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We narrowed to 7,508 results for: ef1a

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Showing: 2221 - 2250 of 7508 results

  1. lenti-UCOE-dCas9-BFP-Zim3-KRAB

    Plasmid
    #196716
    Purpose
    CRISPR-inhibition. dCas9-KRAB(ZIM3) with TagBFP for sorting. Improved KRAB domain from ZIM3
    Depositor
    Bernhard Schmierer
    Insert
    dCas9-TagBFP-KRAB(ZIM3)
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Mutation
    D10A, N863A
    Promoter
    EF1a
    Available Since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196716-plasmid-map-sequence-id-388040

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  2. Limk1_pLVX-EF1-Cry2-mCherry

    Plasmid
    #64368
    Purpose
    lentiviral expression of Link1 and Cry2-mCherry
    Depositor
    Matthieu Piel
    Insert
    Limk1 (LIMK1 Human)
    Use
    Lentiviral
    Tags
    Cry2-mCherry
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    April 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    64368-plasmid-map-sequence-id-486657

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  3. pLV V5-lamin B2

    Plasmid
    #175109
    Purpose
    Lentiviral expression of V5-tagged mouse Lmnb2
    Depositor
    Larry Gerace
    Insert
    Lmnb2 (Lmnb2 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Available Since
    Jan. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    175109-plasmid-map-sequence-id-351741

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  4. pLV V5-Sun2

    Plasmid
    #175114
    Purpose
    Lentiviral expression of V5-tagged mouse Sun2
    Depositor
    Larry Gerace
    Insert
    Sun2 (Sun2 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Available Since
    Jan. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    175114-plasmid-map-sequence-id-351772

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  5. miniTurbo-dCasRx

    Plasmid
    #219821
    Purpose
    EF1α-driven expression of miniTurbo fused to dCasRx with GFP (Adapted from plasmid #153209)
    Depositor
    Philipp Maass
    Insert
    dCasRx
    Use
    CRISPR
    Tags
    miniTurbo
    Expression
    Mammalian
    Promoter
    EF1a and hU6
    Available Since
    July 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    219821-plasmid-map-sequence-id-440859

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  6. pLV Erh-V5

    Plasmid
    #175123
    Purpose
    Lentiviral expression of mouse Erh-V5
    Depositor
    Larry Gerace
    Insert
    Erh (Erh Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Available Since
    Jan. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    175123-plasmid-map-sequence-id-351935

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  7. pLV_Hygro_mini-mAgrin-Myc-His

    Plasmid
    #198130
    Purpose
    Express mini-Agrin in mammalian cells
    Depositor
    Jeffrey Miner
    Insert
    mini-Agrin (Agrn Mouse)
    Use
    Lentiviral
    Tags
    TEV-Myc-His
    Promoter
    EF1a
    Available Since
    June 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    198130-plasmid-map-sequence-id-395039

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  8. pSBbi-Pur-Lyn-AktAR2-EV

    Plasmid
    #125199
    Purpose
    SB-transposon plasmid for stable expression of lipid raft-targeted Lyn-AktAR2-EV variant of FRET-based AKT activity reporter
    Depositor
    Eric Davis
    Insert
    Lyn-AktAR2-EV
    Use
    Transposon
    Tags
    EV linker, cpVenus[E172] and Lyn tag (GCIKSKRKD),…
    Expression
    Mammalian
    Promoter
    EF1a/RPBSA
    Available Since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    125199-plasmid-map-sequence-id-243587

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  9. ELL3 Streptag CtoA

    Plasmid
    #127283
    Purpose
    Expresses mouse ELL3 with streptactin tag; To interrogate if the mutation decreases putative Myc activity
    Depositor
    Christine Milcarek
    Insert
    ELL3 (NM_145973) (ELL3 Mouse)
    Tags
    Twin strep-tag
    Expression
    Mammalian
    Mutation
    CEYLHNK to AEYLHQK
    Promoter
    EF1a
    Available Since
    Aug. 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    127283-plasmid-map-sequence-id-250049

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  10. ELL1 Streptag

    Plasmid
    #127278
    Purpose
    Expresses mouse ELL1 with streptactin tag in mammalian cells
    Depositor
    Christine Milcarek
    Insert
    ELL1 (NM_007924) (ELL Mouse)
    Tags
    Twin strep-tag
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    July 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    127278-plasmid-map-sequence-id-250019

