We narrowed to 159,671 results for: addgene

1. KLHL40 (KBTBD5A-c006)

   Plasmid

   #98233

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   KBTBD5A (KLHL40 Human)
   

   Use

   Tags

   TEV-His6-Flag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR EGFP-guide1

   Plasmid

   #118158

   Purpose

   CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA1 against the EGFP CDS

   Depositor

   Jorge Ferrer

   Insert

   KRAB-dCas9-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a core and U6

   Available since

   April 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. 5TEY (METTL3)

   Plasmid

   #101892

   Purpose

   Baculovirus expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   METTL3 (METTL3 Human)
   

   Use

   Baculovirus expression

   Tags

   6xHis, TEV

   Expression

   Mutation

   Promoter

   polyhedrin

   Available since

   April 30, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. 4IKP

   Plasmid

   #60080

   Purpose

   Baculovirus expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   CARM1:JMC049-A06:C226836 (CARM1 Human)
   

   Use

   Baculovirus expression

   Tags

   6xHis

   Expression

   Mutation

   See comments

   Promoter

   polyhedrin

   Available since

   Oct. 22, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

5. pPB_EF1a_FLAG-tNGFR_ZeoR

   Plasmid

   #212649

   Purpose

   piggyBac vector to express FLAG-tNGFR with zeocin resistance

   Depositor

   Paul Blainey

   Article

   Najia et al bioRxiv 2024.10.01.616098

   Insert

   tNGFR
   

   Use

   Piggybac

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Nov. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. CFL1 (CFL1A-c002)

   Plasmid

   #98237

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   CFL1A (CFL1 Human)
   

   Use

   Tags

   His-6-TEV

   Expression

   Bacterial

   Mutation

   pfam00241:Cofilin_ADF 27-148

   Promoter

   T7

   Available since

   Sept. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. LRG2.1-TagBFP2

   Plasmid

   #124773

   Purpose

   Lentiviral plasmid to co-express a guide RNA and TagBFP2

   Depositor

   Jason Sheltzer

   Article

   Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. KLHL3 (KLHL3A-c003)

   Plasmid

   #110251

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   KLHL3 (KLHL3 Human)
   

   Use

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7 - lacO

   Available since

   June 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSANG14-3F

   Plasmid

   #39265

   Depositor

   John McCafferty

   Article

   Martin et al BMC Biotechnol. 2006 Dec 7;6:46.

   Type

   Empty backbone

   Use

   Tags

   6xHis, Alkaline phosphatase, pelB leader, and tri…

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pACRISPR-TP

    Plasmid

    #203589

    Purpose

    A sgRNA expression plasmid for genome editing in Burkholderia

    Depositor

    Leonilde Moreira

    Article

    Ferreira et al Appl Environ Microbiol. 2024 Feb 21;90(2

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. EdTC

    Plasmid

    #24310

    Depositor

    Roel Nusse

    Article

    Fuerer et al PLoS One. 2010 . 5(2):e9370.

    Insert

    EF1alpha-dnhTcf4 // SV40-mCherry (TCF7L2 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    31 first amino acids of hTcf4 have been deleted.

    Promoter

    Available since

    April 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. 5VC9 (CXXC4)

    Plasmid

    #101263

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    CXXC4 (CXXC4 Human)
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 31, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pHdCas9

    Plasmid

    #137080

    Purpose

    Expression of Himar-dCas9 in bacteria

    Depositor

    Harris Wang

    Article

    Chen et al CRISPR J. 2019 Dec;2(6):376-394. doi: 10

    Insert

    Himar-dCas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLtetO-1

    Available since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. KEAP1 (KEAP1A-c029)

    Plasmid

    #110247

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    KEAP1 (KEAP1 Human)
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7 - lacO

    Available since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-dSaCas9-KRAB-gRNA-TRE-blast

    Plasmid

    #201151

    Purpose

    Lentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9) fused to the KRAB domain as well as a gRNA targeting the tight TRE promoter and the blasticidin resistance gene.

    Depositor

    Moshe Szyf

    Inserts

    SadCas9-KRAB
    gRNA-TRE
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mutation

    gRNA sequence: ATCAGTGATAGAGAACGTATG

    Promoter

    Available since

    July 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pACλCI

    Plasmid

    #53730

    Purpose

    PlacUV5-directed synthesis of the λCI protein; used as two-hybrid control. For cloning purposes, see Addgene plasmid 53733.

    Depositor

    Ann Hochschild

    Article

    Dove et al Nature. 1997 Apr 10;386(6625):627-30.

    Insert

    bacteriophage lambda CI protein
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac UV5

    Available since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-CMV-FLEX-SaCas9-U6-sgKcnd3

    Plasmid

    #159912

    Purpose

    Mutagenesis of Kcnd3

    Depositor

    Larry Zweifel

    Insert

    Kcnd3 (Kcnd3 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. XLone-BSD SARS-CoV2 N P2A mCherry

    Plasmid

    #154398

    Purpose

    PiggybacTransposon-based tunable and temporal expression control of SARS-CoV2 N and mCherry

    Depositor

    Xiaoping Bao

    Insert

    SARS-CoV2 N Protein (N SARS-CoV2)
    

    Use

    Piggybac

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBabe puro IkB alpha M

    Plasmid

    #12332

    Depositor

    Inder Verma

    Insert

    IkB alpha M (Nfkbia Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Dominant-negative mutant. Many signal transductio…

    Promoter

    Available since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

20. pAG306-GPD-ccdB chr I

    Plasmid

    #41894

    Depositor

    Dan Gottschling

    Article

    Hughes et al Nature. 2012 Dec 13;492(7428):261-5. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GPD

    Available since

    Feb. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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