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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 5,317 results for: golden gate

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Showing: 1381 - 1400 of 5317 results

  4. EC71139

    Plasmid
    #154062
    Purpose
    Level 0 Golden Gate vector, containing the ZmUbi promoter and 5' UTR with Level 0.5-compatible overhangs (Barley Construct)
    Depositor
    Cristobal Uauy
    Publication
    Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:
    Insert
    pZmUbi
    Use
    Synthetic Biology
    Mutation
    All BsaI, Esp3I and BpiI restriction sites were r…
    Available since
    Sept. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    154062-plasmid-map-sequence-id-312952

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  6. pEPOR1CB0113

    Plasmid
    #117552
    Purpose
    Level 1 Golden Gate Cassette: eSaCas9 expression cassette for dicotyledonous plants
    Depositor
    Nicola Patron
    Publication
    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do
    Insert
    2x35S+5'UTR OMEGA (pICH51288) + eSaCas9 (no stop codon) (pEPOR0SP0012) + YFP (pICSL50005) + Nos (pICH41421)
    Expression
    Plant
    Promoter
    2x35S+5'UTR OMEGA (pICH51288)
    Available since
    Nov. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    117552-plasmid-map-sequence-id-228090

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  10. pYPQ136B2.0

    Plasmid
    #167160
    Purpose
    Golden Gate entry vector to express the 6th gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter
    Depositor
    Yiping Qi
    Publication
    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Plant
    Promoter
    AtU3
    Available since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    167160-plasmid-map-sequence-id-331282

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  12. pKEK3238

    Plasmid
    #234092
    Purpose
    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.
    Depositor
    Karl Klose
    Publication
    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000
    Insert
    sfCherry2
    Expression
    Bacterial
    Mutation
    E117Q/T127I/G219A
    Promoter
    pJ23100-BsaI
    Available since
    April 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    234092-plasmid-map-sequence-id-467988

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  16. PB-CT3G-ERP2

    Plasmid
    #175497
    Purpose
    All-in-one piggyBac transposon vector for mCMV+dox-inducible expression of Golden Gate cloned elements (hEF1a-driven rtTA and puromycin resistance)
    Depositor
    George Church
    Publication
    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    TRE3G-mCMV
    Available since
    Oct. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    175497-plasmid-map-sequence-id-345451

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Showing: 1381 - 1400 of 5317 results