We narrowed to 4,157 results for: pCMV mammalian

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pCMV-PARP1-3xFlag-7xKQ

Plasmid

#111576

Purpose

Expresses full length PARP1 with K to Q mutations in automodification domain in mammalian cells

Depositor

Thomas Muir

Article

Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

Insert

PARP1 (PARP1 Human)

Tags

3xFlag

Expression

Mammalian

Mutation

lysine to glutamine mutation at the following ami…

Promoter

CMV

Available Since

July 18, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-SARS-CoV-2-Nsp3PLpro

Plasmid

#204208

Purpose

Mammalian expression of SARS-CoV-2 Nsp3

Depositor

Allan Weissman

Article

Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

Insert

SARS-CoV-2 Nsp3 (Wuhan-Hu-1; https://www.addgene.org/141257/) (ORF1ab )

Tags

FLAGx1

Expression

Mammalian

Mutation

PLpro of Nsp3

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-myc3-Mdm2 D367A

Plasmid

#52058

Purpose

expression of non-cleavable Mdm2 in mammalian cells

Depositor

Tyler Jacks, Trudy Oliver

Article

Oliver et al Mol Cell. 2011 Jul 8;43(1):57-71. doi: 1

Insert

Mdm2 D367A (MDM2 Human)

Tags

myc3

Expression

Mammalian

Mutation

D367A

Promoter

CMV

Available Since

March 13, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-SARS-CoV-2 Nsp3TMC111A

Plasmid

#204207

Purpose

Mammalian expression of SARS-CoV-2 Nsp3

Depositor

Allan Weissman

Article

Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

Insert

SARS-CoV-2 Nsp3 (Wuhan-Hu-1; https://www.addgene.org/141257/) (ORF1ab )

Tags

FLAGx1

Expression

Mammalian

Mutation

Cys to Ala in active site of PLpro

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-SARS-CoV-2 Nsp3TM

Plasmid

#204206

Purpose

Mammalian expression of SARS-CoV-2 Nsp3

Depositor

Allan Weissman

Article

Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

Insert

SARS-CoV-2 Nsp3 (Wuhan-Hu-1; https://www.addgene.org/141257/) (ORF1ab )

Tags

FLAGx1

Expression

Mammalian

Mutation

none, from obtained clone

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pcmv3-STING-C88S-FLAG

Plasmid

#198187

Purpose

Mammalian expression of FLAG-tagged mouse STING1 C88S

Depositor

Yun Zhao

Article

Li et al Cell Rep. 2023 Mar 28;42(3):112145. doi:

Insert

TMEM173 (Sting1 Mouse)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

April 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV 3xFLAG-ATG3 C264A

Plasmid

#129297

Purpose

Expresses 3xFLAG-ATG3 C264A in mammalian cells

Depositor

Robin Ketteler

Article

Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

Insert

Ubiquitin-like-conjugating enzyme ATG3 (ATG3 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Cysteine 264 to Alanine

Promoter

CMV

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-myc3-Mdm2 p60

Plasmid

#52059

Purpose

expression of truncated Mdm2 in mammalian cells

Depositor

Tyler Jacks, Trudy Oliver

Article

Oliver et al Mol Cell. 2011 Jul 8;43(1):57-71. doi: 1

Insert

Mdm2 p60 (truncated) (MDM2 Human)

Tags

myc3

Expression

Mammalian

Mutation

p60 (truncated at D367, DVDP motif)

Promoter

CMV

Available Since

March 20, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-hA3A-eBE-Y132D

Plasmid

#113429

Purpose

Expresses hA3A-eBE-Y132D in mammalian cells

Depositor

Jia Chen

Article

Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

Insert

hA3A-eBE-Y132D (APOBEC3A Human, S. pyogenes and Bacteriophage PBS2)

Use

CRISPR

Expression

Mammalian

Mutation

hAPOBEC3A_Y132D

Promoter

CMV

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-myc3-Mdm2 D367E

Plasmid

#52057

Purpose

expression of non-cleavable Mdm2 in mammalian cells

Depositor

Tyler Jacks, Trudy Oliver

Article

Oliver et al Mol Cell. 2011 Jul 8;43(1):57-71. doi: 1

Insert

Mdm2 D367E (MDM2 Human)

Tags

myc3

Expression

Mammalian

Mutation

D367E

Promoter

CMV

Available Since

March 13, 2014

Availability

Academic Institutions and Nonprofits only
pCMV 3xFLAG-ATG3 K243R

Plasmid

#129301

Purpose

Expresses pCMV 3xFLAG-ATG3 K243R in mammalian cells

Depositor

Robin Ketteler

Article

Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

Insert

Ubiquitin-like-conjugating enzyme ATG3 (ATG3 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Lysine 243 to Arginine

Promoter

CMV

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-ABEMAX-NLS-P2A_BLAST

Plasmid

#135355

Purpose

Expression of ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) with dual C-terminal NLS in a single operon encoding P2A blasticidine resistance

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

ABEMax-2XNLS

Use

CRISPR

Expression

Mammalian

Mutation

WT

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-Gpr45-Y287C-3xFlag

Plasmid

#245986

Purpose

Express 3xFlag C-terminal tagged mouse GPR45-Y287C mutant transiently via transfection in mammalian cells.

Depositor

Zhao Zhang

Article

Xun et al Science. 2025 Jun 5;388(6751):eadp3989.

Insert

Gpr45 (Gpr45 Mouse)

Tags

3xFlag

Expression

Mammalian

Mutation

Y287C

Promoter

CMV

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV5D HA-PPM1H S124A/S211A

Plasmid

#208370

Purpose

Mammalian expression of HA-tagged human PPM1H S124A/S211A

Depositor

Suzanne Pfeffer

Article

Wondwossen M Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

HA-PPM1H S124A/S211A (PPM1H Human)

Expression

Mammalian

Mutation

S124A/S211A

Available Since

Dec. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-HA_aa 1-542 POLK

Plasmid

#201585

Purpose

Expresses the N-terminal clasp domain and catalytic domain (aa 1-542) of human DNA polymerase kappa with a HA tag in mammalian cells

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

Polymerase kappa (POLK Human)

Tags

HA

Expression

Mammalian

Mutation

Expresses amino acids 1-542 of DNA polymerase kap…

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_RKK/AAA POLI

Plasmid

#131240

Purpose

Mammalian expression of N-terminally FLAG-tagged DNA polymerase iota containing mutations R524A, K526A and K530A

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

DNA polymerase iota (POLI Human)

Tags

FLAG

Expression

Mammalian

Mutation

Contains point mutations that convert arginine re…

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV 3xFLAG-ATG3 allKR

Plasmid

#129298

Purpose

Expresses pCMV 3xFLAG-ATG3 allKR (all lysines mutated to arginine) in mammalian cells

Depositor

Robin Ketteler

Article

Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

Insert

Ubiquitin-like-conjugating enzyme ATG3 (ATG3 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

changed all 22 Lysine residues to Arginine

Promoter

CMV

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV 3xFLAG-ATG3 K295R

Plasmid

#129302

Purpose

Expresses pCMV 3xFLAG-ATG3 K295R in mammalian cells

Depositor

Robin Ketteler

Article

Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

Insert

Ubiquitin-like-conjugating enzyme ATG3 (ATG3 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Lysine 295 to Arginine

Promoter

CMV

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV5B-Flag-Smurf1 wt

Plasmid

#11752

Depositor

Jeff Wrana

Article

Zhu et al Nature. 1999 Aug 12. 400(6745):687-93.

Insert

SMURF1 (SMURF1 Human)

Tags

Flag

Expression

Mammalian

Available Since

May 12, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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pCMV-Caspase1-flag

Plasmid

#21142

Depositor

Junying Yuan

Article

Li et al J Cell Biol. 2008 Apr 21. 181(2):321-33.

Insert

caspase-1 (Casp1 Mouse)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

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