We narrowed to 5,055 results for: cre encoding plasmid

1. pAAV-TRE-DIO-eGFP-L10a

   Plasmid

   #166605

   Purpose

   Encodes Cre-dependent eGFP-L10a under control of the TRE

   Depositor

   Martin Myers

   Article

   Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

   Insert

   eGFP-L10a
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   tetracycline response element

   Available since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-TRE-DIO-GFP-2A-SynmRuby

   Plasmid

   #166608

   Purpose

   Encodes Cre-dependent GFP-2A-synaptophysinmRuby under control of the TRE

   Depositor

   Martin Myers

   Article

   Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

   Insert

   GFP-2A-syn
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   tetracycline response element

   Available since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-TRE-DIO-SwiChR-eYFP

   Plasmid

   #166604

   Purpose

   Encodes Cre-dependent SwiChR-eYFP under control of the TRE

   Depositor

   Martin Myers

   Article

   Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

   Insert

   SwiChR-eYFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   tetracycline response element

   Available since

   April 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMS110

   Plasmid

   #215674

   Purpose

   Split hygromycinR landing pad insertion plasmid for ChrIII

   Depositor

   Patrick Phillips

   Article

   Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

   Insert

   5'HA + synthetic guide site3 + 3'ΔHygR:unc-54 3' UTR + lox2272 + SEC + lox2272 + 3'HA
   

   Use

   CRISPR and Cre/Lox

   Tags

   Expression

   Worm

   Mutation

   3' ∆HYGR is promoterless and encodes aa60-341

   Promoter

   Available since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a T7pCONS sfGFP

   Plasmid

   #154462

   Purpose

   pET28a with a design flaw in the T7 promoter fixed. Contains the coding sequence for sfGFP

   Depositor

   Daniel Daley

   Article

   Shilling et al Commun Biol. 2020 May 7;3(1):214. doi: 1

   Insert

   super folder GFP
   

   Use

   Tags

   His-thrombin

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTS2308-Tier1-pGL3-PPGK-NLuc_TB

   Plasmid

   #169551

   Purpose

   Tier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' insulator sequence derived from the pGL3 plasmid (pGL3-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   pGL3-insulator-modulated PPGK-driven NanoLuc Luciferase - mTagBFP expression cassette
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PPGK

   Available since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV8-hSyn-flex-miR30-eGFP-shDyn

   Plasmid

   #132716

   Purpose

   Encodes Cre-dependent short hairpin RNA (shRNA) that targets the 3’-untranslated region of the rat Pdyn gene, which encodes dynorphin

   Depositor

   Robert Messing

   Article

   Pomrenze et al Cell Rep. 2019 Oct 1;29(1):13-21.e4. doi

   Insert

   GFP-shDyn (Pdyn Rat)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Synapsin 1

   Available since

   Oct. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV8-hSyn-flex-miR30-eGFP-shCrh

   Plasmid

   #132715

   Purpose

   Encodes Cre-dependent short hairpin RNA (shRNA) that targets the 3’-untranslated region of the rat Crh gene which encodes corticotropin releasing factor

   Depositor

   Robert Messing

   Article

   Pomrenze et al Cell Rep. 2019 Oct 1;29(1):13-21.e4. doi

   Insert

   GFP-shCrh (Crh Rat)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Synapsin 1

   Available since

   Oct. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-6xTRE-FLEX-hM4Di.mCherry

   Plasmid

   #169415

   Purpose

   Activity-dependent, cre-dependent, temporally restricted inhibitory DREADD expression

   Depositor

   David Talmage

   Article

   Rajebhosale et al Elife. 2024 Feb 16;13:e86581. doi: 10.75

   Insert

   hM4Di.mCherry
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mutation

   hM4Di is Gi-coupled DREADD.Derived from human mus…

   Promoter

   Minimal TATA

   Available since

   March 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDNA3-SLPI

    Plasmid

    #18103

    Depositor

    Ronny Drapkin

    Insert

    Secretory Leucocyte Protease Inhibitor (SLPI Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 11, 2008

    Availability

    Academic Institutions and Nonprofits only

11. pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE

    Plasmid

    #104495

    Purpose

    AAV-mediated expression of ultrasensitive protein calcium sensor under the CAG promoter, Cre mediated flip-excision switch

    Depositor

    GENIE Project, Douglas Kim

    Article

    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

    Has Service

    AAV PHP.eB, AAV Retrograde, and AAV1

    Insert

    jGCaMP7s
    

    Use

    AAV and Cre/Lox

    Tags

    T7 epitope, Xpress tag, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pattB-LSL-AAVR-F2A-spCas9

    Plasmid

    #202459

    Purpose

    The plasmid backbone used to generate the recombination template to generate the SELECTIV mice through Integrase Mediated Transgenesis. Allows for Cre-dependent expression of AAVR and Cas9.

