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pME_Cp_0_5a_009_mVenus

Plasmid

#235891

Purpose

mVenus C-tag

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

mVenus C-tag

Use

Synthetic Biology

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_3b_030_mCerulean

Plasmid

#235883

Purpose

mCerulean N-tag

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

mCerulean N-tag

Use

Synthetic Biology

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pMDC32B-B/c

Plasmid

#227963

Purpose

Expression plasmid with a ccdB cassette flanked with two inverted BsaI restriction sites. Allows the high throughput cloning of cloning inserts flanked with compatible 4-nt overhangs.

Depositor

Alberto Carbonell

Article

Cisneros et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

Insert

B/c

Expression

Plant

Promoter

35S

Available Since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

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pM-mT-Sapphire

Plasmid

#228616

Purpose

Mammalian expression of mT-Sapphire and extra insert, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

Insert

mT-Sapphire

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pLei-sfGFP-V2*D134*Y152*

Plasmid

#191112

Purpose

An E. coli sfGFP reporter with two TAG at positions 2 & 134 & 152

Depositor

Han Xiao

Article

Wu et al Protein Sci. 2022 Oct;31(10):e4443. doi:

Insert

superfolder green fluorescence protein

Tags

His tag

Expression

Bacterial

Mutation

changing V2 & D134 & Y152 to TAG

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pTBL3481 MTK3-mCherry

Plasmid

#226672

Purpose

MTK3 part containing the coding sequence of mCherry.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2025 Apr;21(4):512-521. d

Insert

mCherry

Use

Synthetic Biology

Expression

Mammalian

Mutation

Removed BbsI sites

Promoter

none

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pTBL3445 MTK3-mTagBFP2

Plasmid

#226662

Purpose

MTK3 part containing the coding sequence of mTagBFP2

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2025 Apr;21(4):512-521. d

Insert

mTagBFP2

Use

Synthetic Biology

Promoter

none

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pACYCDuet-Myc-MBP-AtIAA6-HA

Plasmid

#222710

Purpose

Expression MBP-AtIAA6-HA in in bacterial cells

Depositor

Lei Wu

Article

Pu et al Biomolecules. 2024 Sep 25;14(10):1209. d

Insert

AtIAA6 (IAA6 Mustard Weed)

Tags

HA and MBP

Expression

Bacterial

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pBWB507

Plasmid

#209325

Purpose

p15A-tetR-tet-ME-RBS-mRFP-ME-U1-Barcode-U2-dbI terminator-oriT-Cm. Plasmid backbone for fragment expression.

Depositor

Adam Arkin

Article

Biggs et al Mol Syst Biol. 2024 Oct 7. doi: 10.1038/

Insert

mRFP

Expression

Bacterial

Promoter

tet

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pTE5418_pMBP-GlcR

Plasmid

#221192

Purpose

Expression vector for 10x-MBP-GlcR (MGlcR). GlcR is a glycolate-responsive transcriptional repressor and the gene product of pden4400 from Paracoccus denitrificans, codon optimized for E. coli.

Depositor

Tobias Erb

Article

Barthel et al Synth Biol (Oxf). 2024 Sep 20;9(1):ysae0

Insert

glcR (pden4400 from Paracoccus denitrificans)

Tags

10x His tag with E. coli maltose binding protein …

Expression

Bacterial

Promoter

T7 promoter and T7 promoter-lacO

Available Since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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