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We narrowed to 10,667 results for: plasmids 101

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Showing: 1441 - 1460 of 10667 results

  5. pX330-U6-Chimeric_BB-CBh-hSpCas9-hGem(1/110)

    Plasmid
    #71707
    Purpose
    A human codon-optimized SpCas9 fused to first 110 amino acids of human GEMININ and chimeric guide RNA expression plasmid
    Depositor
    Giulio Draetta
    Article
    Gutschner et al Cell Rep. 2016 Feb 3. pii: S2211-1247(16
    Insert
    humanized S. pyogenes Cas9 fused to human Geminin (GMNN Human)
    Use
    CRISPR
    Tags
    3xFLAG and hGEM(1/110)
    Expression
    Mammalian
    Promoter
    CBh
    Available Since
    Feb. 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    71707-plasmid-map-sequence-id-424083

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  8. P530_SIX6-p2A-h2b-EGFP

    Plasmid
    #239101
    Purpose
    Human donor plasmid for integration of p2A-h2b-EGFP into the 3' end of SIX6. This includes the donor casette and a U6 driven guide sequence to be used with Cas9.
    Depositor
    Karl Wahlin
    Article
    Wahlin et al Front Cell Dev Biol. 2021 Nov 16;9:76472
    Insert
    SIX6 (SIX6 Human)
    Use
    Donor plasmid
    Tags
    p2A-h2b-EGFP
    Expression
    Mammalian
    Promoter
    Endogenous SIX6 promoter
    Available Since
    June 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    239101-plasmid-map-sequence-id-474411

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  10. pCMV-T7-ABE8e-nSpCas9-NG-P2A-EGFP (KAC999)

    Plasmid
    #185914
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized ABE8e-nSpCas9-NG-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9-NG=D10A/L1111R/D1135V/G1218R/E1219F/A1322…
    Promoter
    CMV and T7
    Available Since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185914-plasmid-map-sequence-id-367363

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  11. pCMV-T7-ABE8.20m-nSpCas9-NG-P2A-EGFP (KAC1164)

    Plasmid
    #185919
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized ABE8.20m-nSpCas9-NG-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9-NG=D10A/L1111R/D1135V/G1218R/E1219F/A1322…
    Promoter
    CMV and T7
    Available Since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185919-plasmid-map-sequence-id-367356

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  12. pCMV-T7-ABE8e-nSpCas9-VRQR-P2A-EGFP (KAC1309)

    Plasmid
    #185913
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9-VRQR(D10A/D1135V/G1218R/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized ABE8e-nSpCas9-VRQR-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9-VRQR=D10A/D1135V/G1218R/R1335Q/T1337R
    Promoter
    CMV and T7
    Available Since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185913-plasmid-map-sequence-id-367365

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  14. pCMV-T7-ABE8.20m-nSpCas9-VRQR-P2A-EGFP (KAC1166)

    Plasmid
    #185918
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9-VRQR(D10A/D1135V/G1218R/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized ABE8.20m-nSpCas9-VRQR-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9-VRQR=D10A/D1135V/G1218R/R1335Q/T1337R
    Promoter
    CMV and T7
    Available Since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185918-plasmid-map-sequence-id-367359

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  17. pFB1.HMBP-Prs.AEBP2iso2.mt1K2K

    Plasmid
    #247013
    Purpose
    Expresses human AEBP2iso2.mt1K2K (with 27 asp/glu-to-lys mutations) in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.
    Depositor
    Chen Davidovich
    Insert
    AEBP2iso2.mt1K2K (AEBP2 Human)
    Tags
    6xHis-MBP
    Expression
    Insect
    Mutation
    E37K, E38K, E39K, E40K, E41K, E42K, E43K, E44K, E…
    Promoter
    Polyhedrin
    Available Since
    Nov. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    247013-plasmid-map-sequence-id-493367

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  18. pFB1.HMBP-Prs.AEBP2iso2.mt1A2A

    Plasmid
    #247012
    Purpose
    Expresses human AEBP2iso2.mt1A2A (with 27 asp/glu-to-ala mutations) in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.
    Depositor
    Chen Davidovich
    Insert
    AEBP2iso2.mt1A2A (AEBP2 Human)
    Tags
    6xHis-MBP
    Expression
    Insect
    Mutation
    E37A, E38A, E39A, E40A, E41A, E42A, E43A, E44A, E…
    Promoter
    Polyhedrin
    Available Since
    Nov. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    247012-plasmid-map-sequence-id-493365

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  19. pCAMTA2-donor

    Plasmid
    #132501
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    CAMTA2 (CAMTA2 Human)
    Use
    CRISPR
    Tags
    GFP
    Mutation
    none
    Available Since
    March 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    132501-plasmid-map-sequence-id-274607

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  20. pCAMTA2.1.0-gDNA

    Plasmid
    #132456
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    CAMTA2 (CAMTA2 Human)
    Use
    CRISPR
    Available Since
    July 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    132456-plasmid-map-sequence-id-266911

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Showing: 1441 - 1460 of 10667 results