We narrowed to 3,175 results for: tef

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pCas3

Plasmid

#229538

Purpose

pMV_hyg encoding Cas3(wt) and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning.

Depositor

Kevin Verstrepen

Publication

Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:

Insert

Cas3

Use

CRISPR

Expression

Bacterial and Yeast

Mutation

codon optimized for S.cerevisiae

Available since

Jan. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pRSII41N-TDH3pr-mNeon

Plasmid

#194536

Purpose

Low copy vector backbone with nourseothricin selectivity, encodes for TDH3(pr) driven expression of mNeon

Depositor

Patrick Gibney

Publication

Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

Insert

natAC

Expression

Yeast

Promoter

TDH3

Available since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pRSII42H-TDH3pr-mNeon

Plasmid

#194539

Purpose

High copy vector backbone with hygromycin B selectivity, encodes for TDH3(pr) driven expression of mNeon

Depositor

Patrick Gibney

Publication

Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

Insert

hphMX

Expression

Yeast

Promoter

TDH3

Available since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pBV58

Plasmid

#204603

Purpose

ERG11-3X Minidegron-5X FLAG-HIS3MX6

Depositor

Scott Moye-Rowley

Insert

Candida glabrata ERG11 (ERG11 )

Tags

auxin-inducible degron

Expression

Bacterial

Available since

July 11, 2024

Availability

Academic Institutions and Nonprofits only

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UG75-CDS1-3HA

Plasmid

#184760

Purpose

CDS1 overexpression. Diverts glycerolipid synthesis pathway towards phospholipids. Uses antibiotic resistance marker hphMX6.

Depositor

Zhiping Xie

Publication

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

CDS1 (CDS1 Budding Yeast)

Tags

3HA

Expression

Yeast

Promoter

CDS1

Available since

Feb. 17, 2023

Availability

Academic Institutions and Nonprofits only

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UG75-DGK1-3HA

Plasmid

#184759

Purpose

DGK1 overexpression. Promotes the formation of phosphatidate from diacylglycerol. Uses antibiotic resistance marker hphMX6.

Depositor

Zhiping Xie

Publication

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

DGK1 (DGK1 Budding Yeast)

Tags

3HA

Expression

Yeast

Promoter

DGK1

Available since

Feb. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pESC-NAT-Ago-Dcr

Plasmid

#160381

Purpose

Overexpression of Ago-Dcr fusion protein in yeast

Depositor

John McCusker

Insert

Ago- Dcr

Expression

Yeast

Promoter

GAL

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pZCAG

Plasmid

#129114

Purpose

Z. rouxii centromeric vector containing G418 resistance

Depositor

Paolo Giudici

Publication

Bizzarri et al Yeast. 2019 Dec;36(12):711-722. doi: 10.

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pICE LYS2-KanMx

Plasmid

#122899

Purpose

LYS2-KanMx ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Publication

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pICE LYS2-NatMx

Plasmid

#122900

Purpose

LYS2-NatMx ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Publication

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pICE TRP1-HphMx

Plasmid

#122901

Purpose

TRP1-HphMx ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Publication

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pFA6a-SRS:hphMX4

Plasmid

#134441

Purpose

PCR-based C-terminal SRS tag

Depositor

Robert Tomko

Publication

Nemec et al Yeast. 2020 May 13. doi: 10.1002/yea.347

Insert

SRS

Use

Pcr-based tagging in yeast

Available since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pFA6a-SRS:natMX4

Plasmid

#134444

Purpose

PCR-based C-terminal SRS tag

Depositor

Robert Tomko

Publication

Nemec et al Yeast. 2020 May 13. doi: 10.1002/yea.347

Insert

SRS

Use

Pcr-based tagging in yeast

Available since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pAV0816

Plasmid

#133510

Purpose

Expression of mTagBFP2 tagged CRIB domain to monitor active Cdc42

Depositor

Sophie Martin

Publication

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pAde6(PmeI)-p(act1)-CRIB(gic2aa2-181)-mTagBFP2-terminator(ScADH1)-bsdMX

Expression

Yeast

Available since

Jan. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pILGFPE9

Plasmid

#83545

Purpose

Used to evaluate the expression output of TEF1 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Publication

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

TEF1 promoter-yEGFP

Expression

Yeast

Promoter

TEF1

Available since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCRISPEY-GAL-Hyg

Plasmid

#232098

Purpose

Yeast CEN plasmid for galactose-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with HygMX selection

Depositor

Jeffrey Lewis

Publication

Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pFa6-TID-FKBP-3HA-HIS3MX6

Plasmid

#228025

Purpose

To integrate TID-FKBP fusion at yeast promoter; yeast BioID with HIS3MX6 marker

Depositor

Joseph Reese

Publication

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID-FKBP

Tags

HA tag

Expression

Yeast

Available since

Nov. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pFA6a-BASU-HA3-KanMX6

Plasmid

#227978

Purpose

To insert biotin ligase, BASU, at the C-terminus of genes; yeast BioID, KanMX6 marker

Depositor

Joseph Reese

Publication

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

BASU

Tags

HA tag

Expression

Yeast

Available since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pRSII41H-TDH3pr-mNeon

Plasmid

#194538

Purpose

Low copy vector backbone with hygromycin B selectivity, encodes for TDH3(pr) driven expression of mNeon

Depositor

Patrick Gibney

Publication

Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

Insert

hphMX

Expression

Yeast

Promoter

TDH3

Available since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pPath-pR-integrase

Plasmid

#200000

Purpose

Entry vector for biosynthetic pathway. pR-phiC31 integrase

Depositor

Farren Isaacs

Publication

Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

Insert

Pathways

Expression

Bacterial

Available since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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