We narrowed to 31,350 results for: Eng

1. pBiT3.1-N_SKIC8:1-305

   Plasmid

   #211038

   Purpose

   Mammalian expression of full-length human SKIC8. N-terminal HiBiT tag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al bioRxiv 2024.03.03.583197

   Insert

   SKIC8:M1-I305 (SKIC8 Human)
   

   Use

   Tags

   MVSGWRLFKKISGSSGGSSG

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   CMV

   Available since

   Feb. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBiT3.1-N_RBBP4:1-425

   Plasmid

   #211037

   Purpose

   Mammalian expression of full-length human RBBP4. N-terminal HiBiT tag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al bioRxiv 2024.03.03.583197

   Insert

   RBBP4:M1-S425 (RBBP4 Human)
   

   Use

   Tags

   MVSGWRLFKKISGSSGGSSG

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   CMV

   Available since

   Feb. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pRK-Myc-Smurf1

   Plasmid

   #13676

   Depositor

   Ying Zhang

   Article

   Yamashita et al Cell. 2005 Apr 8. 121(1):101-13.

   Insert

   Smurf1 (SMURF1 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 8, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBTK1017 (pNR)

   Plasmid

   #183131

   Purpose

   Control plasmid that does not express dsRNA

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al Science. 2020 Jan 31;367(6477):573-576.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. MMLV-RT

   Plasmid

   #181801

   Purpose

   MMLV-RT (3XFLAG-SV40-MMLVRT)

   Depositor

   Erik Sontheimer

   Article

   Liu et al Nat Biotechnol. 2022 Apr 4. pii: 10.1038

   Insert

   MMLV-RT
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. GsI-IIC-RT

   Plasmid

   #181806

   Purpose

   GsI-IIC RT

   Depositor

   Erik Sontheimer

   Article

   Liu et al Nat Biotechnol. 2022 Apr 4. pii: 10.1038

   Insert

   GsI-IIC-RT
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. BBa_J72113-BBa_J72103

   Plasmid

   #40785

   Depositor

   Christopher Anderson

   Article

   Kittleson et al ACS Synth Biol. 2012 Dec 21;1(12):583-9.

   Insert

   AraC-pBad + coi + cin + repL + pacA
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 31, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. PE-maturase-RT

   Plasmid

   #181807

   Purpose

   PE-E.r. maturase RT

   Depositor

   Erik Sontheimer

   Article

   Liu et al Nat Biotechnol. 2022 Apr 4. pii: 10.1038

   Insert

   PE-maturase-RT
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. BBa_J72110-BBa_J72094

   Plasmid

   #40778

   Depositor

   Christopher Anderson

   Article

   Kittleson et al ACS Synth Biol. 2012 Dec 21;1(12):583-9.

   Insert

   AraC-pBad + coi
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. BBa_J72110-BBa_J72097

    Plasmid

    #40779

    Depositor

    Christopher Anderson

    Article

    Kittleson et al ACS Synth Biol. 2012 Dec 21;1(12):583-9.

    Insert

    RhaRS-pRhaB + Coi
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCT-L7.5.1

    Plasmid

    #42900

    Depositor

    Dane Wittrup

    Article

    Hackel et al J Mol Biol. 2008 Sep 19;381(5):1238-52.

    Insert

    L7.5.1
    

    Use

    Tags

    HA and c-myc

    Expression

    Yeast

    Mutation

    Promoter

    Gal1-10

    Available since

    June 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pILGFP1F5

    Plasmid

    #165064

    Purpose

    Characterisation of TEF1 promoter using yEGFP as reporter gene and PGK1 terminator

    Depositor

    Claudia Vickers

    Article

    Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

    Insert

    P-URA3>KlURA3>T-AgTEF1-P-TEF1> (BamHI)yEGFP>T-PGK1-T-URA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    WT

    Promoter

    Available since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTPTP alpha (Y789F)

    Plasmid

    #17693

    Depositor

    Xinmin Zheng

    Article

    Zheng et al EMBO J. 2000 Mar 1. 19(5):964-78.

    Insert

    PTPalpha Y789F (PTPRA Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Y789F

    Promoter

    Available since

    April 7, 2008

    Availability

    Academic Institutions and Nonprofits only

14. pTPTP alpha (CCSS)

    Plasmid

    #17692

    Depositor

    Xinmin Zheng

    Article

    Zheng et al EMBO J. 2000 Mar 1. 19(5):964-78.

    Insert

    PTPalpha CCSS (PTPRA Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    C433S / C723S

    Promoter

    Available since

    April 24, 2008

    Availability

    Academic Institutions and Nonprofits only

15. maturase-RT

    Plasmid

    #181805

    Purpose

    E.r. maturase RT

    Depositor

    Erik Sontheimer

    Article

    Liu et al Nat Biotechnol. 2022 Apr 4. pii: 10.1038

    Insert

    maturase-RT
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRF032d

    Plasmid

    #156353

    Purpose

    Expresses heterologous rRNA

    Depositor

    Ahmed Badran

    Article

    Kolber et al Nat Commun. 2021 Jan 26;12(1):599. doi:

    Insert

    rRNA operon, orthogonal-antiRBS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    WT antiRBS engineered to orthogonal antiRBS, PS e…

    Promoter

    Available since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28a-AviTag-CC-S2

