We narrowed to 2,869 results for: human cdnas

1. pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry WT

   Plasmid

   #163991

   Purpose

   Mammalian expression vector encoding codon-optimized human NLRP1 CARD with a C-terminal mCherry tag for imaging

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1 6A NLRP1 UPA-CARD-FLAG W1460A

   Plasmid

   #163989

   Purpose

   Mammalian expression vector encoding human NLRP1 UPA-CARD (dimer interface mutant) with a C-terminal FLAG tag

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   W1460A

   Promoter

   Available since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1 6A NLRP1 UPA-CARD-FLAG Y1445A

   Plasmid

   #163987

   Purpose

   Mammalian expression vector encoding human NLRP1 UPA-CARD (dimer interface mutant) with a C-terminal FLAG tag

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Y1445A

   Promoter

   Available since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1 6A NLRP1 UPA-CARD-FLAG R1427E

   Plasmid

   #163986

   Purpose

   Mammalian expression vector encoding human NLRP1 UPA-CARD (filament-deficient mutant) with a C-terminal FLAG tag

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   R1427E

   Promoter

   Available since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1 6A NLRP1 UPA-CARD-FLAG E1414R

   Plasmid

   #163985

   Purpose

   Mammalian expression vector encoding human NLRP1 UPA-CARD (filament-deficient mutant) with a C-terminal FLAG tag

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   E1414R

   Promoter

   Available since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1 6A NLRP1 UPA-CARD-FLAG E1411R

   Plasmid

   #163984

   Purpose

   Mammalian expression vector encoding human NLRP1 UPA-CARD (filament-deficient mutant) with a C-terminal FLAG tag

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   E1411R

   Promoter

   Available since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1 6A NLRP1 UPA-CARD-FLAG D1401R

   Plasmid

   #163983

   Purpose

   Mammalian expression vector encoding human NLRP1 UPA-CARD (filament-deficient mutant) with a C-terminal FLAG tag

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   R1401R

   Promoter

   Available since

   Feb. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1 6A NLRP1 UPA-CARD-FLAG E1397R

   Plasmid

   #163982

   Purpose

   Mammalian expression vector encoding human NLRP1 UPA-CARD (filament-deficient mutant) with a C-terminal FLAG tag

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   E1397R

   Promoter

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1 6A NLRP1 UPA-CARD-FLAG R1392E

   Plasmid

   #163981

   Purpose

   Mammalian expression vector encoding human NLRP1 UPA-CARD (filament-deficient mutant) with a C-terminal FLAG tag

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   R1392E

   Promoter

   Available since

   Jan. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1 6A NLRP1 UPA-CARD-FLAG R1386E

    Plasmid

    #163980

    Purpose

    Mammalian expression vector encoding human NLRP1 UPA-CARD (filament-deficient mutant) with a C-terminal FLAG tag

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    R1386E

    Promoter

    Available since

    Jan. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. (344) pcDNA3.1-HA-nGFP-(EAAAK)4-OGA(544-706)

    Plasmid

    #168097

    Purpose

    HA- tagged GFP nanobody fused to human OGA stalk domain residues (544-706). Used in combination with myc-OGA(1-400) to create split-OGA.

    Depositor

    Christina Woo

    Article

    Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

    Insert

    HA-nGFP-(EAAAK)4-OGA(544-706)
    

    Use

    Tags

    HA-Tag

    Expression

    Mammalian

    Mutation

    Promoter

    T7/CMV

    Available since

    May 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDNA-gp78-FLAG RING mutant C356G H361A

    Plasmid

    #61751

    Purpose

    express C-terminally FLAG tagged human gp78

    Depositor

    Yihong Ye

    Article

    Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

    Insert

    gp78 (AMFR Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    C356G H361A; P179L, F262L, Y315C, and D346G (ple…

    Promoter

    CMV

    Available since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

13. (367) pcDNA3.1-HA-nUbc-(EAAAK)4-OGA(544-706)

