We narrowed to 3,816 results for: golden gate

1. pM.L6

   Plasmid

   #234592

   Purpose

   Linker plasmid (connect pMB.Pos5 to pMB.002)

   Depositor

   Chen Davidovich

   Article

   Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

   Type

   Empty backbone

   Use

   Other

   Available Since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAIP4_4QMD

   Plasmid

   #234174

   Purpose

   Heterologous protein expression of envoplakin in Escherichia coli

   Depositor

   Christopher Bahl

   Insert

   4QMD (EVPL Human)
   

   Tags

   10x HisTag-Smt3

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 6, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pAIP4_1BKR

   Plasmid

   #234168

   Purpose

   Heterologous protein expression of spectrin beta chain, non-erythrocytic 1 (Beta-II spectrin) in Escherichia coli

   Depositor

   Christopher Bahl

   Insert

   1BKR (SPTBN1 Human)
   

   Tags

   10x HisTag-Smt3

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 6, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pAIP4_2JIK

   Plasmid

   #234170

   Purpose

   Heterologous protein expression of synaptojanin-2-binding protein in Escherichia coli

   Depositor

   Christopher Bahl

   Insert

   2JIK (SYNJ2BP Human)
   

   Tags

   10x HisTag-Smt3

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 6, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pAIP4_5EF9

   Plasmid

   #234154

   Purpose

   Heterologous protein expression of polymerase basic protein 2 (RNA-directed RNA polymerase subunit P3) in Escherichia coli

   Depositor

   Christopher Bahl

   Insert

   5EF9 (PB2 Influenza B virus (B/Lee/1940))
   

   Tags

   10x HisTag-Smt3

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 6, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. Human Coxsackievirus A16 strain G10 inactive 2A protease

   Plasmid

   #228665

   Purpose

   Bacterial expression of the A71 2A protease, innactive and can be used as a control in assays

   Depositor

   ASAP Discovery, Frank von Delft

   Insert

   Enterovirus A71 2A protease
   

   Tags

   His-SUMO

   Expression

   Bacterial

   Mutation

   Avitag insertion and C2A mutations / Active site …

   Available Since

   Feb. 18, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. WNV isolate WN NY 2000-crow3356 NS5 RdRp full length

   Plasmid

   #228662

   Purpose

   Bacterial expression of the West Nile virus NS5 RdRp protein for biochemical assay

   Depositor

   ASAP Discovery, Frank von Delft

   Insert

   West nile NS5 RdRp
   

   Tags

   His-SUMO

   Expression

   Bacterial

   Available Since

   Feb. 18, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. WESSV strain SAH177 NS2B-NS3 protease fusion with non cleavable linker

   Plasmid

   #228659

   Purpose

   Bacterial expression of the fusion of the NS2B-NS3 protease from Wesselsbron virus for biochemical assay

   Depositor

   ASAP Discovery, Frank von Delft

   Insert

   Wesselsbron virus portion of NS2B protease fused to NS3
   

   Tags

   His-Twin Strep-Tev

   Expression

   Bacterial

   Available Since

   Feb. 18, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. Echovirus 11 (E11 3C) protease A isolate E11-PLA-NECK-23-E0-62-P1-FRA23

   Plasmid

   #228645

   Purpose

   Bacterial expression of the E11 3C protease

   Depositor

   ASAP Discovery, Frank von Delft

   Insert

   Echo virus 11 3C protease
   

   Tags

   His-SUMO

   Expression

   Bacterial

   Available Since

   Feb. 18, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. Coxsackievirus B4 (CVB4) 3C protease isolate CVB4/USA/9R1/2012

    Plasmid

    #228640

    Purpose

    Bacterial expression of the Coxsackievirus 43 isoform B 3C protease

    Depositor

    ASAP Discovery, Frank von Delft

    Insert

    Coxsackievirus 43 isoform B 3C protease
    

    Tags

    His-SUMO

    Expression

    Bacterial

    Available Since

    Feb. 18, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pMulti_K_ccdB

    Plasmid

    #223852

    Purpose

    GEC-acceptor for 4G-cloning, designed for expression in E. coli (Kanamyicin resistance)

    Depositor

    Stephan Gruber

    Article

    Taschner et al bioRxiv 2024.06.17.599261

    Type

    Empty backbone

    Use

    Golden-gate acceptor

    Expression

    Bacterial

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMulti_A_ccdB

    Plasmid

    #223851

    Purpose

    GEC-acceptor for 4G-cloning, designed for expression in E. coli (Ampicillin resistance)

    Depositor

    Stephan Gruber

    Article

    Taschner et al bioRxiv 2024.06.17.599261

    Type

    Empty backbone

    Use

    Golden-gate acceptor

    Expression

    Bacterial

    Available Since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. L1PB26- pAHP6--PhiC31-NLS-tUBQ1 + pGATA23--Bxb1-SAUR-tUBQ10

    Plasmid

    #219701

    Purpose

    Level 1 PhiC31 and Bxb1 integrase construct for tracking of lateral root development: pAHP6 promoter for PhiC31 and pGATA23 promoter for Bxb1, NLS tag for PhiC31 and RNA degradation tag for Bxb1

    Depositor

    Jennifer Nemhauser

    Insert

    pAHP6::PhiC31-NLS:tUBQ1 + pGATA23::Bxb1-SAUR:tUBQ10
    

    Expression

    Plant

    Available Since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. MUT_S1-H1,NR

    Plasmid

    #218395

    Purpose

    3-exon minigene, with SRSF1 motif-rich middle exon, hnRNPA1 motif-rich region in second intron, both randomly mutated to avoid sequence repeats, and AG>GG mutation in final 3' splice site

    Depositor

    Oded Regev

    Insert

    Modified CHO DHFR minigene
    

    Expression

    Mammalian

    Promoter

    tetracycline-responsive promoter

    Available Since

    May 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pD(Ct)_FLAG3

    Plasmid

    #223896

    Purpose

    4G-cloning donor for C-terminal FLAG3 tag

    Depositor

    Stephan Gruber

    Article

    Taschner et al bioRxiv 2024.06.17.599261

    Insert

    FLAG3
    

    Use

    Golden-gate donor

    Available Since

    Sept. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRDB_345

    Plasmid

    #231113

    Purpose

    ABE activity-based selection cloning vector

    Depositor

    John Doench, David Root

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRDB_347

    Plasmid

    #231114

    Purpose

    CBE activity-based selection cloning vector

    Depositor

    John Doench, David Root

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAIP4_2A4D

    Plasmid

    #234196

    Purpose

    Heterologous protein expression of ubiquitin-conjugating enzyme E2 variant 1 (UEV-1) (CROC-1) in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    2A4D (UBE2V1 Human)
    

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pAIP4_2EYI

    Plasmid

    #234173

    Purpose

    Heterologous protein expression of alpha-actinin-1 in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    2EYI (ACTN1 Human)
    

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pSG1017-2xBsaI-SaKKH-2xUGI_P2A_EGFP

    Plasmid

    #239450

    Purpose

    Codon-optimized S. aureus KKH Cas9 with 2x UGI. 2x BsaI sites for easy cloning of varying deaminases for cytosine base editing. EGFP can serve as a transfection marker.

    Depositor

    Alexis Komor

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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