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pCMV5 Flag-Smad2 EPSM

Plasmid

#14933

Depositor

Joan Massague

Article

Kretzschmar et al Genes Dev. 1999 Apr 1. 13(7):804-16.

Insert

Smad2 EPSM (SMAD2 Human)

Tags

Flag

Expression

Mammalian

Mutation

Erk/Pro-directed kinase site mutant (EPSM). T220V…

Available Since

May 17, 2007

Availability

Academic Institutions and Nonprofits only
LPCX Smad2 deltaSSMS

Plasmid

#12637

Depositor

Rik Derynck

Article

Choy et al J Cell Biol. 2000 May 1. 149(3):667-82.

Insert

Smad2 (SMAD2 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Last 4 aa's deleted.

Available Since

Oct. 10, 2006

Availability

Academic Institutions and Nonprofits only
pGLTR-FP-GFP

Plasmid

#58242

Purpose

GATEWAY-compatible lentiviral RNAi delivery vector; SFFV-driven eGFP marker

Depositor

Stephan Geley

Article

Sigl et al PLoS One. 2014 May 19;9(5):e97764. doi:

Insert

attR1-Cm(R)-CcdB-attR2

Use

Lentiviral and RNAi; Gateway destionation vector

Expression

Mammalian

Promoter

no

Available Since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pGEX Smad2 (L+MH2)

Plasmid

#15730

Depositor

Joan Massague

Article

He et al Cell. 2006 Jun 2. 125(5):929-41.

Insert

Smad2 (L+MH2) (SMAD2 Human)

Tags

GST

Expression

Bacterial

Mutation

amino acids 187-467 (linker and MH2 regions).

Available Since

Sept. 7, 2007

Availability

Academic Institutions and Nonprofits only
CS2 Flag-Smad2 (100-467)

Plasmid

#14928

Depositor

Joan Massague

Article

Hata et al Nature. 1997 Jul 3. 388(6637):82-7.

Insert

Smad2 (100-467) (SMAD2 Human)

Use

Xenopus/mammalian/avian/zebrafish

Tags

Flag

Mutation

amino acids 100-467

Available Since

May 17, 2007

Availability

Academic Institutions and Nonprofits only
CS2 Flag-Smad2 (1-185)

Plasmid

#14929

Depositor

Joan Massague

Article

Hata et al Nature. 1997 Jul 3. 388(6637):82-7.

Insert

Smad2 (1-185) (SMAD2 Human)

Use

Xenopus/mammalian/avian/zebrafish

Tags

Flag

Mutation

amino acids 1-185

Available Since

May 17, 2007

Availability

Academic Institutions and Nonprofits only
pCMV5 Flag-Smad2 (1-456)

Plasmid

#14958

Depositor

Joan Massague

Article

Hata et al Nature. 1997 Jul 3. 388(6637):82-7.

Insert

Smad2 (SMAD2 Human)

Tags

Flag

Expression

Mammalian

Mutation

Amino acids 1-456 (tail deletion)

Promoter

CMV

Available Since

May 17, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 3xFLAG TPP1 88-249

Plasmid

#53549

Purpose

mammalian expression of TPP1 OB-fold domain

Depositor

Kathleen Collins

Article

Sexton et al J Biol Chem. 2012 Oct 5;287(41):34455-64

Insert

TPP1 (ACD Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

aa 88-249

Promoter

CMV

Available Since

May 29, 2014

Availability

Academic Institutions and Nonprofits only
pMKR07

Plasmid

#240097

Purpose

A CRISPR‑Cas9 system for knock‑out and knock‑in of high molecular weight DNA enables module‑swapping of the pikromycin synthase in its native host

Depositor

Adrian Keatinge-Clay

Article

Wang et al Res Sq [Preprint]. 2025 Mar 27:rs.3.rs-6

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

ermE* promoter

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-3xFLAG-SMAD1-active

Plasmid

#222579

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of 3xFLAG-SMAD1

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

SMAD1 (SMAD1 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

S487E, S489E

Available Since

Aug. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-RIPK3[K5A]-FLAG

Plasmid

#199326

Purpose

mutated ubiquitination site (ubiquitination inhibited)