    Plasmid

    #168099

    Purpose

    HA- tagged Ubc6E nanobody fused to human OGA stalk domain residues (544-706). Used in combination with myc-OGA(1-400) to create split-OGA

    Depositor

    Christina Woo

    Article

    Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

    Insert

    HA-nUbc-OGA(544-706)
    

    Use

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    OGA truncated to contain residues 544-706

    Promoter

    T7/CMV

    Available since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1 Flag RdgBbeta (long form) splice variant 1

    Plasmid

    #58275

    Purpose

    mammalian expression of human RdgBbeta splice variant 1

    Depositor

    Shamshad Cockcroft

    Article

    Garner et al Biochem J. 2011 Oct 1;439(1):97-111. doi

    Insert

    RdgBbeta (PITPNC1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA3.2-Cx43-M100/125/147/213-siResist

    Plasmid

    #49858

    Purpose

    Encodes human connexin 43 with M100, M125, M147, and M213 mutated to L and wobble mutations conferring resistance against an siRNA

    Depositor

    Robin Shaw

    Article

    Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13

    Insert

    Connexin 43 (GJA1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Methionine100, Methionine 125, Methionine 147, an…

    Promoter

    CMV

    Available since

    Dec. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA5 FRT TO myc Separase delta 55/Non-Cleavable

    Plasmid

    #59827

    Purpose

    Allows the integration of myc Separase delta 55/Non-Cleavable in the genome and Tet-inducible expression.

    Depositor

    Stephen Taylor

    Article

    Holland et al J Biol Chem. 2007 Aug 24;282(34):24623-3

    Insert

    Separase (ESPL1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    delta 55/Non-Cleavable (E1483R, R1486E, E1503R, R…

    Promoter

    hybrid human cytomegalovirus (CMV)/TetO2 promoter

    Available since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-CD16.7-hPC1-eGFP-3HA

    Plasmid

    #108401

    Purpose

    Express cytoplasmic tail human PC1 from amino acid 4077 to 4302 with a membrane anchor

    Depositor

    Gregory Germino

    Article

    Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

    Insert

    PKD1 (PKD1 Human)
    

    Use

    Tags

    eGFP-3HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. (271) pcDNA3.1-HA-nEPEA-(EAAAK)3-OGA(544-706)

    Plasmid

    #168102

    Purpose

    HA- tagged EPEA nanobody fused to human OGA stalk domain residues (544-706). Used in combination with myc-OGA(1-400) to create split-OGA

    Depositor

    Christina Woo

    Article

    Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

    Insert

    HA-nEPEA-(EAAAK)3-OGA(544-706)
    

    Use

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    OGA truncated to contain residues 544-706

    Promoter

    T7/CMV

    Available since

    May 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. 221-pCAGGS-HA-AID-CTCFcDNA-AID-eGFP-2A-blasticidin

    Plasmid

    #195101

    Purpose

    Vector to introduce human CTCF cDNA tagged with eGFP and two AIDs in the cells. Blasticidin selection (2A-fusion). Auxin-inducible degron system.

    Depositor

    Job Dekker

    Article

    Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

    Insert

    CTCF (CTCF Human)
    

    Use

    Tags

    AID, HA, and eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    pCAGGS

    Available since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA4 h-Stx3 resistant to shRNA #304-2xMyc/His

    Plasmid

    #99746

    Purpose

    Expresses human Stx3 with a C-term myc-myc-his that is resistant to shRNA #304 from Sigma Aldrich.

    Depositor

    Thomas Weimbs

    Article

    Giovannone et al Mol Biol Cell. 2017 Oct 15;28(21):2843-2

    Insert

    Stx3 (STX3 Human)
    

    Use

    Tags

    myc-myc-his

    Expression

    Mammalian

    Mutation

    Made six (6) silent mutations to confer resistanc…

    Promoter

    CMV/TO

    Available since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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