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pISH-Pou6f1

Plasmid

#74356

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Pou6f1. Not for gene expression.

Depositor

Peter Mombaerts

Publication

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

POU domain, class 6, transcription factor 1 (Pou6f1 Mouse)

Available since

April 12, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pISH-Pou2f1

Plasmid

#74355

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Pou2f1. Not for gene expression.

Depositor

Peter Mombaerts

Publication

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

POU domain, class 2, transcription factor 1 (Pou2f1 Mouse)

Available since

April 12, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pISH-Pbx3

Plasmid

#74352

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Pbx3. Not for gene expression.

Depositor

Peter Mombaerts

Publication

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

pre B cell leukemia homeobox 3 (Pbx3 Mouse)

Available since

April 12, 2016

Availability

Academic Institutions and Nonprofits only

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pISH-Dlx4

Plasmid

#74343

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Dlx4. Not for gene expression.

Depositor

Peter Mombaerts

Publication

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

distal-less homeobox 4 (Dlx4 Mouse)

Available since

April 8, 2016

Availability

Academic Institutions and Nonprofits only

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pBabe 3XFLAG-S/T3A-GATA6-3XAU1 puro

Plasmid

#72611

Purpose

For mammalian expression of an N-terminally triple FLAG-tagged and C-terminally triple AU1-tagged full length human GATA6 with triple alanine mutations on aminoacids#33,#34 and #37

Depositor

Kevin Janes

Publication

Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

Insert

GATA binding protein 6 (GATA6 Human)

Use

Retroviral

Tags

3XAU1 and 3XFLAG

Expression

Mammalian

Mutation

changed serine33, threonine34 and serine37 to ala…

Available since

Feb. 18, 2016

Availability

Academic Institutions and Nonprofits only
pEMS1245

Plasmid

#29126

Purpose

Expression of the eGFP reporter gene was not detected in the brain using eGFP. Untested in the eye.

Depositor

Elizabeth Simpson

Publication

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple20 (CARTPT Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Available since

Nov. 4, 2011

Availability

Academic Institutions and Nonprofits only

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pEMS1243

Plasmid

#29125

Purpose

Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested

Depositor

Elizabeth Simpson

Publication

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple18 (CARTPT Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Available since

Nov. 4, 2011

Availability

Academic Institutions and Nonprofits only

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pEMS1492

Plasmid

#29240

Purpose

Expression of the reporter gene was not detected in the brain and eye using LacZ.

Depositor

Elizabeth Simpson

Publication

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple21 (CARTPT Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

Intron-LacZ-NLS

Available since

Oct. 20, 2011

Availability

Industry, Academic Institutions, and Nonprofits

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pIS53 SERP1 3'UTR

Plasmid

#14499

Depositor

David Bartel

Publication

Lim et al Nature. 2005 Feb 17. 433(7027):769-73.

Insert

SERP1 3'UTR (SERP1 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Available since

March 7, 2007

Availability

Academic Institutions and Nonprofits only
pIS53 SERP1 3'UTR mut

Plasmid

#14500

Depositor

David Bartel

Publication

Lim et al Nature. 2005 Feb 17. 433(7027):769-73.

Insert

SERP1 3'UTR mutant (SERP1 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

CATTCC’s were changed to AAGTAC's

Available since

March 7, 2007

Availability

Academic Institutions and Nonprofits only
pSB700 Cerulean-Weissman scaffold

Plasmid

#79377

Purpose

Cas9 gRNA variant to recruit ms2 proteins

Depositor

George Church

Publication

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

scaffold

Use

CRISPR

Expression

Mammalian

Availability

Academic Institutions and Nonprofits only

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pSB700 Cerulean-Zhang2.0

Plasmid

#79378

Purpose

Cas9 gRNA variant to recruit ms2 proteins

Depositor

George Church

Publication

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

zhang 2.0

Use

CRISPR

Expression

Mammalian

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSB700 lenti gRNA Cerulean-zhang2.0-Weiss-chimera1

Plasmid

#79379

Purpose

Cas9 gRNA variant to recruit ms2 proteins

Depositor

George Church

Publication

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

Chimera 1

Use

CRISPR and Lentiviral

Expression

Mammalian

Availability

Academic Institutions and Nonprofits only

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pSB700 lenti gRNA Cerulean-zhang2.0-Weiss-chimera4

Plasmid

#79382

Purpose

Cas9 gRNA variant to recruit ms2 proteins

Depositor

George Church

Publication

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

Chimera 4

Use

CRISPR and Lentiviral

Expression

Mammalian

Availability

Academic Institutions and Nonprofits only

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pWUKI Cas1 Cas2 E9Q

Plasmid

#80091

Purpose

Encodes Cas1 and Cas2 E9Q

Depositor

George Church

Publication

Shipman et al Science. 2016 Jun 9. pii: aaf1175.

Inserts

Cas1 (cas1 )
Cas2 (cas2 )
CRISPR Array (bp1542-2899)

Expression

Bacterial

Mutation

E9Q

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pWUKI Cas1 V134L Cas2

Plasmid

#80092

Purpose

Encodes Cas1 V134L and Cas2

Depositor

George Church

Publication

Shipman et al Science. 2016 Jun 9. pii: aaf1175.

Inserts

Cas1 (cas1 )
Cas2 (cas2 )
CRISPR Array

Expression

Bacterial

Mutation

V134L

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pWUKI Cas1 V33A Cas2

Plasmid

#80093

Purpose

Encodes Cas1 V33A and Cas2

Depositor

George Church

Publication

Shipman et al Science. 2016 Jun 9. pii: aaf1175.

Inserts

Cas1 (cas1 )
Cas2 (cas2 )
CRISPR Array

Expression

Bacterial

Mutation

V33A

Availability

Academic Institutions and Nonprofits only

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pWUKI Cas1 V190I Cas2

Plasmid

#80094

Purpose

Encodes Cas1 V190I and Cas2

Depositor

George Church

Publication

Shipman et al Science. 2016 Jun 9. pii: aaf1175.

Inserts

Cas1 (cas1 )
Cas2 (cas2 )
CRISPR Array

Expression

Bacterial

Mutation

V190I

Availability

Academic Institutions and Nonprofits only

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pWUKI Cas1 P202Q Cas2

Plasmid

#80096

Purpose

Encodes Cas1 P202Q and Cas2

Depositor

George Church

Publication

Shipman et al Science. 2016 Jun 9. pii: aaf1175.

Inserts

Cas1 (cas1 )
Cas2 (cas2 )
CRISPR Array

Expression

Bacterial

Mutation

P202Q

Availability

Academic Institutions and Nonprofits only

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pWUKI Cas1 P212L Cas2

Plasmid

#80097

Purpose

Encodes Cas1 P212L and Cas2

Depositor

George Church

Publication

Shipman et al Science. 2016 Jun 9. pii: aaf1175.

Inserts

Cas1 (cas1 )
Cas2 (cas2 )
CRISPR Array

Expression

Bacterial

Mutation

P212L

Availability

Academic Institutions and Nonprofits only

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