We narrowed to 1,704 results for: sfGFP

1. LLP789_αGCN4-DNMT3A-CO

   Plasmid

   #211783

   Purpose

   SunTag counterpart binding domain, aGCN4, fused to DNA methyltransferase DNMT3A, with GFP selection

   Depositor

   Ryan Lister

   Article

   Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

   Insert

   aGCN4-DNMT3A
   

   Tags

   3xTy1

   Expression

   Mammalian

   Mutation

   Last 300 bp codon optimised (CO) to detect DNMT3A…

   Promoter

   pEF1a and pSV40

   Available Since

   Dec. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOPS0383

   Plasmid

   #133233

   Purpose

   Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.

   Depositor

   Philip Poole

   Article

   Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117

   Insert

   constructed with NoP (pOGG113), sfGFP (pOGG037) and T-pharma (pOGG003)
   

   Expression

   Bacterial

   Mutation

   Domesticated for Golden Gate cloning

   Available Since

   April 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSR353

   Plasmid

   #125078

   Purpose

   Expression of narL under PLtetO-1, output promoter PdcuS77

   Depositor

   Jeffrey Tabor

   Article

   Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

   Inserts

   narL (narL )
   sfgfp
   tetR
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPKm-243

   Plasmid

   #90506

   Purpose

   mOrange and mitochondrial-tagged sfGFP (pSIN-OFP-mitosfGFP)

   Depositor

   Todd Coleman

   Article

   Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

   Insert

   mOrange
   

   Use

   Lentiviral

   Available Since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. Reckleen_3_multieditdropout

   Plasmid

   #233458

   Purpose

   Level 2 RECKLEEN plasmid, genome editing of Klebsiella pneumoniae

   Depositor

   Anke Becker

   Article

   Elsayed et al Commun Biol. 2025 Oct 30;8(1):1509. doi:

   Insert

   Level 2 RECKLEEN plasmid, Ptac lambda Red operon, Ptet SpG cas9, J23117 acrIIA4
   

   Use

   CRISPR

   Available Since

   April 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKD250.2

   Plasmid

   #125077

   Purpose

   Expression of torR under PLtetO-1, output promoter PtorCAD129

   Depositor

   Jeffrey Tabor

   Article

   Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

   Inserts

   torR (torR )
   sfgfp
   tetR
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSR300.3

   Plasmid

   #125079

   Purpose

   Expression of uhpA under PLtetO-1, output Promoter PuhpT99

   Depositor

   Jeffrey Tabor

   Article

   Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

   Inserts

   uhpA (uhpA )
   sfgfp
   tetR
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   May 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. PermaPhos Kit

   Plasmid Kit

   #1000000226

   Purpose

   This kit is used for expressing recombinant proteins with site-specific, non-hydrolyzable phosphoserine (nhpSer) in an engineered self-sufficient E. coli genetic code expansion (GCE) expression system

   Depositor

   Ryan Mehl

   Available Since

   Sept. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

9. pPlatTET-gRNA2

   Plasmid

   #82559

   Purpose

   All in one vector which contains Cas9 peptide array (linker length: 22aa), antibody-sfGFP-TET1CD, and gRNA expression system.

   Depositor

   Izuho Hatada

   Article

   Morita et al Nat Biotechnol. 2016 Aug 29. doi: 10.103

   Insert

   dCas9-5xPlat2AflD-P2A-scFvGCN4sfGFPTET1CD
   

   Expression

   Mammalian

   Available Since

   Sept. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pST_140_LVL2 cam

    Plasmid

    #179334

    Purpose

    NT-CRISPR plasmid for a single gRNA with spG Cas9 (near PAM-less Cas9 variant).

    Depositor

    Anke Becker

    Article

    Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

    Insert

    Ptac tfoX, Ptet spG cas9, PJ23106 acrIIA4, Ptet gRNA with sfGFP dropout
    

    Use

    CRISPR

    Mutation

    Cas9 -> SpG Cas9 (D1135L, S1136W, G1218K, E121…

    Available Since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSR358.4

    Plasmid

    #125114

    Purpose

    Expression of narL(1-159aa)-liaR(154-211aa) chimera under PLtetO-1, output Promoter PliaI121

    Depositor

    Jeffrey Tabor

    Article

    Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

    Inserts

    narL(REC)-liaR(DBD)159
    sfgfp
    tetR
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. LLP453 pEf1a-NLS-aGCN4-mCherry

    Plasmid

    #100945

    Purpose

    mCherry fusion to single change variable fragment antibody GCN4 for use in SunTag system , with 3xTy1 tag

    Depositor

    Ryan Lister

    Article

    Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

    Insert

    scFvGCN4, sfGFP, GB1, mCherry
    

    Tags

    3xTy1

    Expression

    Mammalian

    Available Since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSR357.24

    Plasmid

    #125109

    Purpose

    Expression of narL(1-159aa)-ydfI(153-213aa) chimera under PLtetO-1, output Promoter PydfJ115

    Depositor

    Jeffrey Tabor

    Article

    Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

    Inserts

    narL(REC)-ydfI(DBD)159
    sfgfp
    tetR
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHR KIF17 (1-738)

    Plasmid

    #60406

    Purpose

    Expression of truncated form of human homodimeric kinesin-2 (KIF17) amino acids 1-738

    Depositor

    Ron Vale

    Article

    Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

    Insert

    KIF17 (KIF17 Human)
    

    Use

    Lentiviral

    Tags

    GB1, Strep-Tag, and sfGFP

    Expression

    Mammalian

    Mutation

    Truncated version amino acids 1-738

    Promoter

    SFFV

    Available Since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSR357.10

    Plasmid

    #125106

    Purpose

    Expression of narL(1-131aa)-ydfI(126-213aa) chimera under PLtetO-1, output Promoter PydfJ115

    Depositor

    Jeffrey Tabor

    Article

    Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

    Inserts

    narL(REC)-ydfI(DBD)131
    sfgfp
    tetR
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a-RBP-GFP(S45C)-ZdK1

    Plasmid

    #185791

    Purpose

    Encodes green flourescent protein linked to RNA binding peptide (on N terminal end ) and Z domain fragment (variant 1) of Staphylococcal protein A (on C-terminal) via GS-linker

    Depositor

    Lizhong He

    Article

    Younas et al J Phys Chem Lett. 2020 Oct 15;11(20):864

    Insert

    RBP-sfGFP(S45C)-ZdK1
    

    Tags

    10xHis, TEV protease cleavage site after His Tag

    Expression

    Bacterial

    Mutation

    Ser to Cys mutation at start of GFP, residue 45 i…

    Promoter

    T7

    Available Since

    July 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSR284

    Plasmid

    #125093

    Purpose

    Expression of ompR(1-122aa)-manR(120-234aa) chimera under PLtetO-1, output Promoter PmntCAB143

    Depositor

    Jeffrey Tabor

    Article

    Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

    Inserts

    ompR(REC)-manR(DBD)122
    sfgfp
    tetR
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSR241

    Plasmid

    #125091

    Purpose

    Expression of ompR(1-137aa)-yxdJ(132-229aa) chimera under PLtetO-1, output Promoter PyxdL181

    Depositor

    Jeffrey Tabor

    Article

    Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

    Inserts

    ompR(REC)-yxdJ(DBD)137
    sfgfp
    tetR
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKD279

    Plasmid

    #125099

    Purpose

    Expression of SO_4388(1-130aa)-psdR(134-237aa), ompR137 chimera under PLtetO-1, output Promoter PpsdA110

    Depositor

    Jeffrey Tabor

    Article

    Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

    Inserts

    SO_4388(REC)-psdR(DBD)137
    sfgfp
    tetR
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    May 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSR357.9

    Plasmid

    #125105

    Purpose

    Expression of narL(1-129aa)-ydfI(124-213aa) chimera under PLtetO-1, output Promoter PydfJ115

    Depositor

    Jeffrey Tabor

    Article

    Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

    Inserts

    narL(REC)-ydfI(DBD)129
    sfgfp
    tetR
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    May 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge