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We narrowed to 12,515 results for: plasmids 101

Showing: 1441 - 1460 of 12515 results

  1. Cas9/pTREX-n

    Plasmid
    #68708
    Purpose
    Expresses the fusion gene CAS9-HA-2xNLS-GFP in the pTREX-n backbone. This vector is used for cloning a specific sgRNA by BamHI, to be co-expressed with Cas9 for genome editing in Trypanosoma cruzi.
    Depositor
    Roberto Docampo
    Article
    Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.
    Insert
    Fusion gene Cas9-HA-2xNLS-GFP from vector pMJ920 (Addgene) .
    Use
    CRISPR
    Tags
    Fusion gene Cas9-HA-2xNLS-GFP from vector pMJ920 …
    Expression
    Mutation
    C4239T mutation that eliminates a BamHI restricti…
    Promoter
    Available Since
    Sept. 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    68708-plasmid-map-sequence-id-123989

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  9. p189

    Plasmid
    #65207
    Purpose
    Mutant EGFP expression plasmid for the creation of a heteroduplex DNA repair assay plasmid with pGEM5Z(+)-EGFP (p111). *Please see notes in comments section.
    Depositor
    LuZhe Sun
    Article
    Zhou et al Anal Biochem. 2009 May 1;388(1):167-9. d
    Insert
    EGFP
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Premature stop codon added to pGEM5Z(+)-EGFP (TGG…
    Promoter
    CMV
    Available Since
    June 29, 2015
    Availability
    Academic Institutions and Nonprofits only

  13. pPTG-GG-sg2+sg3

    Plasmid
    #127199
    Purpose
    Plasmid expressing sgRNA2 (TACCACATTTGTAGAGGTT) & sgRNA3 (CAATGTATCTTATCATGTC) as polycistronic tRNA-gRNA from single U6 promoter
    Depositor
    Jordan Green
    Article
    Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6
    Insert
    tRNA-gRNA
    Use
    Empty sgrna plasmid
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available Since
    May 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    127199-plasmid-map-sequence-id-248025

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  14. ADH1pro_GCN4_SIR4

    Plasmid
    #138620
    Purpose
    Transcription factor tagged with Sir4 used for Calling Cards experiments for binding measurement
    Depositor
    Rob Mitra
    Insert
    GCN4 (GCN4 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    ADH1
    Available Since
    June 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    138620-plasmid-map-sequence-id-334951

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  15. pAc5.1C-Fluc-CG33111_A

    Plasmid
    #145866
    Purpose
    Insect Expression of CG33111-3UTR
    Depositor
    Elisa Izaurralde
    Insert
    CG33111-3UTR (CG33111 Fly)
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available Since
    Feb. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    145866-plasmid-map-sequence-id-288311

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  16. pTS2308-Tier1-pGL3-PPGK-NLuc_TB

    Plasmid
    #169551
    Purpose
    Tier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' insulator sequence derived from the pGL3 plasmid (pGL3-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    pGL3-insulator-modulated PPGK-driven NanoLuc Luciferase - mTagBFP expression cassette
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PPGK
    Available Since
    June 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    169551-plasmid-map-sequence-id-364981

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  18. AAV:ITR-U6-sgRNA(backbone)-pCBh-Cre-WPRE-hGHpA-ITR

    Plasmid
    #60229
    Purpose
    Expresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.
    Depositor
    Feng Zhang
    Article
    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01
    Inserts
    sgRNA
    Cre recombinase
    Use
    AAV, CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    Cre-HA
    Expression
    Mammalian
    Mutation
    Promoter
    CBh and U6
    Available Since
    Oct. 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    60229-plasmid-map-sequence-id-419029

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Showing: 1441 - 1460 of 12515 results