We narrowed to 5,304 results for: Ado;

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EF1a_SOX3_P2A_Hygro_Barcode

Plasmid

#120481

Purpose

Barcoded lentiviral vector to express SOX3 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

SOX3 (SOX3 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pAC84-pCR8-dCas9

Plasmid

#48218

Purpose

dCas9 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Insert

dCas9(D10A;H840A)

Use

CRISPR and Gateway Cloning

Tags

HA Tag

Mutation

D10A;H840A

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pFETCh_CEBPA

Plasmid

#86293

Purpose

Donor vector for 3' FLAG tag of human CEBPA

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

CEBPA homology arms (CEBPA Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_AHR_2

Plasmid

#101077

Purpose

Encodes gRNA for 3' target of human AHR

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against AHR (AHR Human)

Use

CRISPR

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/TO-UBE2QL1-Strep/HA

Plasmid

#124666

Purpose

Expresses UBE2QL1 with C-terminal Strep-HA tag in mammalian cells

Depositor

Hemmo Meyer

Article

Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

Insert

UBE2QL1 (UBE2QL1 Human)

Tags

2xStrep/HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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2xHP1bCD-IFP2.0C

Plasmid

#215750

Purpose

Expresses double domain of HP1beta chromo domain fused to C-terminal part of IFP2.0 for H3K9me2/3 detection in BiAD sensors

Depositor

Albert Jeltsch

Article

Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

Insert

2xHP1bCD-IFP2.0C

Expression

Mammalian

Promoter

CMV

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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2xHP1bCD_W42A-IFP2.0C

Plasmid

#215751

Purpose

Expresses binding-deficient W42A mutant of 2xHP1beta chromo domain fused to C-terminal part of IFP2.0 as negative control for H3K9me2/3 detection in BiAD sensors

Depositor

Albert Jeltsch

Article

Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

Insert

2xHP1bCD_W42A-IFP2.0C

Expression

Mammalian

Promoter

CMV

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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EF1a_MYOG_P2A_Hygro_Barcode

Plasmid

#120465

Purpose

Barcoded lentiviral vector to express MYOG in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

MYOG (MYOG Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/TO-UBE2QL1-CS-Strep/HA

Plasmid

#129513

Purpose

Expresses UBE2QL1 C88S (catalytically inactive mutant) with C-terminal Strep-HA tag in mammalian cells

Depositor

Hemmo Meyer

Article

Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

Insert

UBE2QL1 C88S (UBE2QL1 Human)

Tags

2xStrep/HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pAC5-dual-dCas9VP48-sgTetO

Plasmid

#48237

Purpose

Dual expression construct expressing both dCas9VP48 and sgTetO from separate promoters

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Insert

dCas9VP48 and sgTetO

Use

CRISPR

Tags

HA-tag and VP48

Expression

Mammalian

Mutation

D10A H840A (catalytically inactive)

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pCDF11-SFPQ(276-598)

Plasmid

#135437

Purpose

Expresses SFPQ 276-598 with TEV-cleavable hexahistidine tag

Depositor

Charles Bond

Article

Lee et al Nucleic Acids Res. 2015 Apr 20;43(7):382

Insert

Splicing Factor Proline And Glutamine Rich (SFPQ Human)

Tags

hexahistidine

Expression

Bacterial

Promoter

T7

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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PX458_KDM6A_1

Plasmid

#86307

Purpose

Encodes gRNA for 3' target of human KDM6A

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against KDM6A (KDM6A Human)

Use

CRISPR

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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U6-Slc1a3 sgRNA; EF1a-dCas9-KRAB-GFP

Plasmid

#194283

Purpose

EF1a-dCas9-KRAB-GFP with Slc1a3 sgRNA

Depositor

Elizabeth Kirby

Article

Rieskamp et al iScience. 2023 Jun 8;26(7):107068. doi:

Insert

dCas9-KRAB-GFP

Use

CRISPR and Lentiviral

Tags

GFP

Promoter

EF1a

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pFETCh_ARID5B

Plasmid

#86288

Purpose

Donor vector for 3' FLAG tag of human ARID5B

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ARID5B homology arms (ARID5B Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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GFP-C-CARMIL2b

Plasmid

#118741

Purpose

To express CARMIL2 with GFP tag on N-terminus

Depositor

John Cooper

Article

Lanier et al Mol Biol Cell. 2015 Dec 15;26(25):4577-8

Insert

CARMIL2 (CARMIL2 Human)

Tags

AcGFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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mCherry-Climp63(1–192)

Plasmid

#186955

Purpose

Expresses mCherry-Climp63 with partial luminal domain

Depositor

Ke Xu

Article

Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

Insert

Ckap4 (Ckap4 Mouse)

Tags

mCherry

Expression

Mammalian

Mutation

Only remain partial luminal domain

Promoter

CMV Promoter

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2+ Zf cdh1-deltaCyot-GFP

Plasmid

#185412

Purpose

GFP tagged zebrafish cdh1 lacking the cytoplasmic domain.

Depositor

Nicolas David

Article

Boutillon et al Dev Cell. 2022 May 17. pii: S1534-5807(2

Insert

cdh1 (cdh1 Zebrafish)

Use

In vitro synthesis of mrna

Tags

eGFP

Expression

Mammalian

Mutation

Cytoplasmic domain deleted

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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PX458_KDM6A_2

Plasmid

#86308

Purpose

Encodes gRNA for 3' target of human KDM6A

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against KDM6A (KDM6A Human)

Use

CRISPR

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pFETCh_MIER3

Plasmid

#86274

Purpose

Donor vector for 3' FLAG tag of human MIER3

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

MIER3 homology arms (MIER3 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pFETCh_FOXP1

Plasmid

#86261

Purpose

Donor vector for 3' FLAG tag of human FOXP1

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

FOXP1 homology arms (FOXP1 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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