We narrowed to 6,752 results for: hwa

1. pCSJ125

   Plasmid

   #104344

   Purpose

   Express yeast MDH2-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.

   Depositor

   Alexander Varshavsky

   Article

   Chen et al Science. 2017 Jan 27;355(6323). pii: 355

   Insert

   MDH2
   

   Tags

   3xFLAG

   Expression

   Yeast

   Available Since

   July 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAW8ENdeI-cHAIAA7-spo5DSR

   Plasmid

   #49873

   Purpose

   for Cre-lox cassette exchange of C-terminal HA-auxin-inducible degron tagged gene sequences together with the S. pombe spo5 gene DSR element into the S. pombe urg1 locus

   Depositor

   Tony Carr

   Article

   Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

   Type

   Empty backbone

   Use

   Cre/Lox

   Tags

   IAA17 degron tag

   Available Since

   Jan. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDNA HA FAK Y576F

   Plasmid

   #50506

   Depositor

   Kenneth Yamada

   Article

   Tamura et al J Biol Chem. 1999 Jul 16;274(29):20693-7

   Insert

   protein tyrosine kinase, mutated
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Y576F

   Promoter

   CMV

   Available Since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

4. mRFP1-1

   Plasmid

   #63816

   Purpose

   A member of an RBS library for mRFP1 with different translation rates

   Depositor

   Howard Salis

   Article

   Farasat et al Mol Syst Biol. 2014 Jun 21;10:731. doi:

   Insert

   mRFP1
   

   Expression

   Bacterial

   Mutation

   Codon optimized for E.coli

   Promoter

   J23100

   Available Since

   May 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAWH-Rluc-let-7-Poly(A)

   Plasmid

   #50556

   Purpose

   Expression of luciferase reporter mRNA in S2 cell

   Depositor

   Yukihide Tomari

   Article

   Fukaya et al Mol Cell. 2012 Dec 28;48(6):825-36. doi:

   Insert

   Luciferase-let7-Poly(A)
   

   Use

   Luciferase

   Expression

   Insect

   Promoter

   Actin5C

   Available Since

   Jan. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFA6a-kanMX6-P41nmt1-mYFP

   Plasmid

   #105141

   Purpose

   Yeast gene targeting

   Depositor

   Thomas Pollard, Jian-Qiu Wu

   Article

   Wu et al Dev Cell. 2003 Nov;5(5):723-34.

   Insert

   kanMX6-P41nmt1-mYFP
   

   Tags

   41nmt1-mYFP

   Expression

   Bacterial and Yeast

   Mutation

   A206K

   Promoter

   41nmt1

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. Ptrc::RpaA D53A

   Plasmid

   #105229

   Purpose

   inducible expression of unphosphorylatable variant of RpaA (D53A)

   Depositor

   Erin O'Shea

   Article

   Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

   Insert

   rpaA driven by Ptrc promoter
   

   Use

   Cyanobacteria cloning vector

   Mutation

   D53 mutated to A

   Promoter

   ptrc

   Available Since

   Jan. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. mRFP1-9

   Plasmid

   #63818

   Purpose

   A member of an RBS library for mRFP1 with different translation rates

   Depositor

   Howard Salis

   Article

   Farasat et al Mol Syst Biol. 2014 Jun 21;10:731. doi:

   Insert

   mRFP1
   

   Expression

   Bacterial

   Mutation

   Codon optimized for E.coli

   Promoter

   J23100

   Available Since

   May 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUCBB-lacZa

   Plasmid

   #32563

   Depositor

   Claudia Schmidt-dannert

   Article

   Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

   Insert

   LacZ-alpha
   

   Use

   Synthetic Biology

   Tags

   His

   Expression

   Bacterial

   Promoter

   Consitutive lac

   Available Since

   Nov. 8, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pClneoEGFP human RASSF6b

    Plasmid

    #37021

    Depositor

    Yutaka Hata

    Article

    Ikeda et al Sci Signal. 2009 Sep 29;2(90):ra59.

    Insert

    RASSF6b (RASSF6 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

11. pDGE3

    Plasmid

    #79442

    Purpose

    recipient plasmid [multiplexing]; pPcUbi:Cas9, nptII

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

    Insert

    ccdB (ccdB(letD) )
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    BsaI site eliminated

    Available Since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDGE64

    Plasmid

    #79446

    Purpose

    recipient plasmid [single nuclease]; pPcUbi:Cas9, nptII

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

    Insert

    ccdB (ccdB(letD) )
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    BsaI site eliminated

    Available Since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDGE1

    Plasmid

    #79440

    Purpose

    recipient plasmid [multiplexing]; p35S:Cas9, nptII

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

    Insert

    ccdB (ccdB(letD) )
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    BsaI site eliminated

    Available Since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. Wnt5A Non-STOP

    Plasmid

    #35893

    Depositor

    Marian Waterman

    Article

    Najdi et al Differentiation. 2012 Sep;84(2):203-13.

    Insert

    Wnt5A (WNT5A Human)
    

    Use

    Entry vector

    Mutation

    None

    Available Since

    May 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. Wnt10B STOP

    Plasmid

    #35884

    Depositor

    Marian Waterman

    Article

    Najdi et al Differentiation. 2012 Sep;84(2):203-13.

    Insert

    Wnt10B (WNT10B Human)
    

    Use

    Entry vector

    Mutation

    None

    Available Since

    May 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. XE41 Rat frizzled-1-CS2+

    Plasmid

    #16821

    Depositor

    Randall Moon

    Article

    Yang-Snyder et al Curr Biol. 1996 Oct 1. 6(10):1302-6.

    Insert

    rat frizzled 1 (Fzd1 Rat)
    

    Use

    Xenopus expression

    Available Since

    April 7, 2008

    Availability

    Academic Institutions and Nonprofits only

17. pAVPR2_TCS(Q’L)_HA-dCas9(N)_P2A-Puro-WPRE

    Plasmid

    #101112

    Purpose

    Encodes dCas9(N) fused to AVPR2

    Depositor

    Tudor Fulga

    Article

    Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

    Insert

    AVPR2-dCas9(N)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag and HA

    Expression

    Mammalian

    Available Since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. AlphaE

    Plasmid

    #124527

    Purpose

    pColE1_AlphaE

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial, Insect, and Mamm…

    Available Since

    June 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. Wnt2 STOP

    Plasmid

    #35869

    Depositor

    Marian Waterman

    Article

    Najdi et al Differentiation. 2012 Sep;84(2):203-13.

    Insert

    Wnt2 (WNT2 Human)
    

    Use

    Entry vector

    Mutation

    Silent Mutation G (816) --> A

    Available Since

    May 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBDKRB2_TCS(Q’L)_HA-dCas9(N)_DHFR

    Plasmid

    #101109

    Purpose

    Encodes dCas9(N)-DHFR fused to BDKRB2

    Depositor

    Tudor Fulga

    Article

    Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

    Insert

    BDKRB2-dCas9(N)-DHFR
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag and HA

    Expression

    Mammalian

    Available Since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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