We narrowed to 2,693 results for: CCS

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pPB-cT3G-cERH-SOHLH1

Plasmid

#222922

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of SOHLH1

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

SOHLH1 (SOHLH1 Human)

Expression

Mammalian

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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Hygro-P2A-HA-FKBP-WAPL

Plasmid

#217666

Purpose

Repair template to endogenously tag WAPL with Hygro-P2A-HA-FKBP

Depositor

Daniel Gerlich

Article

Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

Insert

WapI (WAPL Human)

Tags

dTag (FKBP36V protein)

Expression

Bacterial and Mammalian

Promoter

Lac

Available Since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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pNeonGreen-C1-Nir1-631-894

Plasmid

#220089

Purpose

Truncated Nir1-LNS2 domain lacking N-terminal amphipathic helix; loses ability to bind PA

Depositor

Gerry Hammond

Article

Weckerly et al J Cell Biol. 2025 Nov 3;224(11):e2024051

Insert

PITPNM3 (PITPNM3 Human)

Tags

NeonGreen-GGSGG

Expression

Mammalian

Mutation

amino acids 631-894

Promoter

CMV

Available Since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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pNeonGreen-N1-Nir1-613-630

Plasmid

#220088

Purpose

N-terminal amphipathic helix of Nir1-LNS2 domain; loses ability to bind PA

Depositor

Gerry Hammond

Article

Weckerly et al J Cell Biol. 2025 Nov 3;224(11):e2024051

Insert

PITPNM3 (PITPNM3 Human)

Tags

GGSGG-NeonGreen

Expression

Mammalian

Mutation

amino acids 613-630

Promoter

CMV

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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WT_IFL_G1A-T2A_R

Plasmid

#200183

Purpose

WT PA14 I-F Leader and G1A-T2A Repeat

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Wildtype I-F Leader and a mutant I-F Locus (G1A-T2A)

Expression

Bacterial

Mutation

G1A-T2A in the I-F repeat

Available Since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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WT_IFL_G1A-T2A-A28C_R

Plasmid

#200185

Purpose

WT PA14 I-F Leader and G1A-T2A-A28C Repeat

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Wildtype I-F Leader and a mutant I-F Locus (G1A-T2A-A28C)

Expression

Bacterial

Mutation

G1A-T2A-A28C in the I-F repeat

Available Since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pRAW-457

Plasmid

#200213

Purpose

Strep-TEV-PaCas1 + PaCas2/3 (Cas1 H25A)

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

PaCas1, PaCas2-3

Tags

Strep II-TEV on Cas1

Expression

Bacterial

Mutation

Cas1 H25A mutation

Available Since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pRAW-458

Plasmid

#200214

Purpose

Strep-TEV-PaCas1 + PaCas2/3 (Cas1 E184A)

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

PaCas1, PaCas2-3

Tags

Strep II-TEV on Cas1

Expression

Bacterial

Mutation

Cas1 E184A mutation

Available Since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pRAW-459

Plasmid

#200215

Purpose

Strep-TEV-PaCas1 + PaCas2/3 (Cas1 K214A)

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

PaCas1, PaCas2-3

Tags

Strep II-TEV on Cas1

Expression

Bacterial

Mutation

Cas1 K214A mutation

Available Since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pRAW-460

Plasmid

#200216

Purpose

Strep-TEV-PaCas1 + PaCas2/3 (Cas2 R55E, N56D)

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

PaCas1, PaCas2-3

Tags

Strep II-TEV on Cas1

Expression

Bacterial

Mutation

Cas2 R55E-N56D mutations

Available Since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pRAW-461

Plasmid

#200217

Purpose

Strep-TEV-PaCas1 + PaCas2/3 (Cas2 K11D, R12E)

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

PaCas1, PaCas2-3

Tags

Strep II-TEV on Cas1

Expression

Bacterial

Mutation

Cas2 K11D-R12E mutations

Available Since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pRAW-462

Plasmid

#200218

Purpose

Strep-TEV-PaCas1 + PaCas2/3 (Cas2 K11D, R12E, R55E, N56D)

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

PaCas1, PaCas2-3

Tags

Strep II-TEV on Cas1

Expression

Bacterial

Mutation

Cas2 K11D-R12E, R55E-N56D mutations

Available Since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-EphB4

Plasmid

#200983

Purpose

Mammalian expression plasmid for myc-tagged EphB4

Depositor

Erik Procko

Article

Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

Insert

EphB4 (EPHB4 Human)

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pET21a(+)_co_L-HicDH

Plasmid

#185453

Purpose

CDS of L-HicDH codon optimized for expression in Synechocystis sp. PCC 6803 in pET21a(+).

Depositor

Wolfgang Kroutil

Article

Jurkas et al Eng Microbiol. 2021 Nov 26;2(1):100008.

Insert

L-HicDH from Lactobacillus confusus DSM 20196

Expression

Bacterial

Promoter

T7

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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pSEVA251_ Ptrc.x.tetO2::D-HicDH

Plasmid

#185456

Purpose

CDS of D-HicDH codon optimized for expression in Synechocystis sp. PCC 6803 under the control of the synthetic promoter Ptrc.x.tetO2 and the RBS BBa_B0030 in pSEVA251 plasmid.

Depositor

Wolfgang Kroutil

Article

Jurkas et al Eng Microbiol. 2021 Nov 26;2(1):100008.

Insert

D-HicDH from Lactobacillus paracasei DSM 20008

Expression

Bacterial

Promoter

Ptrc.x.tetO2

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-sgPLXNB2

Plasmid

#86151

Purpose

Cas9/CRISPR plasmid for cut in human PLXNB2 gene exon 3

Depositor

Roland Friedel

Article

Huang et al Commun Biol. 2021 Jan 29;4(1):145. doi:

Insert

Plexin-B2 (PLXNB2 Human)

Use

CRISPR

Available Since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only
pTBL405 CHYRON1 integration construct

Plasmid

#126442

Purpose

To integrate the CHYRON1 locus at HEK293site3.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Inserts

pU6-CHYRON1 hgRNA
pCMV-puro

Expression

Mammalian

Mutation

The SpCas9 sgRNA constant region is mutated to ma…

Promoter

CMV and human U6

Available Since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

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pLX304 PCSK5 (R511C)-V5 blast

Plasmid

#83104

Purpose

Lentiviral vector for constitutive expression of human mutant PC5A (R511C) with C-terminal V5 tag

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

PCSK5 (PCSK5 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Changed Arginine 511 to Cysteine

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
pLX304 PCSK5 (T288P)-V5 blast

Plasmid

#83101

Purpose

Lentiviral vector for constitutive expression of human mutant PC5A (T288P) with C-terminal V5 tag

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

PCSK5 (PCSK5 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Changed Threonine 288 to Proline

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
pLX304 PCSK5 (A270T)-V5 blast

Plasmid

#83102

Purpose

Lentiviral vector for constitutive expression of human mutant PC5A (A270T) with C-terminal V5 tag

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

PCSK5 (PCSK5 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Changed Alanine 270 to Threonine

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only