We narrowed to 11,139 results for: Kars

1. pCAG-OSF-VPS34

   Plasmid

   #99327

   Purpose

   Component for mammalian expression of recombinant PI3KC3-C1 complex

   Depositor

   James Hurley

   Article

   Baskaran et al Elife. 2014 Dec 9;3. doi: 10.7554/eLife.

   Insert

   VPS34 (PIK3C3 Human)
   

   Use

   Tags

   Strep-Strep-Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

2. HPF1-c004

   Plasmid

   #216874

   Purpose

   Bacterial expression vector for E. coli producing full length HPF1 protein with N-terminal YFP fusion

   Depositor

   Lari Lehtiö

   Article

   Dhakar et al Sci Rep. 2024 Feb 16;14(1):3875. doi: 10

   Insert

   Full length HPF1 (HPF1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSLIK TT 3xFLAG SMAD4 neo

   Plasmid

   #83273

   Purpose

   Lentiviral vector for inducible expression of SMAD4 with 3xFLAG N-terminal tag

   Depositor

   Kevin Janes

   Article

   Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

   Insert

   SMAD4 (SMAD4 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

4. pLVX-opto-mRipK3

   Plasmid

   #208783

   Purpose

   Inducibly expresses mouse RipK3 fused to light activatable Cry2

   Depositor

   Petr Broz

   Article

   Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

   Insert

   mCherry mRipK3 Cry2 (Ripk3 Mouse)
   

   Use

   Lentiviral

   Tags

   Cry2 and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. Circular 100,50 (RAB7A)

   Plasmid

   #170117

   Purpose

   AAV vector carrying a guide RNA targeting the human RAB7A mRNA

   Depositor

   Prashant Mali

   Article

   Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

   Insert

   Circular 100,50 guide RNA
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6

   Available since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. Circular 200,100 with interspersed loops (SMAD4)

   Plasmid

   #180191

   Purpose

   AAV vector carrying a guide RNA targeting the human SMAD4 mRNA

   Depositor

   Prashant Mali

   Article

   Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

   Insert

   Circular 200,100 guide RNA with interspersed loops
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6

   Available since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTSK561

   Plasmid

   #190816

   Purpose

   For C-terminal -Halo Tagging of a gene of interest with URA3 selection marker in yeast S. cerevisiae

   Depositor

   Tatiana Karpova, Gunjan Mehta

   Article

   Ball et al Nucleic Acids Res. 2016 Dec 1;44(21):e16

   Insert

   HaloTag
   

   Use

   Bacterial cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. PXL

   Plasmid

   #75349

   Purpose

   PX330 derived. Bbs1 & annealed oligos to insert guide strand. BstB1+Pac1 of PXL and FUX-/pRubiX- T2A-Cas9 for choice of sgRNA, viral backbone, and fluorescent protein. Pac1 to introduce 2nd sgRNA.

   Depositor

   Bryan Luikart

   Article

   Williams et al Sci Rep. 2016 May 10;6:25611. doi: 10.10

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. SiC-V2-Cas9G7

   Plasmid

   #133043

   Purpose

   Doxycycline-inducible SiC-V2 vector with Cas9G7 (sgRNA 7 targeting SpCas9 gene). In cells coexpressing Cas9 nuclease, this construct causes self-inactivation/editing of SpCas9 gene upon Dox addition.

   Depositor

   Sriram Neelamegham

   Article

   Kelkar et al Mol Ther. 2019 Sep 12. pii: S1525-0016(1

   Insert

   Tet repressor
   

   Use

   Lentiviral

   Tags

   Cerulean

   Expression

   Mutation

   Promoter

   Available since

   Nov. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLX304 GDF11-V5 blast

    Plasmid

    #83098

    Purpose

    Lentiviral vector for constitutive expression of human GDF11 with C-terminal V5 tag

    Depositor

    Kevin Janes

    Article

    Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

    Insert

    GDF11 (GDF11 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDEINO1

    Plasmid

    #179472

    Purpose

    Multi-host shuttle plasmid for D. radiodurans, E. coli, S. cerevisiae, S. meliloti, and P. tricornutum; contains an oriT for transfer via conjugation

    Depositor

    Bogumil Karas

    Article

    Brumwell et al ACS Synth Biol. 2022 Mar 18;11(3):1068-1

    Inserts

    ori
    cat
    

    Use

    Synthetic Biology; Bacterial and yeast cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair3

    Plasmid

    #98974

    Purpose

    Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 37bp 3' overhangs. TagBFP.

    Depositor

    Jan Karlseder

    Article

    Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

    Insert

    LMNA sgRNAs pair 3 and Cas9(D10A)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcdna3 Flag JIP3b

    Plasmid

    #53458

    Purpose

    interacts with MAPK signaling module

    Depositor

    Roger Davis

    Article

    Kelkar et al Mol Cell Biol. 2000 Feb;20(3):1030-43.

    Insert

    mitogen-activated protein kinase 8 interacting protein 3 (Mapk8ip3 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pLX302 GDF11-V5 puro

    Plasmid

    #83097

    Purpose

    Lentiviral vector for constitutive expression of human GDF11 with C-terminal V5 tag

    Depositor

    Kevin Janes

    Article

    Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

    Insert

    GDF11 (GDF11 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

15. PARP1-c009

    Plasmid

    #216872

    Purpose

    Bacterial expression vector for E. coli producing PARP1 catalytic protein (without Regulatory domain); with N-terminal CFP fusion

    Depositor

    Lari Lehtiö

    Article

    Dhakar et al Sci Rep. 2024 Feb 16;14(1):3875. doi: 10

    Insert

    PARP1 catalytic domain (no Regulatory Domain) (PARP1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUSE-RapR-Src(KD)-myc

    Plasmid

    #25934

    Depositor

    Klaus Hahn

    Article

    Karginov et al Nat Biotechnol. 2010 Jul;28(7):743-7. do

    Insert

    RapR-Src (Fkbp1a Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    This pUSE-RapR-Src(KD)-myc plasmid contains the k…

    Promoter

    Available since

    Oct. 26, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSC5GGv2

    Plasmid

    #188604

    Purpose

    pSC5 Golden-Gate Cloning Plasmid (BsaI - Site 2)

    Depositor

    Bogumil Karas

    Article

    Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

    Type

    Empty backbone

    Use

    Synthetic Biology; Diatom expression

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair4

    Plasmid

    #98975

    Purpose

    Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 94bp 3' overhangs. TagBFP.

    Depositor

    Jan Karlseder

    Article

    Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

    Insert

    LMNA sgRNAs pair 4 and Cas9(D10A)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLEXm-GST-ATG14

    Plasmid

    #99329

    Purpose

    Component for mammalian expression of recombinant PI3KC3-C1 complex

    Depositor

    James Hurley

    Article

    Baskaran et al Elife. 2014 Dec 9;3. doi: 10.7554/eLife.

    Insert

    ATG14 (ATG14 Human)
    

    Use

    Tags

    GST-TEV tag

    Expression

    Mammalian

    Mutation

    Codon optimized

    Promoter

    CMV

    Available since

    Aug. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAGE2.0-i

    Plasmid

    #194639

    Purpose

    Empty pAGE2.0 plasmid with pBAD inducible promoter

    Depositor

    Bogumil Karas

    Article

    Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

    Type

    Empty backbone

    Use

    Synthetic Biology; Diatom expression

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge