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PLKO.1-USP10-3'UTR

Plasmid

#136056

Purpose

USP10 shRNA (Targeting 3'UTR) inserted into the PLKO.1 plasmid (GCCTCTCTTTAGTGGCTCTTT)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

USP10 (USP10 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

April 24, 2020

Availability

Academic Institutions and Nonprofits only

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pCAS9-BFP empty

Plasmid

#80976

Purpose

Cas9, TagBFP, and sgRNA expression

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Type

Empty backbone

Expression

Mammalian

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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PLKO.1-USP10-CDS

Plasmid

#136057

Purpose

USP10 shRNA (Targeting CDS) inserted into the PLKO.1 plasmid (CCTATGTGGAAACTAAGTATT)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

USP10 (USP10 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

April 24, 2020

Availability

Academic Institutions and Nonprofits only

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EMM65

Plasmid

#49042

Purpose

Backbone contains SHD/C 0.5 Domain TALE N-terminal: TAL C-terminus: LSD1: Isoform 2

Depositor

Bradley Bernstein, Eric Mendenhall

Article

Mendenhall et al Nat Biotechnol. 2013 Sep 8. doi: 10.1038

Type

Empty backbone

Use

Tale lsd1 expression vector

Tags

LSD1

Expression

Mammalian

Promoter

EF1 alpha

Available Since

Oct. 31, 2013

Availability

Academic Institutions and Nonprofits only

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EMM67

Plasmid

#49043

Purpose

Backbone contains SHD/G 0.5 Domain TALE N-terminal TALE C-terminus, LSD1: Isoform 2

Depositor

Bradley Bernstein, Eric Mendenhall

Article

Mendenhall et al Nat Biotechnol. 2013 Sep 8. doi: 10.1038

Type

Empty backbone

Use

Tale lsd1 expression vector

Tags

LSD1

Expression

Mammalian

Promoter

EF1 alpha

Available Since

Oct. 31, 2013

Availability

Academic Institutions and Nonprofits only

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CEL-IFP2-SOScat

Plasmid

#208746

Purpose

catalytic domain of SOS2 fused with near ir fluorescent protein and CEL tag

Depositor

Xiaokun Shu

Article

Chung et al ACS Cent Sci. 2023 Jul 7;9(7):1466-1479.

Insert

CEL-IFP2-SOScat

Expression

Mammalian

Available Since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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psi-CHECK3-3xBoxB-MS2

Plasmid

#136011

Purpose

3 repeats of BoxB and MS2 hairpins inserted into the 3'UTR of Renilla luciferase in the psiCHECK3 plasmid for the Tethering assay

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

No gene (Artificial)

Use

Luciferase

Expression

Mammalian

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pRVdG-4mCherry

Plasmid

#52488

Purpose

Expresses mCherry

Depositor

Sebastian Seung

Article

Weible et al J Neurosci. 2010 Dec 8;30(49):16509-13.

Insert

mCherry

Use

Rabies virus

Promoter

CMV

Available Since

Aug. 22, 2014

Availability

Academic Institutions and Nonprofits only

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ZIF-NLS-EGFP(Y66F)-HOTag6

Plasmid

#208749

Purpose

part B of nls-SparkDrop with non-fluorescent eGFP

Depositor

Xiaokun Shu

Article

Chung et al ACS Cent Sci. 2023 Jul 7;9(7):1466-1479.

Insert

ZIF-NLS-EGFP(Y66F)-HOTag6

Expression

Mammalian

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pMEH275 NLS dTomato

Plasmid

#168210

Purpose

Mammalian expression of nuclear-localized dTomato

Depositor

Joshua Leonard

Article

Hung et al J Extracell Vesicles. 2016 May 13;5:3102

Insert

NLS dTomato

Use

Lentiviral

Tags

NLS

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-VAPA(1-242) K87D/M89D

Plasmid

#18875

Depositor

Axel Brunger

Article

Kaiser et al Structure. 2005 Jul . 13(7):1035-45.

Insert

VAP-A (Vapa Rat)

Tags

GFP

Expression

Mammalian

Mutation

double point mutant K87D / M89D

Available Since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only
pBD-0016

Plasmid

#12345

Depositor

Axel Brunger

Article

DeLaBarre et al Mol Cell. 2006 May 19. 22(4):451-62.

Insert

VCP R313K (Vcp Mouse)

Tags

His6

Expression

Bacterial

Mutation

R313K, I206V*(see below)

Available Since

Aug. 24, 2006

Availability

Academic Institutions and Nonprofits only
pOZ-C 16 E7

Plasmid

#13705

Depositor

Karl Munger

Article

Huh et al Proc Natl Acad Sci U S A. 2005 Aug 9. 10

Insert

HPV 16 E7 (E7 Human)

Tags

FLAG and HA

Expression

Mammalian

Available Since

March 12, 2007

Availability

Academic Institutions and Nonprofits only
pCIP-GFP

Plasmid

#79012

Purpose

For mammalian expression of GFP.

Depositor

Reuben Shaw

Article

Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

Insert

eGFP

Expression

Mammalian

Promoter

pCAGGs

Available Since

July 5, 2016

Availability

Academic Institutions and Nonprofits only
pOZ-N 16 E7

Plasmid

#13706

Depositor

Karl Munger

Article

Huh et al Proc Natl Acad Sci U S A. 2005 Aug 9. 10

Insert

HPV 16 E7 (E7 Human)

Tags

FLAG and HA

Expression

Mammalian

Available Since

March 12, 2007

Availability

Academic Institutions and Nonprofits only
pMiR-HPV16-E7

Plasmid

#53696

Purpose

Luciferase reporter assay for microRNA binding on HPV16 E7

Depositor

Edward Chan

Article

Jung et al Mol Cancer. 2014 Apr 8;13(1):80. doi: 10

Insert

HPV16-E7

Use

Luciferase

Available Since

June 17, 2014

Availability

Academic Institutions and Nonprofits only
pNCMV 18E6no*

Plasmid

#37456

Purpose

Expression of HPV 18E6no* in mammalian cells

Depositor

Karl Munger

Article

Spangle et al J Virol. 2012 May 2.

Insert

HPV 18E6no*

Tags

Flag and HA

Expression

Mammalian

Mutation

V44A

Promoter

CMV

Available Since

Aug. 13, 2012

Availability

Academic Institutions and Nonprofits only
pGKO2

Plasmid

#63617

Purpose

a binary Agrobacterium vector carrying a modified HSVtk gene as a negative selection marker against ectopic transformants.

Depositor

Seogchan Kang

Article

Khang et al Fungal Genet Biol. 2005 Jun;42(6):483-92

Type

Empty backbone

Use

Fungal expression

Available Since

July 27, 2015

Availability

Academic Institutions and Nonprofits only

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pCY 3080-01

Plasmid

#36226

Depositor

Anne Robinson

Article

Young et al Yeast. 2012 Mar;29(3-4):119-36. doi: 10.

Insert

yEmCitrine

Use

Yeast cassette for pcr and homologous recombinati…

Tags

yEpolylinker-yEmCitrine-kanMX

Available Since

Aug. 3, 2012

Availability

Academic Institutions and Nonprofits only

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pRVdG-N-P-M-EGFP-SynPhRFP-L

Plasmid

#52483

Purpose

Expresses EGFP and Synaptophysin-TagRFP-T

Depositor

Sebastian Seung

Article

Wickersham et al Nat Commun. 2013;4:2332. doi: 10.1038/nc

Inserts

EGFP
Synaptophysin-TagRFP-T

Use

Rabies virus

Tags

TagRFP-T

Expression

Mammalian

Promoter

CMV

Available Since

May 23, 2014

Availability

Academic Institutions and Nonprofits only

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