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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 5,317 results for: golden gate

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Showing: 1481 - 1500 of 5317 results

  3. pLY133

    Plasmid
    #130961
    Purpose
    Engineered sgRNA-LEB4 generator including Anderson promoter J23100 for golden gate assembly
    Depositor
    Baojun Wang
    Publication
    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:
    Insert
    sgRNA-LEB4
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    Anderson promoter J23100
    Available since
    Dec. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    130961-plasmid-map-sequence-id-266878

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  4. pLY131

    Plasmid
    #130956
    Purpose
    Engineered sgRNA-LEB2 generator including Anderson promoter J23100 for golden gate assembly
    Depositor
    Baojun Wang
    Publication
    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:
    Insert
    sgRNA-LEB2
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    Anderson promoter J23100
    Available since
    Dec. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    130956-plasmid-map-sequence-id-266875

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  5. pLY132

    Plasmid
    #130960
    Purpose
    Engineered sgRNA-LEB3 generator including Anderson promoter J23100 for golden gate assembly
    Depositor
    Baojun Wang
    Publication
    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:
    Insert
    sgRNA-LEB3
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    Anderson promoter J23100
    Available since
    Sept. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    130960-plasmid-map-sequence-id-257712

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  6. pLY130

    Plasmid
    #130952
    Purpose
    Engineered sgRNA-LEB1 generator including Anderson promoter J23100 for golden gate assembly
    Depositor
    Baojun Wang
    Publication
    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:
    Insert
    sgRNA-LEB1
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    Anderson promoter J23100
    Available since
    Sept. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    130952-plasmid-map-sequence-id-257710

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  11. pAcHELIX-sgRNA_entry (CJT94)

    Plasmid
    #181783
    Purpose
    Expresses AcHELIX containing a nicking I-AniI fusion to AcTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Publication
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    nAniI-AcTnsB, AcTnsC, AcTniQ, AcCas12k
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available since
    March 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    181783-plasmid-map-sequence-id-358223

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  12. pShHELIX(Cas12k-TniQ-TniQ)-sgRNA_entry (CJT112)

    Plasmid
    #181791
    Purpose
    Expresses 3-component ShHELIX containing a Cas12k-TniQ-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Publication
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    nAniI-ShTnsB, ShTnsC, ShCas12k-ShTniQ-ShTniQ
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    181791-plasmid-map-sequence-id-354814

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  18. HSA_3b*_AP

    Plasmid
    #238899
    Purpose
    Plasmid insert contains a gene sequence for Human serum albumin for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).
    Depositor
    Joseph Brock
    Publication
    Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86
    Insert
    Recombinant Human Serum Albumin (HSA) 3b*
    Use
    Synthetic Biology
    Available since
    Sept. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    238899-plasmid-map-sequence-id-482774

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Showing: 1481 - 1500 of 5317 results