on pBBR1-GmR plasmid

Depositor

Jesse Zalatan

Publication

Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

Insert

I-SceI_sacB

Expression

Bacterial

Available since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pGCG26

Plasmid

#82371

Purpose

YFP expressed from pJ23119 in the glpK locus with gentamicin resistance

Depositor

Brian Pfleger

Publication

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

YFP

Promoter

PJ23119

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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CMV_Npu-ABEmax C-terminal

Plasmid

#137174

Purpose

Expresses the C-terminal split-intein fragment of ABEmax from the CMV promoter

Depositor

David Liu

Publication

Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Npu-ABEmax_C-terminal

Expression

Mammalian

Promoter

CMV

Available since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

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CMV_Npu-ABEmax N-terminal

Plasmid

#137173

Purpose

Expresses the N-terminal split-intein fragment of ABEmax from the CMV promoter

Depositor

David Liu

Publication

Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Npu-ABEmax_N-terminal

Expression

Mammalian

Mutation

Cas9 D10A

Promoter

CMV

Available since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pEM7065

Plasmid

#214003

Purpose

PrpsM driven expression of ssrA-tagged mCerulean (LVA)

Depositor

Marc Erhardt

Publication

Delgadillo-Guevara et al J Biol Eng. 2024 Apr 8;18(1):25. doi: 10

Insert

mCerulean-LVA

Expression

Bacterial

Promoter

PrpsM

Available since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pEM9177

Plasmid

#213994

Purpose

PrpsM driven expression of SSAGSAAGS-mGFPmut2

Depositor

Marc Erhardt

Publication

Delgadillo-Guevara et al J Biol Eng. 2024 Apr 8;18(1):25. doi: 10

Insert

SSAGSAAGS-mGFPmut2

Tags

SSAGSAAGS

Expression

Bacterial

Promoter

PrpsM

Available since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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