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KHLH31 pEGFP-N2 (1089)

Plasmid

#62032

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

Insert

KHLH31

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only
pFAB806

Plasmid

#47849

Purpose

BIOFAB reporter plasmid for measuring lambda tR2 termination efficiency

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

lambda tR2 terminator

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLTetO4

Available Since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pFAB805

Plasmid

#47850

Purpose

BIOFAB reporter plasmid for measuring tonB termination efficiency

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

tonB terminator

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLTetO5

Available Since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pFAB816

Plasmid

#47851

Purpose

BIOFAB reporter plasmid for measuring ilvGEDA termination efficiency

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

ilvGEDA

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLTetO6

Available Since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pFAB807

Plasmid

#47852

Purpose

BIOFAB reporter plasmid for measuring RNAI termination efficiency

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

RNAI terminator

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLTetO7

Available Since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pFAB820

Plasmid

#47855

Purpose

BIOFAB reporter plasmid for measuring tetAC termination efficiency

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

tetAC terminator

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLTetO10

Available Since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pFAB1910

Plasmid

#47859

Purpose

BIOFAB reporter plasmid for measuring rpoC termination efficiency

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

rpoC terminator

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLTetO14

Available Since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pFAB804

Plasmid

#47848

Purpose

BIOFAB reporter plasmid for measuring T3 early termination efficiency

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

T3 early terminator

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLTetO3

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pFAB3905

Plasmid

#47808

Purpose

BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD1 efficiency.

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

promoter 14 and BCD1

Use

Synthetic Biology

Expression

Bacterial

Promoter

see insert

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pZT-AAVS1-R1

Plasmid

#52638

Purpose

TALEN-mediated gene editing at human PPP1R12C/AAVS1 locus

Depositor

Mahendra Rao, Jizhong Zou

Article

Luo et al Stem Cells Transl Med. 2014 May 15. pii:

Insert

Right AAVS1 TALEN

Use

TALEN

Expression

Mammalian

Promoter

CMV

Available Since

May 12, 2014

Availability

Academic Institutions and Nonprofits only

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pZT-AAVS1-L1

Plasmid

#52637

Purpose

TALEN-mediated gene editing at human PPP1R12C/AAVS1 locus

Depositor

Mahendra Rao, Jizhong Zou

Article

Luo et al Stem Cells Transl Med. 2014 May 15. pii:

Insert

Left AAVS1 TALEN

Use

TALEN

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pOTTC407 - pAAV EF1a eGFP

Plasmid

#60058

Purpose

An AAV packaging vector that expresses enhanced green fluorescent protein under control of the EF1a promoter.

Depositor

Brandon Harvey

Article

Savolainen et al Neurobiol Dis. 2014 Aug;68:1-15. doi: 10

Insert

enhanced Green Fluorescent Protein

Use

AAV

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 9, 2014

Availability

Academic Institutions and Nonprofits only

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TNPO1-shRNA

Plasmid

#86082

Purpose

A lentiviral construct for building stable cell lines expressing Transportin-1 shRNA via puromycin selection.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

Transportin-1 (TNPO1 Human)

Expression

Mammalian

Promoter

U6

Available Since

May 26, 2017

Availability

Academic Institutions and Nonprofits only

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PCMV-intron myc Rab1a S25N

Plasmid

#46777

Purpose

expression of S25N Rab1a in mammalian cells

Depositor

Terry Hébert

Article

Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

Insert

Rab1a S25N mutant (RAB1A Human)

Tags

myc

Expression

Mammalian

Mutation

S25N (dominant negative)

Promoter

CMV

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only
NET5s pEGFP-N2 (1154)

Plasmid

#62034

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Malik et al Cell Mol Life Sci. 2010 Apr;67(8):1353-6

Insert

NET5s (TMEM201 Human)

Tags

EGFP

Expression

Mammalian

Mutation

short form

Promoter

CMV

Available Since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only
NET5L pEGFP-N2 (1155)

Plasmid

#62035

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

Insert

NET5L (TMEM201 Human)

Tags

EGFP

Expression

Mammalian

Mutation

long form

Promoter

CMV

Available Since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only
pAAV-hsyn-flex-dsRed-shvgat

Plasmid

#67845

Purpose

Expresses shRNA to the vesicular GABA transporter VGAT. Cre dependent expression by flex switch

Depositor

William Wisden

Article

Yu et al Neuron. 2015 Jun 17. pii: S0896-6273(15)

Insert

AAV-hsyn-flexed-dsRed-miR30-vgat2.shRNA

Use

AAV, Cre/Lox, Mouse Targeting, and RNAi

Expression

Mammalian

Promoter

h-synapsin

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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EGFP-hPMCA2w/b

Plasmid

#47586

Depositor

Emanuel Strehler

Article

Chicka et al J Biol Chem. 2003 May 16;278(20):18464-7

Insert

PMCA2w/b (ATP2B2 Human)

Tags

EGFP-HA-His

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pAct-FRT-stop-FRT3-FRT-FRT3-Gal4 attB

Plasmid

#52889

Purpose

"CoinFLP-Gal4" - Expresses Gal4 from actin promoter downsteam of transcriptional stop. Recombination by FLP excises FRT-FRT pair to express Gal4, or FRT3-FRT3 pair to prevent Gal4 expression

Depositor

Iswar Hariharan

Article

Bosch et al Development. 2015 Feb 1;142(3):597-606.

Insert

Gal4 (GAL4 Budding Yeast)

Expression

Insect

Available Since

Jan. 22, 2015

Availability

Academic Institutions and Nonprofits only

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Tmem38A pEGFP-N2 (1401)

Plasmid

#62050

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

Insert

Tmem38A (TMEM38A Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only