    Plasmid

    #233243

    Purpose

    Bacterial expression of Avi-CC-S2

    Depositor

    Khalid Salaita

    Article

    Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

    Insert

    AviTag-CC-S2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. DENV-3 strain Phillippines/H87/1956 NS2B-NS3 protease fusion with non cleavable linker

    Plasmid

    #228651

    Purpose

    Bacterial expression of the fusion of the NS2B-NS3 protease from Dengue virus serotype 3 for biochemical assays

    Depositor

    Frank von Delft, ASAP Discovery

    Insert

    Dengue 3 portion of NS2B protease fused to NS3
    

    Use

    Tags

    His-Twin Strep-Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 18, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. DENV-1 strain US/Hawaii/1944 NS2B-NS3 protease fusion with non cleavable linker

    Plasmid

    #228648

    Purpose

    Bacterial expression of the fusion of the NS2B-NS3 protease from Dengue virus serotype 1 for biochemical assay

    Depositor

    Frank von Delft, ASAP Discovery

    Insert

    Dengue 1 portion of NS2B protease fused to NS3
    

    Use

    Tags

    His-Twin Strep-Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 18, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pVP293

    Plasmid

    #222062

    Purpose

    GreenGate CD bridge module containing filler sequence, backbone domesticated of Esp3I sites.

    Depositor

    Wayne Parrott

    Article

    Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

    Insert

    Filler
    

    Use

    Synthetic Biology; Greengate compatible cloning v…

    Tags

    Expression

    Mutation

    Backbone Esp3i sites have been domesticated

    Promoter

    Available since

    Dec. 16, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

21. pVP294

    Plasmid

    #222063

    Purpose

    GreenGate DE bridge module containing filler sequence, backbone domesticated of Esp3I sites.

    Depositor

    Wayne Parrott

    Article

    Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

    Insert

    Filler
    

    Use

    Synthetic Biology; Greengate compatible cloning v…

    Tags

    Expression

    Mutation

    Backbone Esp3i sites have been domesticated

    Promoter

    Available since

    Dec. 16, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

22. pVP076

    Plasmid

    #222028

    Purpose

    Empty spectinomycin resistant GreenGate destination vector based on pGGP-AG. Domesticated of Esp3I sites in the backbone and chloramphenicol resistance gene.

    Depositor

    Wayne Parrott

    Article

    Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

    Type

    Empty backbone

    Use

    Synthetic Biology; Greengate compatible cloning v…

    Tags

    Expression

    Mutation

    All Esp3i sites domesticated

    Promoter

    Available since

    Dec. 16, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

23. pVP077

    Plasmid

    #222029

    Purpose

    Empty Kanamycin resistant GreenGate destination vector based on pGGP-AG. Domesticated of Esp3I sites in the backbone and chloramphenicol resistance gene.

    Depositor

    Wayne Parrott

    Article

    Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

    Type

    Empty backbone

    Use

    Synthetic Biology; Greengate compatible cloning v…

    Tags

    Expression

    Mutation

    All Esp3i sites domesticated

    Promoter

    Available since

    Dec. 16, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

24. Vector 1174K

    Plasmid

    #221017

    Purpose

    Fluorescent based sex separator in Anopheles species mosquitoes (SEPARATOR)

    Depositor

    Omar Akbari

    Insert

    Engineered dobulesex splicing module (Anopheles gambiae)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pHAGE2-TetO-miniSOX

    Plasmid

    #206366

    Purpose

    Expresses miniSOX in mammalian cells, for lentivirus generation.

    Depositor

    Ralf Jauch

    Article

    Hu et al Nucleic Acids Res. 2023 Aug 23:gkad597.

    Insert

    miniSOX
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET-GFP-miniSOX

    Plasmid

    #206381

    Purpose

    Expresses EGFP fused miniSOX in bacteria, for protein production.

    Depositor

    Ralf Jauch

    Article

    Hu et al Nucleic Acids Res. 2023 Aug 23:gkad597.

    Insert

    miniSOX
    

    Use

    Tags

    EGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pILGFP1D5

    Plasmid

    #165063

    Purpose

    Characterisation of yeast promoter using yEGFP as reporter gene and PGK1 terminator

    Depositor

    Claudia Vickers

    Article

    Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAC038

    Plasmid

    #156345

    Purpose

    Expresses heterologous rRNA

    Depositor

    Ahmed Badran

    Article

    Kolber et al Nat Commun. 2021 Jan 26;12(1):599. doi:

    Insert

    rRNA operon, orthogonal-antiRBS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    WT antiRBS engineered to orthogonal antiRBS, PS e…

    Promoter

    Available since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pILGFP1H5

    Plasmid

    #165065

    Purpose

    Characterisation of TEF1 promoter using yEGFP as reporter gene and PGK1 terminator.

    Depositor

    Claudia Vickers

    Article

    Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

    Insert

    P-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>T-PGK1-T-URA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    WT

    Promoter

    Available since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pILGFPY2A

    Plasmid

    #165066

    Purpose

    Characterisation of 2A-mediated bicistronic expression of Y-FAST and yEGFP

    Depositor

    Claudia Vickers

    Article

    Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

    Insert

    P-URA3>KlURA3>T-AgTEF1-P-TEF1>Y-FAST-ErB1.2A-yEGFP>T-PGK1-T-URA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    WT

    Promoter

    Available since

    March 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge