We narrowed to 7,548 results for: ski

1. pCellFree_G03 TYK2

   Plasmid

   #67133

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   TYK2 (TYK2 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Expression

   Mutation

   A435S

   Promoter

   T7

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pCellFree_G03 BMPR2

   Plasmid

   #67127

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   BMPR2 (BMPR2 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Expression

   Mutation

   Promoter

   T7

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pBABEpuro-ARAF_S214F

   Plasmid

   #51078

   Purpose

   mammalian expression of ARAF S214F

   Depositor

   Matthew Meyerson

   Article

   Imielinski et al J Clin Invest. 2014 Apr;124(4):1582-6. d

   Insert

   ARAF (ARAF Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   S214F

   Promoter

   Available since

   Feb. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-U6-DR36(DjCas13d-SapI)-CMV-intron-GFP-pA

   Plasmid

   #192500

   Purpose

   To express DjCas13d compatible gRNA and GFP

   Depositor

   Jonathan Ploski

   Article

   Back et al Mol Ther Methods Clin Dev, 20 Aug 2025,

   Insert

   DjCas13d
   

   Use

   AAV

   Tags

   Expression

   Mutation

   No

   Promoter

   CMV

   Available since

   Aug. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAV-U6-DR30(SapI)-CMV-intron-MCS-pA

   Plasmid

   #192496

   Purpose

   To express CasRX gRNA and contains MCS to insert coding region of interest

   Depositor

   Jonathan Ploski

   Article

   Back et al Mol Ther Methods Clin Dev, 20 Aug 2025,

   Insert

   CasRx
   

   Use

   AAV

   Tags

   Expression

   Mutation

   No

   Promoter

   CMV

   Available since

   Aug. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV-EFS-hfCas13d-T2A-mCherry-pA

   Plasmid

   #192497

   Purpose

   To express hfCas13d

   Depositor

   Jonathan Ploski

   Article

   Back et al Mol Ther Methods Clin Dev, 20 Aug 2025,

   Insert

   high fidelity (hf) version of CasRx (hfCas13d)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Yes: GTGgaatacattaccaacGTGGTGtacGTG

   Promoter

   EFS

   Available since

   Aug. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-U6-DR30(SapI)-EFS-CasRx-T2A-mCherry-pA

   Plasmid

   #192481

   Purpose

   To express CasRX and a gRNA

   Depositor

   Jonathan Ploski

   Article

   Back et al Mol Ther Methods Clin Dev, 20 Aug 2025,

   Insert

   CasRx
   

   Use

   AAV

   Tags

   Expression

   Mutation

   No

   Promoter

   U6/EFS

   Available since

   Aug. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3.3-3XFLAG-AGO1

   Plasmid

   #153556

   Purpose

   argonaute RISC Catalytic Component 1 (AGO1 Human)

   Depositor

   David Bartel

   Article

   Briskin et al Proc Natl Acad Sci U S A. 2020 Jul 28;11

   Insert

   argonaute RISC Catalytic Component 1 (AGO1 Human)
   

   Use

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 29, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-hSyn-Gluc-NHP.RMA-IRES-EGFP

   Plasmid

   #227886

   Purpose

   Expresses Gluc-NHP.RMA and EGFP under the hSyn promoter. Gluc-NHP.RMA for monitoring neuronal transduction.

   Depositor

   Jerzy Szablowski

   Article

   Lee et al bioRxiv 2025.06.01.657212; doi: 10.1101/

   Inserts

   Gaussia luciferase fused to macaque Fc
   IRES-EGFP
   

   Use

   AAV and Luciferase

   Tags

   Gluc and IgG1 Fc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-EFS-hfCas13d-HA-mCherry-pA

    Plasmid

    #233029

    Purpose

    To Express HA Tagged hfCas13d-mCherry fusion protein from the mammalian EFS promoter

    Depositor

    Jonathan Ploski

    Article

    Back et al Mol Ther Methods Clin Dev, 20 Aug 2025,

    Insert

    hfCas13d-mCherry
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-EFS-DjCas13d-P2A-mCherry-pA