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  11. gCBEv3

    Plasmid
    #220618
    Purpose
    vector for encoding an engineered glycosylase-based cytosine (C) base editor (gCBEv3) driven by EF1a promoter, guide RNAs compatible with SpG driven by hU6, mCherry driven by CBh promoter
    Depositor
    Huawei Tong
    Article
    Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:
    Insert
    nSpCas9(Nterm)-XTEN-hUNG2-XTEN-Cas9(Cterm)
    Expression
    Mammalian
    Mutation
    hUNG2(del1-88;N213D,K184A,A214V), SpCas9(D10A)
    Promoter
    hU6, EF1a
    Available Since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    220618-plasmid-map-sequence-id-436973

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  12. gTBEv5

    Plasmid
    #220621
    Purpose
    vector for encoding an engineered glycosylase-based thymine (T) base editor (gTBEv5) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter
    Depositor
    Huawei Tong
    Article
    Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:
    Insert
    nSpCas9(Nterm)-XTEN-hUNG2-XTEN-Cas9(Cterm)
    Expression
    Mammalian
    Mutation
    hUNG2(del1-88;Y156A,A214T,Q259A,Y284D), SpCas9(D1…
    Promoter
    hU6, EF1a
    Available Since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    220621-plasmid-map-sequence-id-436953

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  13. gTBEv4

    Plasmid
    #220620
    Purpose
    vector for encoding an engineered glycosylase-based thymine (T) base editor (gTBEv4) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter
    Depositor
    Huawei Tong
    Article
    Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:
    Insert
    nSpCas9(Nterm)-XTEN-hUNG2-XTEN-Cas9(Cterm)
    Expression
    Mammalian
    Mutation
    hUNG2(del1-88;Y156A,A214T,Q259A,Y284D), SpCas9(D1…
    Promoter
    hU6, EF1a
    Available Since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    220620-plasmid-map-sequence-id-436970

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  15. pEF-GalNAcT2-msGFP2

    Plasmid
    #218965
    Purpose
    Expresses full length GalNAcT2-msGFP2 mammalian cells
    Depositor
    Benjamin Glick
    Insert
    GalNAcT2-msGFP2 (GALNT2 Human)
    Tags
    msGFP2
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Sept. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    218965-plasmid-map-sequence-id-446216

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  16. pLV V5-lamin B1

    Plasmid
    #175108
    Purpose
    Lentiviral expression of V5-tagged mouse Lmnb1
    Depositor
    Larry Gerace
    Insert
    Lmnb1 (Lmnb1 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Available Since
    Jan. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    175108-plasmid-map-sequence-id-351795

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  17. pAAV hSyn-Flex RG-cerulean

    Plasmid
    #98221
    Purpose
    Cre dependent Cerulean-E2A-SADB19 rabies glycoprotein expression, under hSyn promoter, for producing AAV virus
    Depositor
    Troy Margrie
    Insert
    Cerulean-E2A-RG
    Use
    AAV
    Tags
    cerulean
    Promoter
    human Synapsin
    Available Since
    Aug. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    98221-plasmid-map-sequence-id-192715

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  18. pEF-GalNAcT2-moxGFP2

    Plasmid
    #218966
    Purpose
    Expresses full length GalNAcT2-moxGFP2 mammalian cells
    Depositor
    Benjamin Glick
    Insert
    GalNAcT2-moxGFP2 (GALNT2 Human)
    Tags
    moxGFP2
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Sept. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    218966-plasmid-map-sequence-id-445673

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  19. pLV Agpat2-V5

    Plasmid
    #175159
    Purpose
    Lentiviral expression of mouse Agpat2-V5
    Depositor
    Larry Gerace
    Insert
    Agpat2 (Agpat2 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Available Since
    Jan. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    175159-plasmid-map-sequence-id-352271

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  20. pLV Cnep1r1-V5

    Plasmid
    #175133
    Purpose
    Lentiviral expression of mouse Cnep1r1-V5
    Depositor
    Larry Gerace
    Insert
    Cnep1r1 (Cnep1r1 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Available Since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    175133-plasmid-map-sequence-id-352020

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  21. pLV H2afy-V5

    Plasmid
    #175122
    Purpose
    Lentiviral expression of mouse Macroh2a1-V5
    Depositor
    Larry Gerace
    Insert
    Macroh2a1 (Macroh2a1 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Available Since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175122-plasmid-map-sequence-id-351930

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  22. pSBbi-Puro_LPAR_AKAR-mut

    Plasmid
    #125207
    Purpose
    SB-transposon plasmid for stable expression of unphosphorylatable "dead" version of lipid raft-targeted FRET-based PKA activity reporter
    Depositor
    Eric Davis
    Insert
    LPAR_AKAR-mut
    Use
    Transposon
    Tags
    EV linker, cpVenus[E172] and Lyn tag (GCIKSKRKD),…
    Expression
    Mammalian
    Mutation
    FoxO1 Thr-24 changed to Val rendering this inacti…
    Promoter
    EF1a/RPBSA
    Available Since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    125207-plasmid-map-sequence-id-243606

