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pMST694_BB3_pTet-on_mttA_trpC_pcoxA:pyrGtrunc_AMA2.8

Plasmid

#90292

Purpose

BB3 for mttA integration with homologous flanks at pyrG locus, hygromycin resistance

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Insert

mttA

Use

A niger

Expression

Bacterial

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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SREC 1: abu-1::gfp

Plasmid

#21824

Depositor

David Ron

Article

Urano et al J Cell Biol. 2002 Aug 19. 158(4):639-46.

Insert

abu-1 promoter (abu-1 Nematode)

Tags

GFP

Expression

Worm

Available Since

Oct. 8, 2009

Availability

Academic Institutions and Nonprofits only

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pEGFP_mNTAQ1

Plasmid

#65486

Purpose

Mouse Ntaq1-EGFP reporter

Depositor

Alexander Varshavsky

Article

Wang et al Mol Cell. 2009 Jun 26;34(6):686-95. doi:

Insert

Ntaq1 (Wdyhv1 Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 21, 2015

Availability

Academic Institutions and Nonprofits only
pRK5-HA-Sestrin2-E451A

Plasmid

#73674

Purpose

overexpression

Depositor

David Sabatini

Article

Wolfson et al Science. 2016 Jan 1;351(6268):43-8. doi:

Insert

Sesn2 (SESN2 Human)

Tags

HA

Expression

Mammalian

Mutation

E451A changed Glutamate 451 to Alanine

Promoter

CMV

Available Since

March 30, 2016

Availability

Academic Institutions and Nonprofits only
pRK5-HA-Sestrin2-L261A

Plasmid

#73672

Purpose

overexpression

Depositor

David Sabatini

Article

Wolfson et al Science. 2016 Jan 1;351(6268):43-8. doi:

Insert

Sesn2 (SESN2 Human)

Tags

HA

Expression

Mammalian

Mutation

L261A changed Leucine 261 to Alanine

Promoter

CMV

Available Since

March 30, 2016

Availability

Academic Institutions and Nonprofits only
pBad_crtEBI_2

Plasmid

#63803

Purpose

expresses crtEBI genes for neurosporene production

Depositor

Howard Salis

Article

Farasat et al Mol Syst Biol. 2014 Jun 21;10:731. doi:

Insert

crtEBI operon

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

PBAD

Available Since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

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pTYB2-HCR1

Plasmid

#37233

Depositor

Jon Lorsch

Article

Mitchell et al Mol Cell. 2010 Sep 24;39(6):950-62.

Insert

HRC1 (HCR1 Budding Yeast)

Expression

Bacterial

Promoter

T7

Available Since

July 16, 2012

Availability

Academic Institutions and Nonprofits only
pBad_crtEBI_52

Plasmid

#63808

Purpose

expresses crtEBI genes for neurosporene production

Depositor

Howard Salis

Article

Farasat et al Mol Syst Biol. 2014 Jun 21;10:731. doi:

Insert

crtEBI operon

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

PBAD

Available Since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

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pBad_crtEBI_37

Plasmid

#63807

Purpose

expresses crtEBI genes for neurosporene production

Depositor

Howard Salis

Article

Farasat et al Mol Syst Biol. 2014 Jun 21;10:731. doi:

Insert

crtEBI operon

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

PBAD

Available Since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

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pUC19-RPL41A

Plasmid

#37223

Depositor

Jon Lorsch

Article

Mitchell et al Mol Cell. 2010 Sep 24;39(6):950-62.

Insert

RPL41A (RPL41A Budding Yeast)

Expression

Bacterial

Available Since

July 5, 2012

Availability

Academic Institutions and Nonprofits only
XE8 Xwnt-8 myc

Plasmid

#16868

Depositor

Randall Moon

Article

Christian et al Genes Dev. 1993 Jan. 7(1):13-28.

Insert

Xwnt-8 myc (wnt8a.L Frog)

Use

Xenopus expression

Tags

myc

Available Since

March 13, 2008

Availability

Academic Institutions and Nonprofits only
pWZL Hygro-NMYC TRF2 deltaM

Plasmid

#16354

Depositor

Titia de Lange

Article

Smogorzewska et al EMBO J. 2002 Aug 15. 21(16):4338-48.

Insert

hTRF2 (TERF2 Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

Deleted Myb Domain (aa454-500)

Available Since

May 14, 2008

Availability

Academic Institutions and Nonprofits only
XE225 mouse Chibby-pCS2+

Plasmid

#16823

Depositor

Randall Moon

Article

Takemaru et al Nature. 2003 Apr 24. 422(6934):905-9.

Insert

mouse Chibby-pCS2+ (cby Mouse)

Use

Xenopus expression

Available Since

March 13, 2008

Availability

Academic Institutions and Nonprofits only
DE_Thr6XHisFLAG

Plasmid

#139949

Purpose

Protein tags

Depositor

Raffaela Abbriano

Article

Jaramillo-Madrid et al Pharmaceuticals (Basel). 2020 Dec 21;13(

Insert

Thrombin6XHisFLAGStop

Use

Synthetic Biology

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pJH123

Plasmid

#172146

Purpose

Microscopy inclusion body assay

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

pCDF.B.(SD4)CD

Expression

Bacterial

Mutation

PylBCD operon (RBS inserted for PylC)

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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PAJ408

Plasmid

#104150

Purpose

dCas9 plasmid expressing full length mKate2 mRNA without RBS

Depositor

Alfonso Jaramillo

Article

Galizi et al CRISPR J. 2020 Oct;3(5):398-408. doi: 10

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

pLtetO-1

Available Since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pWDY1228

Plasmid

#138594

Purpose

Expresses NatL2-R156K in E.coli

Depositor

James Naismith

Article

Song et al Angew Chem Int Ed Engl. 2020 Jan 5. doi:

Insert

phenylacetate-coenzyme A ligase

Tags

6-His

Mutation

changed Arginine 156 to Lysine

Promoter

T7

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pHBSTC159

Plasmid

#124353

Purpose

Plasmid expressing inactive NpuDnaBΔ283 mini-intein inserted into CcdA antitoxin

Depositor

Hideo Iwai

Article

Beyer et al Chembiochem. 2019 Apr 8. doi: 10.1002/cb

Insert

NpuDnaBΔ283 (C1G) mini-intein

Tags

CcdA-C (SFAD)

Expression

Bacterial

Mutation

Deletion of 283-residue endonuclease domain, C1G

Promoter

STC

Available Since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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rpaA_NS1

Plasmid

#101829

Purpose

drives expression of rpaA from the NS1 site in the S. elongatus PCC7942 genome

Depositor

Erin O'Shea

Article

Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

Insert

RpaA with its native promoter

Use

Cyanobacteria cloning vector

Available Since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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P0050::rpaA

Plasmid

#101830

Purpose

drives expression of rpaA from an rpaA-independent promoter

Depositor

Erin O'Shea

Article

Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

Insert

RpaA with p0050 promoter (+ gentamicin resistance casette)

Use

Cloning vector

Available Since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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