Depositor

Adrian Press

Article

Hoff et al Cell Death Dis. 2023 Apr 18;14(4):275. d

Insert

Receptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)

Tags

FLAG

Expression

Mammalian

Mutation

changed Lysine 5 to Alanine

Available Since

June 13, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-RIPK3[K5R]-FLAG

Plasmid

#199325

Purpose

mutated ubiquitination site (ubiquitination active)

Depositor

Adrian Press

Article

Hoff et al Cell Death Dis. 2023 Apr 18;14(4):275. d

Insert

Receptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)

Tags

FLAG

Expression

Mammalian

Mutation

changed Lysine 5 to Arginine

Available Since

June 13, 2023

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP-redesigned-human-Caspase-4_ANR261ENP_D315H_M318K_T352K

Plasmid

#183357

Purpose

Expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

Insert

Caspase-4 with ICE activity (CASP4 Human)

Use

Retroviral

Tags

Myc

Mutation

ANR261ENP, D315H, M318K, T352K

Promoter

MESV

Available Since

May 5, 2022

Availability

Academic Institutions and Nonprofits only

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p415TEF-NYFP

Plasmid

#99555

Purpose

Expresses Swi1 NQ-YFP to monitor Swi1 state or induce [SWI+].

Depositor

Liming Li

Article

Hines et al PLoS Genet. 2011 Feb;7(2):e1001309. doi:

Insert

Swi1 N-rich region (AA1-327) (SWI1 Budding Yeast)

Tags

YFP

Expression

Yeast

Promoter

TEF1

Available Since

Aug. 30, 2017

Availability

Academic Institutions and Nonprofits only

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p3HD1RLuc2

Plasmid

#236276

Purpose

Mammalian expression of human dopamine D1 receptor; N-terminal HA tag, C-terminal Rluc8

Depositor

Vsevolod Gurevich

Article

Gimenez et al J Biol Chem. 2012 Aug 24;287(35):29495-5

Insert

dopamine D1 receptor (DRD1 Human)

Tags

3xHA and Rluc8

Expression

Mammalian

Promoter

CMV

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pCGN N-Ras wt

Plasmid

#14723

Depositor

Adrienne Cox

Article

Fiordalisi et al Methods Enzymol. 2001;332:3-36. doi: 10.

Insert

N-ras (NRAS Human)

Tags

HA

Expression

Mammalian

Available Since

Aug. 29, 2007

Availability

Academic Institutions and Nonprofits only

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RSV-Flag-Brd4

Plasmid

#86616

Purpose

Flag tagged mouse Brd4 expression construct for cell transfection

Depositor

Mario García-Domínguez

Article

Garcia-Gutierrez et al J Cell Sci. 2012 Aug 1;125(Pt 15):3671-8

Insert

Brd4 (Brd4 Mouse)

Tags

Flag

Expression

Mammalian

Promoter

RSV

Available Since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

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RSV-Flag-Brd2

Plasmid

#86614

Purpose

Flag tagged mouse Brd2 expression construct for cell transfection

Depositor

Mario García-Domínguez

Article

Garcia-Gutierrez et al J Cell Sci. 2012 Aug 1;125(Pt 15):3671-8

Insert

Brd2 (Brd2 Mouse)

Tags

Flag

Expression

Mammalian

Promoter

RSV

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-RIPK3[S199D][S227D]-FLAG

Plasmid

#199324

Purpose

double mutated phosphorylation site (kinase acitivity and MLKL interaction active)

Depositor

Adrian Press

Article

Hoff et al Cell Death Dis. 2023 Apr 18;14(4):275. d

Insert

Receptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)

Tags

FLAG

Expression

Mammalian

Mutation

changed Serine 199 and 227 to Aspartic Acid

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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iRFP682-Smad2

Plasmid

#118943

Purpose

Mammalian expression of human Smad2 protein

Depositor

Won Do Heo

Article

Li et al ACS Synth Biol. 2018 Feb 16;7(2):443-451

Insert

iRFP682-Smad2 (SMAD2 Human)

Tags

iRFP682

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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