We narrowed to 2,678 results for: D10

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Tad-CBE(GCA1)

Plasmid

#235075

Purpose

Expresses Tad-CBE(GCA1) in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE(GCA1)-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, bpNLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pScI_dCas9-CDA_J23119-sgRNA

Plasmid

#108550

Purpose

Bacterial Target-AID vector with sgRNA

Depositor

Akihiko Kondo

Article

Banno et al Nat Microbiol. 2018 Feb 5. pii: 10.1038/

Inserts

dCas9-PmCDA1
Streptococcus pyogenes sgRNA

Tags

Flag

Expression

Bacterial

Mutation

D10A and H840A for SpCas9

Promoter

J23119 and lambda OR

Available Since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pSSBIO32_dCas9_RPA1786

Plasmid

#240489

Purpose

CRISPRi-mediated Knockdown of RPA1786

Depositor

Rajib Saha

Article

Kathol et al Appl Environ Microbiol. 2025 Aug 20;91(8

Insert

Cas9 (cas9 Streptococcus pyogenes)

Use

CRISPR

Expression

Bacterial

Mutation

D10A, H840A

Promoter

Plac

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE EF1α dCas9-KRAB

Plasmid

#50919

Purpose

Constitutive dCas9-KRAB 2nd generation lentiviral expression vector

Depositor

Rene Maehr, Scot Wolfe

Article

Kearns et al Development. 2014 Jan;141(1):219-23. doi

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

3xHA and KRAB

Expression

Mammalian

Mutation

D10A, H840A

Promoter

EF1alpha

Available Since

March 28, 2014

Availability

Academic Institutions and Nonprofits only

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pRDA_256

Plasmid

#158581

Purpose

Lentiviral expression of BE3.9max and customizable gRNA

Depositor

John Doench, David Root

Article

Hanna et al Cell. 2021 Feb 18;184(4):1064-1080.e20.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Mutation

Cas9 D10A

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Tet1

Plasmid

#60938

Purpose

Expresses Tet1 in mammalian cells

Depositor

Yi Zhang

Article

Wang et al Cell Rep. 2014 Jan 30;6(2):278-84. doi:

Insert

Ten-Eleven Translocation 1 (Tet1 Mouse)

Tags

flag

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 10, 2015

Availability

Academic Institutions and Nonprofits only

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Lenti-EF1alpha-VP64-dCas9-VP64_Blast

Plasmid

#192650

Purpose

3rd generation lenti vector encoding VP64-dCas9-VP64 with 2A Blast resistance marker

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

VP64-dCas9-VP64

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A and N863A in Cas9

Promoter

EF1a

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pdCas9-J23111

Plasmid

#113149

Purpose

Plasmid constitutively expressing dCas9 protein

Depositor

Chong Zhang

Article

Wang et al Nat Commun. 2018 Jun 26;9(1):2475. doi:

Insert

dCas9

Expression

Bacterial

Mutation

D10A & H840A

Available Since

Jan. 6, 2020

Availability

Academic Institutions and Nonprofits only

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Lenti-EF1alpha-dCas9-p300_Blast

Plasmid

#192653

Purpose

3rd generation lenti vector encoding dCas9-p300 with 2A Blast resistance marker

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

dCas9-p300

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A; H840A in Cas9

Promoter

EF1a

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV4-p100

Plasmid

#23287

Depositor

Warner Greene

Article

Beraud et al Mol Cell Biol. 1994 Feb . 14(2):1374-82.

Insert

NFkB p100 subunit (NFKB2 Human)

Expression

Mammalian

Available Since

March 24, 2010

Availability

Academic Institutions and Nonprofits only

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ePPE-spG

Plasmid

#183096

Purpose

For plant prime editing in rice plants or monocotyledons protoplasts

Depositor

Caixia Gao

Article

Zong et al Nat Biotechnol. 2022 Mar 24. doi: 10.103

Insert

Nls-nspGCas9-NC-nls-MLV-Nls

Use

CRISPR

Expression

Plant

Mutation

D10A in SpGCas9

Available Since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

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pMJ825

Plasmid

#39315

Depositor

Jennifer Doudna

Article

Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

Insert

Cas9

Use

CRISPR

Tags

His6 and MBP

Expression

Bacterial

Mutation

Aspartate 10 to Alanine (D10A)

Promoter

T7

Available Since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only

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pdCas9-Tet1-CD

Plasmid

#83340

Purpose

To introduce dCas9-fused Tet1-CD and sgRNA2.0 system

Depositor

Ronggui Hu

Article

Xu et al Cell Discov. 2016 May 3;2:16009. doi: 10

Insert

dCas9-Tet1-CD and sgRNA scaffold with 2xMS2 binding sites

Use

CRISPR

Expression

Mammalian

Mutation

D10A, H840A

Promoter

U6, CBH

Available Since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pScI_dCas9-CDA-UL

Plasmid

#108551

Purpose

Bacterial Target-AID UGI-LVA vector

Depositor

Akihiko Kondo

Article

Banno et al Nat Microbiol. 2018 Feb 5. pii: 10.1038/

Insert

dCas9-PmCDA1-UGI-LVA

Tags

Flag

Expression

Bacterial

Mutation

D10A and H840A for SpCas9

Promoter

lambda OR

Available Since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

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Tad-CBE(TCA3)

Plasmid

#235064

Purpose

Expresses Tad-CBE(TCA3) in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE(TCA3)-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, bpNLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV4-p52

Plasmid

#23289

Depositor

Warner Greene

Article

Beraud et al Mol Cell Biol. 1994 Feb . 14(2):1374-82.

Insert

p52 (NFKB2 Human)

Expression

Mammalian

Available Since

March 31, 2010

Availability

Academic Institutions and Nonprofits only

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pHAGE EF1α dCas9-VP64

Plasmid

#50918

Purpose

Constitutive dCas9-VP64 lentiviral expression vector

Depositor

Rene Maehr, Scot Wolfe

Article

Kearns et al Development. 2014 Jan;141(1):219-23. doi

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

3xHA and VP64

Expression

Mammalian

Mutation

D10A, H840A

Promoter

EF1alpha

Available Since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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TLR4

Plasmid

#20863

Depositor

Jie-Oh Lee

Article

Park et al Nature. 2009 Apr 30;458(7242):1191-5. do

Insert

Toll-Like Receptor 4 (TLR4 Human)

Expression

Insect

Mutation

Contains ectodomain (amino acids 27–631)

Available Since

May 20, 2009

Availability

Academic Institutions and Nonprofits only

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pCT310

Plasmid

#111140

Purpose

Bacterial expression of 6His-dCas9-3XFlag

Depositor

Robert Tjian

Article

Tsui et al Proc Natl Acad Sci U S A. 2018 Mar 20;11

Insert

dCas9

Tags

3X Flag and 6X His

Expression

Bacterial

Mutation

D10A - H840A

Promoter

T7

Available Since

July 12, 2018

Availability

Academic Institutions and Nonprofits only

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pH-A3A-PBE

Plasmid

#119774

Purpose

Conversion of C to T in rice plants transformation

Depositor

Caixia Gao

Article

Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

Insert

APOBEC3A-nCas9-NLS-UGI-NLS

Tags

NLS (flanking UGI)

Expression

Plant

Mutation

nCas9 has a mutation D10A in wild type SpCas9

Promoter

maize Ubiquitin-1

Available Since

Sept. 26, 2019

Availability

Academic Institutions and Nonprofits only

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