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CCM2_I432D_eGFP

Plasmid

#208879

Purpose

Express eGFP-CCM2 with the I432D mutation in mammalian cells.

Depositor

Guillaume Jacquemet

Article

Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

Insert

CCM2 (CCM2 Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

mutation I432D

Promoter

Available since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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CCM2_W421AD422A_eGFP

Plasmid

#208880

Purpose

Express eGFP-CCM2 with the W421A and D422A mutations in mammalian cells.

Depositor

Guillaume Jacquemet

Article

Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

Insert

CCM2 (CCM2 Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

mutations W421A and D422A

Promoter

Available since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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CCM2_I428S_eGFP

Plasmid

#208878

Purpose

Express eGFP-CCM2 with the I428S mutation in mammalian cells.

Depositor

Guillaume Jacquemet

Article

Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

Insert

CCM2 (CCM2 Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

mutation I428S

Promoter

Available since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmCCR4-D412AN414A_R

Plasmid

#147428

Purpose

Insect Expression of DmCCR4-D412AN414A

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmCCR4-D412AN414A (twin Fly)

Use

Tags

Expression

Insect

Mutation

The following variations comprises from our S2 ce…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pDY153: Flag(SUZ12) WT HDR plasmid

Plasmid

#170793

Purpose

WT homology directed repair plasmid for performing genome-editing on the SUZ12 Locus

Depositor

Thomas Cech

Article

Youmans et al Mol Cell. 2021 Feb 4;81(3):488-501.e9. d

Insert

SUZ12 (SUZ12 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA-Dest47- R5A-K5A N23 C11orf83-GFP

Plasmid

#65846

Purpose

Mammalian expression of the mutated N terminal part (R5A-K5A N23, transmembrane part of 23 aa) of C11orf83/UQCC3 fused to GFP protein (C-ter)

Depositor

Amos Bairoch

Article

Desmurs et al Mol Cell Biol. 2015 Apr;35(7):1139-56. d

Insert

Transmembrane (23 AA) of UQCC3 (UQCC3 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

R5A-K6A

Promoter

Available since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA-Dest47- R5D-K6D N23 C11orf83-GFP

Plasmid

#65847

Purpose

Mammalian expression of the mutated N terminal part (R5D-K5D N23, transmembrane part of 23 aa) of C11orf83/UQCC3 fused to GFP protein (C-ter)

Depositor

Amos Bairoch

Article

Desmurs et al Mol Cell Biol. 2015 Apr;35(7):1139-56. d

Insert

Transmembrane (23 AA) of UQCC3 (UQCC3 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

R5D-K6D

Promoter

Available since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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Magoh198delG

Plasmid

#30481

Depositor

Debra Silver, William Pavan

Article

Silver et al Nat Neurosci. 2010 May . 13(5):551-8.

Insert

Magoh (Magoh Mouse)

Use

Tags

Expression

Mammalian

Mutation

198delG (point nucleotide deletion)

Promoter

Available since

June 6, 2011

Availability

Academic Institutions and Nonprofits only
pMH-SFB-BRCA2

Plasmid

#99395

Purpose

Mammalian expression construct for S protein-Flag-Streptavidin binding peptide (SFB)-tagged BRCA2

Depositor

Junjie Chen, Michael Huen

Article

Sy et al Proc Natl Acad Sci U S A. 2009 Apr 28;10

Insert

BRCA2 (BRCA2 Human)

Use

Tags

SFB (S protein-FLAG-Streptavidin binding peptide)

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 29, 2017

Availability

Academic Institutions and Nonprofits only

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EGFP-CAAX

Plasmid

#86056

Purpose

A mammalian expression plasmid encoding farnesylation motif of HRAS with N-terminus EGFP.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

Homo sapiens Harvey rat sarcoma (HRAS Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

contains the region from 825-857 of HRAS as a far…

Promoter

CMV

Available since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

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pGART-GFP

Plasmid

#99106

Purpose

Expresses human GART-GFP fusion in mammalian cells

Depositor

Stephen Benkovic

Article

An et al Science. 2008 Apr 4;320(5872):103-6. doi

Insert

GART (GART Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

A65T, L68V, and A72S mutations in GFP gene; kanR/…

Promoter

CMV

Available since

Dec. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/Ndi-1/myc-His

Plasmid

#127503

Purpose

Expresses myc-tagged yeast Ndi-1 in mammalian cells

Depositor

Shashi Jain, Dieter Wolf

Article

Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

Insert

Ndi-1 (NDI1 Budding Yeast)

Use

Tags

myc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/Flag-KIAA1267

Plasmid

#29758

Depositor

Joan Conaway

Article

Cai et al J Biol Chem. 2010 Feb 12. 285(7):4268-72

Insert

KIAA1267 (PHF20, KANSL1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 30, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.1/NDUFS7/myc-His

Plasmid

#127488

Purpose

Expresses myc-tagged NDUFS7 in mammalian cells

Depositor

Shashi Jain, Dieter Wolf

Article

Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

Insert

NDUFS7 (NDUFS7 Human)

Use

Tags

myc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 16, 2019

Availability

Academic Institutions and Nonprofits only

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CD-MPR-GFP-RUSH

Plasmid

#202797

Purpose

RUSH cargo: Str-KDEL_IRES_SBP-EGFP-CD-MPR

Depositor

Juan Bonifacino

Article

Chen et al J Cell Biol. 2017 Dec 4;216(12):4141-415

Insert

M6PR (M6PR Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Changed 40 K (AAA) with R (AGA)

Promoter

CMV

Available since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/NDUFS2/myc-His

Plasmid

#127486

Purpose

Expresses myc-tagged NDUFS2 in mammalian cells

Depositor

Shashi Jain, Dieter Wolf

Article

Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

Insert

NDUFS2 (NDUFS2 Human)

Use

Tags

myc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 17, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-mGas8-PA

Plasmid

#176468

Purpose

Expression vector of mouse growth arrest specific 8 (Gas8) tagged with PA at C-terminus.

Depositor

Masahito Ikawa

Article

Morohoshi et al PLoS Genet. 2020 Jan 21;16(1):e1008585.

Insert

growth arrest specific 8 (Gas8 Mouse)

Use

Tags

PA

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

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CD-MPR-mCherry-RUSH

Plasmid

#202798

Purpose

RUSH cargo: Str-KDEL_IRES_SBP-mCherry-CD-MPR

Depositor

Juan Bonifacino

Article

Chen et al J Cell Biol. 2017 Dec 4;216(12):4141-415

Insert

M6PR (M6PR Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Changed 40 K (AAA) with R (AGA)

Promoter

CMV

Available since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/NDUFV2/myc-His

Plasmid

#127491

Purpose

Expresses myc-tagged NDUFV2 in mammalian cells

Depositor

Shashi Jain, Dieter Wolf

Article

Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

Insert

NDUFV2 (NDUFV2 Human)

Use

Tags

myc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 16, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/Flag-FLJ10081

Plasmid

#64775

Purpose

Mammalian expression of Flag-tagged FLJ10081 (NSL3)

Depositor

Joan Conaway, Ronald Conaway

Article

Cai et al J Biol Chem. 2010 Feb 12. 285(7):4268-72

Insert

FLJ10081 (KANSL3 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

May 19, 2015

Availability

Academic Institutions and Nonprofits only

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