Addgene

We narrowed to 13,732 results for: TIM;

Showing: 1501 - 1520 of 13732 results

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pMV306hsp

Plasmid

#26155

Depositor

Siouxsie Wiles, Brian Robertson

Article

Andreu et al PLoS One. 2010 May 24;5(5):e10777.

Insert

hsp60 promoter

Use

Mycobacteria integrating vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 1, 2010

Availability

Academic Institutions and Nonprofits only

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pCAG-EGFP(HiBiT)

Plasmid

#162589

Purpose

N-terminally HiBiT-tagged EGFP under CAG promoter

Depositor

Masaharu Somiya

Article

Somiya et al Anal Chem. 2021 Mar 24. doi: 10.1021/acs

Insert

EGFP

Use

Tags

HiBiT

Expression

Mammalian

Mutation

Promoter

CAG promoter

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pSM-mStayGold

Plasmid

#234317

Purpose

codon-optimized mStayGold with synthetic introns for the expression in C. elegans

Depositor

Kota Mizumoto

Article

Ko et al MicroPubl Biol. 2025 Jan 18;2025:10.1791

Insert

C. elegans codon optimized mStayGOld

Use

Tags

mStayGold

Expression

Worm

Mutation

Promoter

Available since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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12XCSL-d1EGFP

Plasmid

#47684

Purpose

Notch reporter

Depositor

Urban Lendahl

Article

Hansson et al Dev Neurosci. 2006;28(1-2):118-27.

Insert

TP-1 promoter

Use

Notch reporter construct

Tags

d1EGFP

Expression

Mutation

hexamerized 50-bp EBNA2 response element of the T…

Promoter

NA

Available since

Feb. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pCDH-mApple

Plasmid

#179383

Purpose

Lentiviral vector for expression of mApple

Depositor

Angus Johnston

Article

Rennick et al Nat Commun. 2022 Oct 12;13(1):6023. doi:

Insert

mApple

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1

Available since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO_HaloTag7_T2A_EGFP

Plasmid

#169325

Purpose

Expression of HaloTag7 in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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12XCSL-DsRedExpressDL

Plasmid

#47683

Purpose

Notch reporter

Depositor

Urban Lendahl

Article

Hansson et al Dev Neurosci. 2006;28(1-2):118-27.

Insert

TP-1 promoter

Use

Notch reporter construct

Tags

dsRedExpressDR

Expression

Mutation

hexamerized 50-bp EBNA2 response element of the T…

Promoter

NA

Available since

Feb. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pCS-membrane-ceruleanFP

Plasmid

#53749

Purpose

Contains membrane localized cerulean fluorescent protein in pCS expression vector.

Depositor

Sean Megason

Article

Megason et al Methods Mol Biol. 2009;546:317-32. doi:

Insert

2x membrane linker

Use

Tags

Cerulean

Expression

Mammalian

Mutation

Promoter

simian CMV IE94

Available since

June 10, 2014

Availability

Academic Institutions and Nonprofits only

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pECE

Plasmid

#26453

Depositor

William Rutter

Article

Ellis et al Cell. 1986 Jun 6;45(5):721-32.

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 4, 2011

Availability

Academic Institutions and Nonprofits only

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pJKR-H-benM

Plasmid

#73925

Purpose

Muconate biosensor. Expresses GFP proportional to muconate concentration within the cell.

Depositor

George Church

Article

Rogers et al Proc Natl Acad Sci U S A. 2016 Feb 8. pi

Insert

muconate biosensing apparatus

Use

Tags

Expression

Mutation

Promoter

Available since

Jan. 14, 2017

Availability

Academic Institutions and Nonprofits only

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EC67907

Plasmid

#211794

Purpose

Over expresses Cas9 and GR-GRF-GIF. Must receive guide cassettes

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Research Square, 12 March 2024, PREPRINT

Insert

HptII, Cas9, GR-GRF-GIF

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pET51b(+)_HaloTag9

Plasmid

#169324

Purpose

Expression of HaloTag9 in bacterial cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

HaloTag9

Use

Tags

His

Expression

Bacterial

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Promoter

Available since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pHCMV-MokolaG

Plasmid

#15811

Depositor

Miguel Sena-Esteves

Article

Sena-Esteves et al J Virol Methods. 2004 Dec 15. 122(2):131

Insert

Mokola virus glycoprotein (G) gene

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 21, 2008

Availability

Academic Institutions and Nonprofits only

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pDZ276 (pURA MET28 PCP-2x-yeGFP)

Plasmid

#35194

Depositor

Robert Singer

Article

Larson et al Science. 2011 Apr 22;332(6028):475-8.

Insert

PCP-2x-yeGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 29, 2012

Availability

Academic Institutions and Nonprofits only

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pYZ183

Plasmid

#98425

Purpose

Pombe Expression Vector - nmt41 promoter with empty BsaI-pad - no marker

Depositor

Jef Boeke

Article

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pYZ184

Plasmid

#98426

Purpose

Pombe Expression Vector - nmt81 promoter with empty BsaI-pad - no marker

Depositor

Jef Boeke

Article

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX6P1 METTL3

Plasmid

#200731

Purpose

Bacterial expression of GST-Tagged codon optimized human METTL3

Depositor

Claudio Alarcón

Insert

METTL3 (METTL3 Human)

Use

Tags

GST from backbone plasmid

Expression

Bacterial

Mutation

Codon optimized for expression in E. coli

Promoter

Available since

July 25, 2023

Availability

Academic Institutions and Nonprofits only

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pPL5617_pUG_PuroR

Plasmid

#60928

Purpose

PCR template vector for generating puromycin resistance cassette.

Depositor

Robert Piper

Article

MacDonald et al Yeast. 2015 Feb 16. doi: 10.1002/yea.306

Insert

puromycin N-Acetyltransferase

Use

Loxp flanked deletion cassette template

Tags

Expression

Mutation

Promoter

TEF1

Available since

Feb. 17, 2015

Availability

Academic Institutions and Nonprofits only

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EC67908

Plasmid

#211795

Purpose

Over expresses Cas12a and GR-GRF-GIF. Must receive guide cassettes

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Research Square, 12 March 2024, PREPRINT

Insert

HptII, Cas12a, GR-GRF-GIF

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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TEV-VPR

Plasmid

#110200

Purpose

Encodes for HIV-1 VPR fused with TEV protease (TEVp)

Depositor

Sergi Padilla Parra

Article

Jones et al Sci Rep. 2015 Aug 24;5:13449. doi: 10.10

Insert

VPR, TEVp

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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