    Plasmid

    #233027

    Purpose

    To Express HA tagged DjCas13d and mcherry from the mammalian EFS promoter. The DjCas13dx and mCherry are separated by a P2A site

    Depositor

    Jonathan Ploski

    Article

    Back et al Mol Ther Methods Clin Dev, 20 Aug 2025,

    Insert

    DjCas13d/mCherry
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available since

    March 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV-EFS-CasRx-P2A-mCherry-pA

    Plasmid

    #233025

    Purpose

    To Express HA tagged CasRX and mcherry from the mammalian EFS promoter. The CasRx and mCherry are separated by a P2A site

    Depositor

    Jonathan Ploski

    Article

    Back et al Mol Ther Methods Clin Dev, 20 Aug 2025,

    Insert

    CasRx/mCherry
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available since

    March 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-EFS-hfCas13d-P2A-mCherry-pA

    Plasmid

    #233026

    Purpose

    To Express HA Tagged hfCas13d and mcherry from the mammalian EFS promoter. The hfCas13d and mCherry are separated by a P2A site

    Depositor

    Jonathan Ploski

    Article

    Back et al Mol Ther Methods Clin Dev, 20 Aug 2025,

    Insert

    hfCas13d/mCherry
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available since

    March 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV-CMV-intron-CasRx-pA/EF1a-mCherry-WPRE-pa

    Plasmid

    #233032

    Purpose

    To Express HA tagged CasRX the CMV promoter. Also encodes a mCherry gene outside of the viral genome

    Depositor

    Jonathan Ploski

    Article

    Back et al Mol Ther Methods Clin Dev, 20 Aug 2025,

    Insert

    CasRx and mCherry
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available since

    March 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pPB9

    Plasmid

    #210036

    Purpose

    Contains Cas9 gRNA cloning site, inducible Cas9, UCOE, puro-T2A-eGFP

    Depositor

    Michael Talkowski

    Article

    Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

    Inserts

    Cas9
    puro-T2A-eGFP
    

    Use

    CRISPR

    Tags

    SV40 NLS and nucleoplasmin NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α and TRE3G

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. p204-U6-Switch-ON

    Plasmid

    #217883

    Purpose

    Retroviral Switch-ON vector for sgRNA expression; U6-BbsIx2-SWITCH-ON-scaffold; neoR

    Depositor

    Ulrich Elling

    Article

    Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

    Type

    Empty backbone

    Use

    CRISPR, Cre/Lox, and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    May 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-U6-DR30(sapI)-CMV-intron-hfCas13d-pA

    Plasmid

    #195865

    Purpose

    To express hfCas13d

    Depositor

    Jonathan Ploski

    Article

    Back et al Mol Ther Methods Clin Dev, 20 Aug 2025,

    Insert

    high fidelity (hf) version of CasRx (hfCas13d)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Yes: GTGGAATACATTACCAACGTGGTGTACGTG

    Promoter

    CMV

    Available since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-CMV-intron-DIO-hfCas13d-pA

    Plasmid

    #195866

    Purpose

    To express hfCas13d

    Depositor

    Jonathan Ploski

    Article

    Back et al Mol Ther Methods Clin Dev, 20 Aug 2025,

    Insert

    high fidelity (hf) version of CasRx (hfCas13d)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Yes: GTGGAATACATTACCAACGTGGTGTACGTG

    Promoter

    CMV

    Available since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. NLS-tdPCP-GFP-VP16

    Plasmid

    #183935

    Purpose

    PCP tandem dimer binding to PP7 RNA stem loops coupled to VP16 activation domain; labeled with GFP

    Depositor

    Karsten Rippe

    Article

    Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

    Insert

    tdPCP-GFP-VP16 (UL48 Synthetic)
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV (TATA box removed)

    Available since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUC57-attB2-SA(1)-CRY2-mCherry

    Plasmid

    #160440

    Purpose

    To create Cry2-mCherry lines from MiMIC lines with inserts into coding introns in Phase 1.

    Depositor

    Nicholas Tolwinski

    Article

    Bunnag et al J Mol Biol. 2020 May 1;432(10):3159-3176

    Insert

    Cryptochrome 2 (CRY2 Mustard Weed)
    

    Use

    Tags

    mCherry

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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