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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 5,818 results for: golden gate

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Showing: 1381 - 1400 of 5818 results

  5. sgRNA_expression_vector

    Plasmid
    #210212
    Purpose
    An empty gRNA expression vector to clone U6 promoter driven sgRNAs with gRNA scaffold and with co-expression of DsRed. sgRNA can be cloned by Golden Gate Assembly or restriction digestion using BbsI.
    Depositor
    Albert Jeltsch
    Publication
    Rajaram et al Methods Mol Biol. 2024;2842:179-192. doi
    Type
    Empty backbone
    Expression
    Bacterial and Mammalian
    Promoter
    U6
    Available since
    Dec. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    210212-plasmid-map-sequence-id-416400

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  8. IBMc460

    Plasmid
    #161940
    Purpose
    Carries Golden Gate substitution insert for IBM with gp41-1 intein. PPhlF as C-lobe promoter
    Depositor
    Baojun Wang
    Publication
    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:
    Insert
    pSB1A3-BbsI-GY-gp-41-1N-cat-PPhlF-RiboJ-B32-gp41-1C-SS-SapI
    Expression
    Bacterial
    Promoter
    PPhlF
    Available since
    March 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    161940-plasmid-map-sequence-id-329041

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  13. pNM3749

    Plasmid
    #154108
    Purpose
    loxP FRT FRT3 RMCE landing site in a Golden Gate SapI GCG ACG assembly vector
    Depositor
    Michael Nonet
    Publication
    Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.
    Inserts
    LoxP-SEC-LoxP
    FRT::rpl-28p::GFP::his-58::FRT3
    Use
    Cre/Lox; Rmce
    Expression
    Worm
    Promoter
    not applicable
    Available since
    July 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    154108-plasmid-map-sequence-id-306067

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  15. pGGD_MiniTurboID-YFP

    Plasmid
    #222434
    Purpose
    Golden Gate / Green Gate module for adding MiniTurboID and YFP as C-terminus of target protein.
    Depositor
    Dominique Bergmann
    Publication
    Wallner et al Dev Cell. 2024 May 6;59(9):1096-1109.e5.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MiniTurboID (BirA mutant)
    Use
    Golden gate / green gate compatible cloning vector
    Tags
    Linker and YFP
    Mutation
    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …
    Available since
    July 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    222434-plasmid-map-sequence-id-440831

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Showing: 1381 - 1400 of 5818 results