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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 5,856 results for: 129

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Showing: 1521 - 1540 of 5856 results

  2. pCryptDel4.8

    Plasmid
    #141293
    Purpose
    Plasmid for curing pMUT2 in one step based on pFREE. Contains RelB antitoxin, as well as gRNA targetting pMUT2 plasmid as well as pCryptDel4.8 itself.
    Depositor
    Neel Joshi
    Article
    Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102
    Inserts
    RelB
    gRNA targetting pMUT2
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    Native promoter from pMUT2 relB/relE operon and P…
    Available Since
    Jan. 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    141293-plasmid-map-sequence-id-322822

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  3. pANAP-P2A-eRF(E55D)

    Plasmid
    #241290
    Purpose
    Allows co-translational incorporation of the fluorescent ncAA Anap directly into the target protein; expresses tRNACUA-Leu, AnapRS, and dominant negative ribosomal release factor
    Depositor
    Justin Taraska
    Article
    Obashi et al Structure. Volume 34, Issue 1, 8 January
    Insert
    the amber suppressor tRNACUA-Leu
    Expression
    Mammalian
    Mutation
    mutant E.coli leucyl synthetase specific for Anap…
    Promoter
    CMV
    Available Since
    Dec. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    241290-plasmid-map-sequence-id-481082

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  11. rd

    Plasmid
    #112912
    Purpose
    Express the PEST domain-replaced 48 kDa form of human dematin with a cleavable N-terminal GST tag
    Depositor
    C. James McKnight
    Article
    Chen et al J Biol Chem. 2013 Mar 22;288(12):8313-20
    Insert
    dematin (DMTN Human)
    Tags
    Glutatione-S-Tranferase and precision protease si…
    Expression
    Bacterial
    Mutation
    PEST sequence near the N-terminal removed. 5′-GCG…
    Promoter
    T7
    Available Since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    112912-plasmid-map-sequence-id-220213

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  12. rd-his

    Plasmid
    #112911
    Purpose
    Express the PEST domain-replaced 48 kDa form of human dematin with a cleavable N-terminal GST tag and non-cleavable C-terminal 6-his tag
    Depositor
    C. James McKnight
    Article
    Chen et al J Biol Chem. 2013 Mar 22;288(12):8313-20
    Insert
    dematin (DMTN Human)
    Tags
    6-his tag, Glutathione-S-Transferase, and preciss…
    Expression
    Bacterial
    Mutation
    89KSTSPPPSPEVWAD102 was replaced with 89KAAAGGGAG…
    Promoter
    T7
    Available Since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    112911-plasmid-map-sequence-id-220214

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  14. PD-L1-NanoLuc Fusion Vector

    Plasmid
    #237129
    Purpose
    Express PD-L1-NanoLuc(R) Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    PD-L1 (CD274 Human)
    Tags
    NanoLuc (R)
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    237129-plasmid-map-sequence-id-474957

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Showing: 1521 - 1540 of 5856 results