    Depositor

    Jan Carette

    Article

    Zengel et al Nat Methods. 2023 Jul;20(7):1070-1081. d

    Insert

    AAVR (AU040320 Mouse)
    

    Use

    CRISPR, Cre/Lox, and Mouse Targeting

    Tags

    mCherry fusion

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGP-AAV-syn-FLEX-jGCaMP7s-WPRE

    Plasmid

    #104491

    Purpose

    AAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter, Cre mediated flip-excision switch

    Depositor

    GENIE Project, Douglas Kim

    Article

    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

    Has Service

    AAV PHP.eB, AAV Retrograde, AAV1, and AAV9

    Insert

    jGCaMP7s
    

    Use

    AAV and Cre/Lox

    Tags

    T7 epitope, Xpress tag, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGP-AAV-CAG-FLEX-jGCaMP7c variant 1513-WPRE

    Plasmid

    #105323

    Purpose

    AAV-mediated expression of ultrasensitive protein calcium sensor under the CAG promoter, Cre-dependent expression

    Depositor

    GENIE Project, Douglas Kim

    Article

    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

    Insert

    jGCaMP7c variant 1513
    

    Use

    AAV and Cre/Lox

    Tags

    T7 epitope, Xpress tag, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGP-AAV-syn-FLEX-jGCaMP7c variant 1513-WPRE

    Plasmid

    #105322

    Purpose

    AAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter, Cre-dependent expression

    Depositor

    GENIE Project, Douglas Kim

    Article

    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

    Has Service

    AAV1

    Insert

    jGCaMP7c variant 1513
    

    Use

    AAV and Cre/Lox

    Tags

    T7 epitope, Xpress tag, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-Syn-FLEX-fd [ChrimsonR-tdTomato]

    Plasmid

    #84483

    Purpose

    AAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter, in floxed/forward (Cre-dependent) manner. Cre turns gene off. Using bGHpA signal. tdTomato is a codon diversified version.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    ChrimsonR-tdTomato
    

    Use

    AAV

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Chrimson K176R mutant

    Promoter

    human synapsin promoter

    Available since

    April 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pOTTC1179 - pAAV (flox-stop) TH gRNA A EF1a eGFP

    Plasmid

    #113158

    Purpose

    An AAV vector that expresses a Cre-dependent guide RNA targeting rat TH and expresses EGFP reporter

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Insert

    gRNA for rat TH
    

    Use

    AAV, CRISPR, and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. AIO-GFP

    Plasmid

    #74119

    Purpose

    All-in-One plasmid encoding dual U6 promoter-driven sgRNAs and EGFP-coupled Cas9-D10A nickase to enhance efficient and accurate genome editing

    Depositor

    Steve Jackson

    Article

    Chiang et al Sci Rep. 2016 Apr 15;6:24356. doi: 10.10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AIO-mCherry

    Plasmid

    #74120

    Purpose

    All-in-One plasmid encoding dual U6 promoter-driven sgRNAs and mCherry-coupled Cas9-D10A nickase to enhance efficient and accurate genome editing

    Depositor

    Steve Jackson

    Article

    Chiang et al Sci Rep. 2016 Apr 15;6:24356. doi: 10.10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCCl-MCS_miniCMV_EGFP

    Plasmid

    #134984

    Purpose

    The plasmid includes a EGFP gene driven by a minimal CMV promoter. Cis-regulatory elements can be cloned into the single EcoRV site. This plasmid can be used for generating a lentiviral vector

    Depositor

    Claudio Mussolino

    Article

    Roman Azcona et al Nat Protoc. 2020 Oct;15(10):3410-3440. d

    Insert

    EGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    minCMV

    Available since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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