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  23. pLV V5-Sun1

    Plasmid
    #175113
    Purpose
    Lentiviral expression of V5-tagged mouse Sun1
    Depositor
    Larry Gerace
    Insert
    Sun1 (Sun1 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Available Since
    Jan. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    175113-plasmid-map-sequence-id-351771

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  24. pAAV-synmiR-10-mRuby-Pro-EGFP-8×8

    Plasmid
    #218260
    Purpose
    Expresses EGFP in mammalian cells regulated by a synthetic microRNA-based dosage compensation circuit
    Depositor
    Michael Elowitz
    Insert
    synPolyA-synmiR10-mRuby-EF1a-CMVe_MeP-EGFP-MRE10_8×8
    Use
    AAV and Synthetic Biology
    Expression
    Mammalian
    Available Since
    Dec. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    218260-plasmid-map-sequence-id-494333

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  25. pAAV-synmiR-10-mRuby-Pro-EGFP-8×9

    Plasmid
    #218261
    Purpose
    Expresses EGFP in mammalian cells regulated by a synthetic microRNA-based dosage compensation circuit
    Depositor
    Michael Elowitz
    Insert
    synPolyA-synmiR10-mRuby-EF1a-CMVe_MeP-EGFP-MRE10_8×9
    Use
    AAV and Synthetic Biology
    Expression
    Mammalian
    Available Since
    Dec. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    218261-plasmid-map-sequence-id-494523

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  26. pAAV-synmiR-L-mRuby-Pro-EGFP-4×17

    Plasmid
    #218262
    Purpose
    Expresses EGFP in mammalian cells regulated by a synthetic microRNA-based dosage compensation circuit
    Depositor
    Michael Elowitz
    Insert
    synPolyA-synmiRL-mRuby-EF1a-CMVe_MeP-EGFP-MREL_4×17
    Use
    AAV and Synthetic Biology
    Expression
    Mammalian
    Available Since
    Dec. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    218262-plasmid-map-sequence-id-494309

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  27. pAAV-synmiR-4-mRuby-Pro-EGFP-4×8

    Plasmid
    #218237
    Purpose
    Expresses EGFP in mammalian cells regulated by a synthetic microRNA-based dosage compensation circuit
    Depositor
    Michael Elowitz
    Insert
    synPolyA-synmiR4-mRuby-EF1a-CMVe_MeP-EGFP-MRE4_4×8
    Use
    AAV and Synthetic Biology
    Expression
    Mammalian
    Available Since
    Dec. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    218237-plasmid-map-sequence-id-494335

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  28. pAAV-synmiR-4-mRuby-Pro-EGFP-8×8

    Plasmid
    #218238
    Purpose
    Expresses EGFP in mammalian cells regulated by a synthetic microRNA-based dosage compensation circuit
    Depositor
    Michael Elowitz
    Insert
    synPolyA-synmiR4-mRuby-EF1a-CMVe_MeP-EGFP-MRE4_8×8
    Use
    AAV and Synthetic Biology
    Expression
    Mammalian
    Available Since
    Dec. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    218238-plasmid-map-sequence-id-494305

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  29. pAAV-synmiR-4-mRuby-Pro-EGFP-8×9

    Plasmid
    #218239
    Purpose
    Expresses EGFP in mammalian cells regulated by a synthetic microRNA-based dosage compensation circuit
    Depositor
    Michael Elowitz
    Insert
    synPolyA-synmiR4-mRuby-EF1a-CMVe_MeP-EGFP-MRE4_8×9
    Use
    AAV and Synthetic Biology
    Expression
    Mammalian
    Available Since
    Dec. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    218239-plasmid-map-sequence-id-494319

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  30. pAAV-synmiR-5-mRuby-Pro-EGFP-4×8

    Plasmid
    #218240
    Purpose
    Expresses EGFP in mammalian cells regulated by a synthetic microRNA-based dosage compensation circuit
    Depositor
    Michael Elowitz
    Insert
    synPolyA-synmiR5-mRuby-EF1a-CMVe_MeP-EGFP-MRE5_4×8
    Use
    AAV and Synthetic Biology
    Expression
    Mammalian
    Available Since
    Dec. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    218240-plasmid-map-sequence-id-494332

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Showing: 2221 - 2250 of 7508 results