We narrowed to 10,856 results for: kars;

1. Circular 200,100 with interspersed loops (SMAD4)

   Plasmid

   #180191

   Purpose

   AAV vector carrying a guide RNA targeting the human SMAD4 mRNA

   Depositor

   Prashant Mali

   Article

   Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

   Insert

   Circular 200,100 guide RNA with interspersed loops
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6

   Available since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLVX-opto-mRipK3

   Plasmid

   #208783

   Purpose

   Inducibly expresses mouse RipK3 fused to light activatable Cry2

   Depositor

   Petr Broz

   Article

   Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

   Insert

   mCherry mRipK3 Cry2 (Ripk3 Mouse)
   

   Use

   Lentiviral

   Tags

   Cry2 and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTSK561

   Plasmid

   #190816

   Purpose

   For C-terminal -Halo Tagging of a gene of interest with URA3 selection marker in yeast S. cerevisiae

   Depositor

   Gunjan Mehta, Tatiana Karpova

   Article

   Ball et al Nucleic Acids Res. 2016 Dec 1;44(21):e16

   Insert

   HaloTag
   

   Use

   Bacterial cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLVX-opto-mCasp8

   Plasmid

   #208782

   Purpose

   Inducibly expresses mouse caspase8 fused to light-activatable Cry2

   Depositor

   Petr Broz

   Article

   Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

   Insert

   mCherry mCaspase8 Cry2 (Casp8 Mouse)
   

   Use

   Lentiviral

   Tags

   Cry2 and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLX304 GDF11-V5 blast

   Plasmid

   #83098

   Purpose

   Lentiviral vector for constitutive expression of human GDF11 with C-terminal V5 tag

   Depositor

   Kevin Janes

   Article

   Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

   Insert

   GDF11 (GDF11 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJK1

   Plasmid

   #111722

   Purpose

   to express human BLM protein in yeast cells

   Depositor

   Ian Hickson

   Article

   Karow et al J Biol Chem. 1997 Dec 5;272(49):30611-4.

   Insert

   BLM (BLM Human)
   

   Use

   Tags

   hexahistidine

   Expression

   Yeast

   Mutation

   a C-terminal hexahistidine tag was added, a yeast…

   Promoter

   GAL1

   Available since

   Aug. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. PARP1-c009

   Plasmid

   #216872

   Purpose

   Bacterial expression vector for E. coli producing PARP1 catalytic protein (without Regulatory domain); with N-terminal CFP fusion

   Depositor

   Lari Lehtiö

   Article

   Dhakar et al Sci Rep. 2024 Feb 16;14(1):3875. doi: 10

   Insert

   PARP1 catalytic domain (no Regulatory Domain) (PARP1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. SiC-V2-Cas9G7

   Plasmid

   #133043

   Purpose

   Doxycycline-inducible SiC-V2 vector with Cas9G7 (sgRNA 7 targeting SpCas9 gene). In cells coexpressing Cas9 nuclease, this construct causes self-inactivation/editing of SpCas9 gene upon Dox addition.

   Depositor

   Sriram Neelamegham

   Article

   Kelkar et al Mol Ther. 2019 Sep 12. pii: S1525-0016(1

   Insert

   Tet repressor
   

   Use

   Lentiviral

   Tags

   Cerulean

   Expression

   Mutation

   Promoter

   Available since

   Nov. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair3

   Plasmid

   #98974

   Purpose

   Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 37bp 3' overhangs. TagBFP.

   Depositor

   Jan Karlseder

   Article

   Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

   Insert

   LMNA sgRNAs pair 3 and Cas9(D10A)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. PXL

    Plasmid

    #75349

    Purpose

    PX330 derived. Bbs1 & annealed oligos to insert guide strand. BstB1+Pac1 of PXL and FUX-/pRubiX- T2A-Cas9 for choice of sgRNA, viral backbone, and fluorescent protein. Pac1 to introduce 2nd sgRNA.

    Depositor

    Bryan Luikart

    Article

    Williams et al Sci Rep. 2016 May 10;6:25611. doi: 10.10

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDEINO1

    Plasmid

    #179472

    Purpose

    Multi-host shuttle plasmid for D. radiodurans, E. coli, S. cerevisiae, S. meliloti, and P. tricornutum; contains an oriT for transfer via conjugation

    Depositor

    Bogumil Karas

    Article

    Brumwell et al ACS Synth Biol. 2022 Mar 18;11(3):1068-1

    Inserts

    ori
    cat
    

    Use

    Synthetic Biology; Bacterial and yeast cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLX302 GDF11-V5 puro

    Plasmid

    #83097

    Purpose

    Lentiviral vector for constitutive expression of human GDF11 with C-terminal V5 tag

    Depositor

    Kevin Janes

    Article

    Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

    Insert

    GDF11 (GDF11 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

13. pcdna3 Flag JIP3b

    Plasmid

    #53458

    Purpose

    interacts with MAPK signaling module

    Depositor

    Roger Davis

    Article

    Kelkar et al Mol Cell Biol. 2000 Feb;20(3):1030-43.

    Insert

    mitogen-activated protein kinase 8 interacting protein 3 (Mapk8ip3 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pSC5GGv2

    Plasmid

    #188604

    Purpose

    pSC5 Golden-Gate Cloning Plasmid (BsaI - Site 2)

    Depositor

    Bogumil Karas

    Article

    Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

    Type

    Empty backbone

    Use

    Synthetic Biology; Diatom expression

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAGE2.0-i

    Plasmid

    #194639

    Purpose

    Empty pAGE2.0 plasmid with pBAD inducible promoter

    Depositor

    Bogumil Karas

    Article

    Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

    Type

    Empty backbone

    Use

    Synthetic Biology; Diatom expression

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair4

    Plasmid

    #98975

    Purpose

    Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 94bp 3' overhangs. TagBFP.

    Depositor

    Jan Karlseder

    Article

    Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

    Insert

    LMNA sgRNAs pair 4 and Cas9(D10A)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLEXm-GST-ATG14

    Plasmid

    #99329

    Purpose

    Component for mammalian expression of recombinant PI3KC3-C1 complex

    Depositor

    James Hurley

    Article

    Baskaran et al Elife. 2014 Dec 9;3. doi: 10.7554/eLife.

    Insert

    ATG14 (ATG14 Human)
    

    Use

    Tags

    GST-TEV tag

    Expression

    Mammalian

    Mutation

    Codon optimized

    Promoter

    CMV

    Available since

    Aug. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. All-in-one split-ADAR2(E488Q, N496F)

    Plasmid

    #170148

    Purpose

    AAV vector carrying ADAR2-DDC(E488Q, N496F)-λN-P2A-MCP-ADAR2-DDN with a nuclear export signal

    Depositor

    Prashant Mali

    Article

    Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

    Insert

    ADAR2-DDC(E488Q, N496F)-λN-P2A-MCP-ADAR2-DDN
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. LightR-iCre-miRFP670

    Plasmid

    #162158

    Purpose

    Expresses LightR-iCre-miRFP670 in mammalian cells

    Depositor

    Andrei Karginov

    Article

    Shaaya et al Elife. 2020 Sep 23;9. pii: 60647. doi: 1

    Insert

    LightR-iCre-miRFP670
    

    Use

    Tags

    NLS and miRFP670

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcdna3 T7 JIP3a

    Plasmid

    #53457

    Purpose

    interacts with MAPK signaling module

    Depositor

    Roger Davis

    Article

    Kelkar et al Mol Cell Biol. 2000 Feb;20(3):1030-43.

    Insert

    mitogen-activated protein kinase 8 interacting protein 3 (Mapk8ip3 Mouse)
    

    Use

    Tags

    T7

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 22, 2014

    Availability

    Academic Institutions and Nonprofits only