We narrowed to 1,045 results for: mEGFP

1. pET28-RPL3-212~285-mEGFP(A206K)-TwStrep

   Plasmid

   #208629

   Purpose

   for E. coli expression of truncated human RPL3 (ENST00000216146), aa212~285, fused with mEGFP(A206K) and Twin Strep tag

   Depositor

   Quanfu Ma

   Insert

   human RPL3 (ENST00000216146) aa 212~285 (RPL3 Human)
   

   Use

   Tags

   6xHis, Twin Strep, and mEGFP(A206K)

   Expression

   Bacterial

   Mutation

   only aa 212~285, other residues not included

   Promoter

   T7

   Available since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET29b-AviTag-mEGFP-dspB(E184Q W330Y)-6xHis

   Plasmid

   #176575

   Purpose

   Plasmid for overexpression of recombinant AviTagged, His-tagged fusion protein mEGFP-DspB(E184Q W330Y), used as a non-binding control for detection of biofilm polysaccharide PNAG.

   Depositor

   Mark Nitz

   Article

   Eddenden et al Methods Enzymol. 2022;665:209-231. doi:

   Insert

   Dispersin B
   

   Use

   Tags

   Avitag, Hexahistidine tag, and mEGFP

   Expression

   Bacterial

   Mutation

   E184Q W330Y

   Promoter

   T7

   Available since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1D/mEGFP + CrPV IGR IRES-nLuc-3XFLAG-CL1PEST

   Plasmid

   #127332

   Purpose

   Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from CrPV IRES

   Depositor

   Jeremy Wilusz

   Article

   Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

   Insert

   nanoLuciferase
   

   Use

   Tags

   3xFLAG and CL1/PEST

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCru5-ACC/ACC/ACC/ACC-mEGFP-IRES-mCherry

   Plasmid

   #49218

   Purpose

   Retroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.

   Depositor

   Clifford Wang

   Article

   Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110

   Inserts

   mEGFP
   mCherry
   

   Use

   Retroviral and Synthetic Biology

   Tags

   SfuI site to create C-terminal fusions

   Expression

   Mammalian

   Mutation

   Promoter

   Viral LTR (or CMV)

   Available since

   Dec. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pWPT-ACC/ACC/ACC/ACC-mEGFP-IRES-mCherry

   Plasmid

   #49227

   Purpose

   Lentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.

   Depositor

   Clifford Wang

   Article

   Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110

   Inserts

   mEGFP
   mCherry
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   SfuI site to create C-terminal fusions

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-short

   Available since

   Dec. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA5/FRT-Lyn11-mEGFP-cpHalo∆-(GGS)9-FKBP-P2A-Hpep3-(GGS)3-FRB-mScarlett

   Plasmid

   #205703

   Purpose

   CMV driven co-expression of split HaloTag FKBP- and FRB-fusions (Lyn11-EGFP-FKBP-cpHalo∆ and Hpep1-FRB-mScarlett) in mammalian cell lines

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Lyn11-EGFP-FKBP-cpHalo∆-P2A-Hpep1-FRB-mScarlett
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti_EF1α-NES-Kinprola_PKA-mEGFP_bGH-PA term_EF1α_FLAG-SV40NLS-Cas9-NLS-T2A-BSD-WPRE-SV40

   Plasmid

   #233371

   Purpose

   EF1α driven co-expression of the PKA activity recorder Kinprola_PKA fused to mEGFP and Cas9 expression through lentivirus transduction

   Depositor

   Kai Johnsson

   Article

   Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

   Inserts

   NES-Kinprola_PKA-mEGFP
   FLAG-SV40NLS-Cas9-NLS-T2A-BSD
   

   Use

   Lentiviral

   Tags

   FLAG, NES, and mEGFP

   Expression

   Mutation

   Promoter

   EF1α

   Available since

   March 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti_EF1α-NES-Kinprola_on-mEGFP_bGH-PA term_EF1α_FLAG-SV40NLS-Cas9-NLS-T2A-BSD-WPRE-SV40

   Plasmid

   #233373

   Purpose

   EF1α driven co-expression of the consitutively active kinase activity recorder positive control Kinprola_on fused to mEGFP and Cas9 expression through lentivirus transduction

   Depositor

   Kai Johnsson

   Article

   Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

   Inserts

   NES-Kinprola_on-mEGFP
   FLAG-SV40NLS-Cas9-NLS-T2A-BSD
   

   Use

   Lentiviral

   Tags

   FLAG, NES, and mEGFP

   Expression

   Mutation

   Promoter

   EF1α

   Available since

   March 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti_EF1α-NES-Kinprola_PKA_T/A-mEGFP_bGH-PA term_EF1α_FLAG-SV40NLS-Cas9-NLS-T2A-BSD-WPRE-SV40

   Plasmid

   #233372

   Purpose

   EF1α driven co-expression of the inactive PKA activity recorder negative control with T/A mutation Kinprola_PKA_T/A fused to mEGFP and Cas9 expression through lentivirus transduction

   Depositor

   Kai Johnsson

   Article

   Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

   Inserts

   NES-Kinprola_PKA_T/A-mEGFP
   FLAG-SV40NLS-Cas9-NLS-T2A-BSD
   

   Use

   Lentiviral

   Tags

   FLAG, NES, and mEGFP

   Expression

   Mutation

   Promoter

   EF1α

   Available since

   March 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFA6a-HaloTag-KanMX6

    Plasmid

    #87029

    Purpose

    Tag S. pombe genes in C terminal with HaloTag

    Depositor

    Julien Berro

    Insert

    HaloTag
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Feb. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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