We narrowed to 1,247 results for: ncl
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Plasmid#113118PurposeDonor plasmid for knock-in T2A-H2B-Halo to the c-terminal of mouse Dppa1 coding sequenceDepositorInsertDppa1 donor region including homology arms and T2A-H2B-Halo
UseSynthetic BiologyAvailable SinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCXLE-dCas9GFP-shP53
Plasmid#102906PurposeEBNA episome plasmid for CAG promoter constitutive expression of dCas9-GFP. Includes p53 shRNA expression cassette.DepositorInsertdCas9GFP-shP53
UseCRISPRExpressionMammalianMutationD10A, H840APromoterCAGAvailable SinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
CD44I-c001
Plasmid#175448PurposeProtein expression in bacterial cells (as inclusion bodies). CD44 Link module, M63-N172.DepositorAvailable SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLY134
Plasmid#130962PurposeEngineered sgRNA-LEB5 generator including Anderson promoter J23100 for golden gate assemblyDepositorInsertsgRNA-LEB5
UseSynthetic BiologyExpressionBacterialPromoterAnderson promoter J23100Available SinceSept. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
TR-5'UTR-HP-GFP
Plasmid#80594PurposeExpresses GFP from the CBA promoter. Includes a Csy4 target hairpin in the 5'UTR for Csy4-mediated regulation. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.DepositorInsertGFP
UseAAVExpressionMammalianMutationInserted 30 nt hairpin which is targeted by Csy4 …PromoterCBAAvailable SinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
psecBFP2
Plasmid#62230Purposeencodes an inert blue fluorescent protein for use in oxidizing cellular environments including the periplasm and mammalian secretory pathway.DepositorInsertsecBFP2
Tagssecbfp2ExpressionMammalianPromotercmvAvailable SinceJuly 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
UAS::PTPRDb-DN-GFP
Plasmid#37112DepositorInsertUAS::PTPRDb-DN-GFP (ptprdb Zebrafish)
UseTol2 gateway destination vectorTagsEGFPMutationdeleted cytodomain, including phosphatase domains…PromoterUASAvailable SinceJuly 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pJLI-SUP35 (delta)2-5
Plasmid#1138DepositorInsertsup35 delta 2-5 mutant, 5' and 3' flanking regions (SUP35 Budding Yeast)
ExpressionYeastMutationlast four repeats of sup35 N domain deleted, but …Available SinceMay 25, 2005AvailabilityAcademic Institutions and Nonprofits only -
pJUMP18-cat_TU
Plasmid#127051PurposeBasic Part Whole TU; Codes for chloramphenicol resistance; including promoter and terminator. Broad host range. From Bacillus/E.coli shuttle pSEVA3b61.DepositorInsertPart Cat_TU
UseSynthetic BiologyExpressionBacterialMutationNoneAvailable SinceJuly 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLSLZ
Plasmid#51495PurposepLSL with Zif268:FokI coding sequence followed by approximately 3kb 'filler' sequence, Rep is not included on this plasmidDepositorInsertsZif268:FokI
3kb filler sequence
UsePlant t-dna plasmidTagsSV40 NLSPromotervirion-sense LIR and 2x35SAvailable SinceMay 1, 2014AvailabilityAcademic Institutions and Nonprofits only