We narrowed to 2,053 results for: Mbp

1. zCD2

   Plasmid

   #122031

   Purpose

   Expresses histone deacetylase 6 (HDAC6) catalytic domain 2 (CD2), also known as zCD2, in Escherichia coli cells.

   Depositor

   David Christianson

   Article

   Hai et al Nat Chem Biol. 2016 Sep;12(9):741-7. doi

   Insert

   Zebrafish HDAC6 Catalytic Domain 2
   

   Use

   Tags

   His6-MBP tag with cleavable TEV site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCR1056

   Plasmid

   #71824

   Purpose

   Express Streptococcus pyogenes dCas9 (D10A, H840A) carrying two C-terminal SV40 NLS

   Depositor

   Jacob Corn

   Article

   Richardson et al Nat Biotechnol. 2016 Jan 20. doi: 10.103

   Insert

   T7pr_6xHis-MBP-TEV-SPydCas9 (D10A,H840A)-2xNLS
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCR1054

   Plasmid

   #71822

   Purpose

   Express Streptococcus pyogenes nCas9 (D10A) nickase carrying two C-terminal SV40 NLS

   Depositor

   Jacob Corn

   Article

   Richardson et al Nat Biotechnol. 2016 Jan 20. doi: 10.103

   Insert

   T7pr_6xHis-MBP-TEV-SPynCas9 (D10A)-2xNLS
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCR1003

   Plasmid

   #71821

   Purpose

   Express Streptococcus pyogenes dCas9 (D10A, H840A)

   Depositor

   Jacob Corn

   Article

   Richardson et al Nat Biotechnol. 2016 Jan 20. doi: 10.103

   Insert

   T7pr_10xHis-MBP-TEV-SPydCas9 (D10A, H840A)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCR1055

   Plasmid

   #71823

   Purpose

   Express Streptococcus pyogenes nCas9 (H840A) nickase carrying two C-terminal SV40 NLS

   Depositor

   Jacob Corn

   Article

   Richardson et al Nat Biotechnol. 2016 Jan 20. doi: 10.103

   Insert

   T7pr_6xHis-MBP-TEV-SPynCas9 (H840A)-2xNLS
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSHS293

   Plasmid

   #87348

   Purpose

   D435C/E945C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, lobe closure FRET construct

   Depositor

   Jennifer Doudna

   Article

   Sternberg et al Nature. 2015 Nov 5;527(7576):110-3. doi:

   Insert

   Cas9
   

   Use

   Tags

   His-MBP

   Expression

   Bacterial

   Mutation

   C80S/D435C/C574S/E945C

   Promoter

   T7

   Available since

   March 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTMS1

   Plasmid

   #87799

   Purpose

   For constructing MBP-SUMO-fusion proteins with N-terminal His tag and C-terminal strep II tag using "blunt-end" cloning.

   Depositor

   Yu-Zhu Zhang

   Article

   Zhang et al Mol Biotechnol. 2017 May;59(4-5):159-167

   Type

   Empty backbone

   Use

   Tags

   6xHis and Strep II

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSHS248

   Plasmid

   #87354

   Purpose

   S867C/N1054C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, HNH-2 FRET construct

   Depositor

   Jennifer Doudna

   Article

   Sternberg et al Nature. 2015 Nov 5;527(7576):110-3. doi:

   Insert

   Cas9
   

   Use

   Tags

   His-MBP

   Expression

   Bacterial

   Mutation

   C80S/C574S/S867C/N1054C

   Promoter

   T7

   Available since

   April 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTMS2

   Plasmid

   #87800

   Purpose

   For constructing MBP-SUMO-fusion proteins with N-terminal StrepII tag and C-terminal His tag using "blunt-end" cloning.

   Depositor

   Yu-Zhu Zhang

   Article

   Zhang et al Mol Biotechnol. 2017 May;59(4-5):159-167

   Type

   Empty backbone

   Use

   Tags

   6xHis and Strep II

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. TbSIR2RP1

    Plasmid

    #67242

    Purpose

    Determine its activity and specificity

    Depositor

    Cynthia Wolberger

    Article

    Fahie et al FEBS J. 2009 Dec;276(23):7159-76. doi: 1

    Insert

    TbSIR2RP1
    

    Use

    Tags

    MBP, TEV, Thrombin, HIS, and none

    Expression

    Bacterial

    Mutation

    Promoter

    p-lac

    Available since

    May 16, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pCri-1a-Strep

    Plasmid

    #61337

    Purpose

    Protein expression.

    Depositor

    F Xavier Gomis-Ruth

    Article

    Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

    Insert

    Green fluorescent protein
    

    Use

    Tags

    His-tag and MBP-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pDZ2087

    Plasmid

    #92414

    Purpose

    Expresses His-tagged mutant (L56V/S135G/S219V) TEV protease in E. coli

    Depositor

    David Waugh

    Article

    Raran-Kurussi et al Methods Mol Biol. 2017;1586:221-230. doi

    Insert

    TEV protease
    

    Use

    Tags

    MBP (self cleaving)

    Expression

    Bacterial

    Mutation

    L56V/S135G/S219V

    Promoter

    tac

    Available since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSHS212

    Plasmid

    #87346

    Purpose

    Cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid

    Depositor

    Jennifer Doudna

    Article

    Sternberg et al Nature. 2015 Nov 5;527(7576):110-3. doi:

    Insert

    Cas9
    

    Use

    Tags

    His-MBP

    Expression

    Bacterial

    Mutation

    C80S/C574S

    Promoter

    T7

    Available since

    March 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCri-1b

    Plasmid

    #61327

    Purpose

    Protein expression.

    Depositor

    F Xavier Gomis-Ruth

    Article

    Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

    Insert

    Green fluorescent protein
    

    Use

    Tags

    His-tag and MBP-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pMAL-BRT17

    Plasmid

    #45358

    Depositor

    Scott Banta

    Article

    Shur et al Biotechniques. 2013 Apr;54(4):197-206. d

    Insert

    17 Repeat Beta Roll Tag
    

    Use

    Tags

    Maltose Binding Protein (MBP)

    Expression

    Bacterial

    Mutation

    Promoter

    Ptac

    Available since

    June 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKEW397_AcrIIA15

    Plasmid

    #138285

    Purpose

    Expression of AcrIIA15 protein

    Depositor

    Jennifer Doudna

    Article

    Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

    Insert

    AcrIIA15
    

    Use

    CRISPR

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJAT25

    Plasmid

    #204310

    Purpose

    For generating targeting inversions. C-terminal split GFP, expressed in abdomen and mouthparts.

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Insert

    ie1-GFPc
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    ie1

    Available since

    Aug. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMAL-c2x-MT1

    Plasmid

    #32101

    Depositor

    Cristina Risco Ortiz, David DeRosier, Chris Mercogliano

    Article

    Mercogliano et al J Struct Biol. 2007 Oct;160(1):70-82. Ep

    Insert

    Metallothionein (Mt1 Mouse)
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    P-lac

    Available since

    Jan. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pJAT23

    Plasmid

    #204312

    Purpose

    For generating targeting inversions. C-terminal split GFP, expressed in eyes.

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Insert

    3XP3-GFPc
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    3XP3

    Available since

    Aug. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBA2257

    Plasmid

    #38151

    Depositor

    David Waugh

    Article

    Austin et al Protein Expr Purif. 2012 Mar;82(1):116-2

    Insert

    MBP-proMeCPA fusion protein
    

    Use

    Tags

    maltose binding protein

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Aug. 21, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge