Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 14,470 results for: shi

Showing: 1461 - 1480 of 14470 results

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pAAV-CAGGS-Flex/3'USS(553)-GFP

Plasmid

#197888

Purpose

Can be used to generate AAV virus that will express GFP in the presence of Cre including the shortage of a unilateral spacer sequence(USS)

Depositor

Kazuto Kobayashi

Article

Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

Insert

GFP

Use

AAV

Mutation

N/A

Promoter

CAG

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAGGS-Flex/3'USS(285)-GFP

Plasmid

#197889

Purpose

Can be used to generate AAV virus that will express GFP in the presence of Cre including the shortage of a unilateral spacer sequence(USS)

Depositor

Kazuto Kobayashi

Article

Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

Insert

GFP

Use

AAV

Mutation

N/A

Promoter

CAG

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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eSpCas9-EF-5Usp5

Plasmid

#174873

Purpose

CRISPR vector for generating Usp5 STREAMING-tag KI cell

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

sgRNA for mouse Usp5 (Usp5 Synthetic)

Use

CRISPR

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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eSpCas9-EF-5Wnk1

Plasmid

#174874

Purpose

CRISPR vector for generating Wnk1 STREAMING-tag KI cell

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

sgRNA for mouse Wnk1 (Wnk1 Synthetic)

Use

CRISPR

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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PB-TA-ERN-ANKRD11

Plasmid

#236615

Purpose

Expresses human ANKRD11 in mammalian cells

Depositor

Tadashi Nakagawa

Article

Sashiyama et al iScience. 2025 May 19;28(6):112699. doi:

Insert

ANKRD11 (ANKRD11 Human)

Use

Piggybac transposon

Expression

Mammalian

Available Since

Oct. 24, 2025

Availability

Academic Institutions and Nonprofits only

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PB-TA-ERN-SETD5

Plasmid

#236616

Purpose

Expresses human SETD5 in mammalian cells

Depositor

Tadashi Nakagawa

Article

Sashiyama et al iScience. 2025 May 19;28(6):112699. doi:

Insert

SETD5 (SETD5 Human)

Use

Piggybac transposon

Expression

Mammalian

Available Since

Sept. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-puro-9xmyc-WDR5

Plasmid

#236614

Purpose

Expresses human WDR5 in mammalian cells

Depositor

Tadashi Nakagawa

Article

Sashiyama et al iScience. 2025 May 19;28(6):112699. doi:

Insert

WDR5 (WDR5 Human)

Tags

9xmyc

Expression

Mammalian

Available Since

Sept. 18, 2025

Availability

Academic Institutions and Nonprofits only

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PB-TA-ERN-WDR5-FLAG

Plasmid

#236721

Purpose

Expresses human WDR5 in mammalian cells

Depositor

Tadashi Nakagawa

Article

Sashiyama et al iScience. 2025 May 19;28(6):112699. doi:

Insert

WDR5 (WDR5 Human)

Use

Piggybac transposon

Tags

FLAG

Expression

Mammalian

Available Since

June 2, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-Flex/3'USS-ChRWR/Venus(ATG mut)

Plasmid

#197891

Purpose

Can be used to generate AAV virus that will express ChRWR/Venus in the presence of Cre and the leakage expression is significantly reduced without Cre

Depositor

Kazuto Kobayashi

Article

Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

Insert

ChRWR/Venus

Use

AAV

Mutation

N/A

Promoter

EF1a

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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eSpCas9-EF-5Flnc

Plasmid

#174870

Purpose

CRISPR vector for generating Flnc STREAMING-tag KI cell

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

sgRNA for mouse Flnc (Flnc Synthetic)

Use

CRISPR

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-Flex/3'USS-hM4D/2A/GFP(ATG mut)

Plasmid

#197892

Purpose

Can be used to generate AAV virus that will express hM4D/2A/GFP in the presence of Cre and the leakage expression is significantly reduced without Cre

Depositor

Kazuto Kobayashi

Article

Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

Insert

hM4D/2A/GFP

Use

AAV

Mutation

N/A

Promoter

EF1a

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-puro-mAnkrd11 (intron 4)

Plasmid

#236609

Purpose

Knockouts Ankrd11 in mouse cells

Depositor

Tadashi Nakagawa

Article

Sashiyama et al iScience. 2025 May 19;28(6):112699. doi:

Insert

Ankrd11 (Ankrd11 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-puro-3xFLAG-ANKRD11-3xHA fragment 6

Plasmid

#236623

Purpose

Expresses human ANKRD11 fragment in mammalian cells

Depositor

Tadashi Nakagawa

Article

Sashiyama et al iScience. 2025 May 19;28(6):112699. doi:

Insert

ANKRD11 (ANKRD11 Human)

Tags

3xFLAG and 3xHA

Expression

Mammalian

Mutation

aa 1831-2663

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-puro-3xFLAG-ANKRD11-3xHA fragment 2

Plasmid

#236619

Purpose

Expresses human ANKRD11 fragment in mammalian cells

Depositor

Tadashi Nakagawa

Article

Sashiyama et al iScience. 2025 May 19;28(6):112699. doi:

Insert

ANKRD11 (ANKRD11 Human)

Tags

3xFLAG and 3xHA

Expression

Mammalian

Mutation

aa 1-1006

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-puro-hANKRD11 (exon 5-2)

Plasmid

#236613

Purpose

Knockouts ANKRD11 in human cells

Depositor

Tadashi Nakagawa

Article

Sashiyama et al iScience. 2025 May 19;28(6):112699. doi:

Insert

ANKRD11 (ANKRD11 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-puro-3xFLAG-ANKRD11-3xHA fragment 1

Plasmid

#236618

Purpose

Expresses human ANKRD11 fragment in mammalian cells

Depositor

Tadashi Nakagawa

Article

Sashiyama et al iScience. 2025 May 19;28(6):112699. doi:

Insert

ANKRD11 (ANKRD11 Human)

Tags

3xFLAG and 3xHA

Expression

Mammalian

Mutation

aa 1-658

Available Since

Sept. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-puro-hANKRD11 (exon 5-1)

Plasmid

#236612

Purpose

Knockouts ANKRD11 in human cells

Depositor

Tadashi Nakagawa

Article

Sashiyama et al iScience. 2025 May 19;28(6):112699. doi:

Insert

ANKRD11 (ANKRD11 Human)

Use

CRISPR

Expression

Mammalian

Available Since

June 6, 2025

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-puro-mAnkrd11 (intron 3)

Plasmid

#236608

Purpose

Knockouts Ankrd11 in mouse cells

Depositor

Tadashi Nakagawa

Article

Sashiyama et al iScience. 2025 May 19;28(6):112699. doi:

Insert

Ankrd11 (Ankrd11 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

June 2, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-U6-DCK-Hygro

Plasmid

#202409

Purpose

gRNA expression vector for DCK

Depositor

Morito Kurata

Article

Sugita et al Commun Biol. 2023 Jun 1;6(1):582. doi: 1

Insert

guide RNA (DCK Human)

Use

CRISPR and Lentiviral

Tags

Dck

Promoter

U6

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pS97splitRBP_S1dC2

Plasmid

#183769

Purpose

Expresses SHIP1-delta-C2-Enzyme domain flanked by RBP

Depositor

William Kerr

Article

Sudan et al Front Immunol. 2022 May 4;13:830961. doi

Insert

SHIP1deltaC2 (INPP5D Human)

Tags

6HIS, RBP (C-terminal) w/HRV3C cleavage site, and…

Expression

Bacterial

Mutation

SHIP1deltaC2 includes aa N393 to aa Q730 only

Promoter

T7lac

Available Since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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