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CMV_Npu-BE3.9max N-terminal

Plasmid

#137171

Purpose

Expresses the N-terminal split-intein fragment of BE3.9max from the CMV promoter

Depositor

David Liu

Publication

Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

Insert

Npu-BE3.9max_N-terminal

Expression

Mammalian

Mutation

Cas9 D10A

Promoter

CMV

Available since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

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Circular-ic-CVB3-GFP

Plasmid

#226260

Purpose

Production of in situ circularizable RNA via IVT

Depositor

Prashant Mali

Publication

Tong et al Nat Biomed Eng. 2024 Aug 26. doi: 10.103

Insert

EGFP

Use

In vitro transcription

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pCK255

Plasmid

#171155

Purpose

Expression of I-SceI gene for genome engineering in Pseudomonas with sacB gene included for counterselection with sucrose on pBBR1-GmR plasmid

Depositor

Jesse Zalatan

Publication

Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

Insert

I-SceI_sacB

Expression

Bacterial

Available since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pGCG26

Plasmid

#82371

Purpose

YFP expressed from pJ23119 in the glpK locus with gentamicin resistance

Depositor

Brian Pfleger

Publication

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

YFP

Promoter

PJ23119

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pEM7065

Plasmid

#214003

Purpose

PrpsM driven expression of ssrA-tagged mCerulean (LVA)

Depositor

Marc Erhardt

Publication

Delgadillo-Guevara et al J Biol Eng. 2024 Apr 8;18(1):25. doi: 10

Insert

mCerulean-LVA

Expression

Bacterial

Promoter

PrpsM

Available since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pEM9177

Plasmid

#213994

Purpose

PrpsM driven expression of SSAGSAAGS-mGFPmut2

Depositor

Marc Erhardt

Publication

Delgadillo-Guevara et al J Biol Eng. 2024 Apr 8;18(1):25. doi: 10

Insert

SSAGSAAGS-mGFPmut2

Tags

SSAGSAAGS

Expression

Bacterial

Promoter

PrpsM

Available since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pEM7071

Plasmid

#214009

Purpose

PrpsM driven expression of ssrA-tagged mScarlet-I (ASV)

Depositor

Marc Erhardt

Publication

Delgadillo-Guevara et al J Biol Eng. 2024 Apr 8;18(1):25. doi: 10

Insert

mScarlet-I-ASV

Expression

Bacterial

Promoter

PrpsM

Available since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCI-T7Max-UTR1-T500

Plasmid

#178418

Purpose

Empty backbone. T7Max promoter

Depositor

Kate Adamala

Publication

Deich et al J Biol Eng. 2023 Jan 23;17(1):4. doi: 10

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7Max

Available since

Dec. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pMST-712

Plasmid

#118080

Purpose

pmbfA::cexA::ttrpC

Depositor

Michael Sauer

Publication

Steiger et al Metab Eng. 2019 Mar;52:224-231. doi: 10.

Insert

CexA

Promoter

pmbfA

Available since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pMST-1313

Plasmid

#118076

Purpose

pTEF1-cexA-tCYC1

Depositor

Michael Sauer

Publication

Steiger et al Metab Eng. 2019 Mar;52:224-231. doi: 10.

Insert

pTEF1-cexA-tCYC1

Expression

Yeast

Promoter

pTEF1

Available since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pPS3911

Plasmid

#113330

Purpose

control for Hermes transposition; TIR-flanked LEU2, transposase absent

Depositor

Suzanne Sandmeyer

Publication

Patterson et al Metab Eng. 2018 May 22;48:184-196. doi:

Insert

beta-isopropyl malate dehydrogenase

Expression

Yeast

Available since

Aug. 17, 2018

Availability

Academic Institutions and Nonprofits only

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JBEI-9348

Plasmid

#84981

Purpose

Isopentenol production

Depositor

Taek Soon Lee

Publication

Kang et al Metab Eng. 2016 Mar;34:25-35. doi: 10.10

Insert

PMD-NudB

Expression

Bacterial

Promoter

trc promoter

Available since

Aug. 1, 2017

Availability

Academic Institutions and Nonprofits only

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JBEI-9314

Plasmid

#84979

Purpose

Isopentenol production

Depositor

Taek Soon Lee

Publication

Kang et al Metab Eng. 2016 Mar;34:25-35. doi: 10.10

Insert

PMD

Expression

Bacterial

Promoter

trc promoter

Available since

Aug. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pBbB0a-RFP

Plasmid

#35371

Depositor

Jay Keasling

Publication

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Available since

July 23, 2012

Availability

Academic Institutions and Nonprofits only

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pBbB0k-RFP

Plasmid

#35370

Depositor

Jay Keasling

Publication

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Available since

March 7, 2012

Availability

Academic Institutions and Nonprofits only

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pBbS3c-RFP

Plasmid

#35311

Depositor

Jay Keasling

Publication

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

ProS

Available since

Feb. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pBbE3c-RFP

Plasmid

#35305

Depositor

Jay Keasling

Publication

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

ProS

Available since

Feb. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pIVT-mGL

Plasmid

#232768

Purpose

IVT template for making modRNA encoding mGreenLantern

Depositor

Kate Galloway

Publication

Blanch-Asensio et al Nat Biomed Eng. 2026 Apr 30. doi: 10.103

Insert

mGreenLantern

Use

In vitro transcription

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pCHC005 (∆RE)

Plasmid

#226199

Purpose

Conjugation knockout plasmid (pK18mobsacB) to delete H16_0006 (Type I restriction endonuclease subunit).

Depositor

Christopher Johnson

Publication

Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

Insert

Homology arms for type I restriction-modification system restriction subunit H16_A0006

Use

Synthetic Biology

Available since

Nov. 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pCHC042 (∆phaCAB)

Plasmid

#226208

Purpose

Conjugation knockout plasmid (pK18msB) to delete the C. necator phaCAB operon, to prevent accumulation of PHB.

Depositor

Christopher Johnson

Publication

Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

Insert

Homology arms for phaCAB

Use

Synthetic Biology

Available since

Nov. 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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